- 商品货号:FH1618
- 规格:10μl质粒
- 终止子:SV40 poly(A) signal
- 质粒大小:34947bp
- 原核抗性:氨苄青霉素Amp
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: pacAd5 9.2-100是一个腺病毒骨架质粒,用于产生腺病毒,pacAd5 9.2-100质粒的参考资料是:RAPAd Universal Adenoviral Expression System(点击查看)
Recombinant adenoviruses have tremendous potential in both research and therapeutic applications.There are numerous advantages they provide when introducing genetic material into host cells. The permissive host cell range is very wide. The virus has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e.does not integrate into the host chromosome so does not activate or inactivate host genes). Recently, recombinant adenoviruses have been used to deliver RNAi into cells. Two methods have traditionally been used to generate recombinant adenoviruses. The first involves homologous recombination of a shuttle vector containing gene of interest and an adenoviral backbone plasmid vector (restricted in E1/E3) in an adenovirus packaging cell line. The isolation of recombinant adenovirus by this method involves performing multiple plaque isolations to avoid wild-type virus and is extremely laborious and time consuming. The second method, pAdEasy system, employs the homologous recombination machinery in E. coli, a recombinant adenovirus is produced by a doublerecombination event between cotransformed adenoviral backbone plasmid vector and a shuttle vector carrying the gene of interest. For the pAdEasy method, the system is high fidelity, but inefficient and requires the screening of many bacterial colonies. This results in a significant time commitment even before transfection of recombinant DNA into E1-expressing cells such as HEK293 cells.- 质粒图谱1: png/1-1G1050924134R.png
质粒序列: 点击查看序列LOCUS Exported 34947 bp ds-DNA circular SYN 05-NOV-2017DEFINITION synthetic circular DNAORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 34947)http://www.fenghbio.cnFEATURES Location/Qualifierssource 1..34947/organism="synthetic DNA construct"/mol_type="other DNA"polyA_signal 34..168/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"misc_feature 169..32952/label=ADmisc_feature 4172..4275/label=Ad SA/note="adenovirus major late transcript splice acceptor(Friedrich and Soriano, 1991)"repeat_region 32850..32952/label=ITR/note="inverted terminal repeat of human adenovirusserotype 5"rep_origin complement(33143..33731)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"misc_feature complement(33882..34742)/label=Amppromoter complement(34743..34847)/gene="bla"/label=AmpR promoterORIGIN1 ttaattaagc tagagcggcc gccaccgcgg ggagatccag acatgataag atacattgat61 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt121 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat181 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa241 aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg301 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg361 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg421 cagccatgag gtcgactcta gtccccgcgg tggcagatct ggaaggtgct gaggtacgat481 gagacccgca ccaggtgcag accctgcgag tgtggcggta aacatattag gaaccagcct541 gtgatgctgg atgtgaccga ggagctgagg cccgatcact tggtgctggc ctgcacccgc601 gctgagtttg gctctagcga tgaagataca gattgaggta ctgaaatgtg tgggcgtggc661 ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat ctgttttgca721 gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag ctcatatttg781 acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc cagcattgat841 ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt gtctggaacg901 ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt961 gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc1021 cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat1081 gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc1141 cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag1201 caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg1261 tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg1321 ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca1381 tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta1441 aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca1501 aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt1561 tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc1621 agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg1681 aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg1741 gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag1801 ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca1861 gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt1921 tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg1981 gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta2041 ccgcagccgg tgggcccgta aatcacacct attaccggct gcaactggta gttaagagag2101 ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc2161 atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc2221 aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt2281 tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc2341 agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct2401 cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct2461 gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg2521 tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga2581 ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg2641 aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa2701 ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca2761 cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga2821 tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt2881 ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct2941 cgtatagaaa ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg3001 ctaagtggga ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa3061 gacacatgtc gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt3121 gaccgggtgt tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct3181 cttccgcatc gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg3241 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct3301 ggcccgcggt gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt3361 tgttgtcaag cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg3421 agcgcagggt ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca3481 cgtattcgcg cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca3541 ggtgcacgcg ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc3601 cgcgtaggcg ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta3661 gggggtctag ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca3721 ggcgcgcgtc gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc3781 gggcggcaag cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga3841 gcgcggaggc gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa3901 gatatgtagg gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt3961 gcgagggagc gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga4021 ctatctgcct gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga4081 agctggcgtc tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct4141 tgttgaccag ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga4201 tgatgtcata cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt4261 cgcggtcttt ccagtactct tggatcggaa acccgtcggc ctccgaacgg taagagccta4321 gcatgtagaa ctggttgacg gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt4381 atgcctgcgc ggccttccgg agcgaggtgt gggtgagcgc aaaggtgtcc ctgaccatga4441 ctttgaggta ctggtatttg aagtcagtgt cgtcgcatcc gccctgctcc cagagcaaaa4501 agtccgtgcg ctttttggaa cgcggatttg gcagggcgaa ggtgacatcg ttgaagagta4561 tctttcccgc gcgaggcata aagttgcgtg tgatgcggaa gggtcccggc acctcggaac4621 ggttgttaat tacctgggcg gcgagcacga tctcgtcaaa gccgttgatg ttgtggccca4681 caatgtaaag ttccaagaag cgcgggatgc ccttgatgga aggcaatttt ttaagttcct4741 cgtaggtgag ctcttcaggg gagctgagcc cgtgctctga aagggcccag tctgcaagat4801 gagggttgga agcgacgaat gagctccaca ggtcacgggc cattagcatt tgcaggtggt4861 cgcgaaaggt cctaaactgg cgacctatgg ccattttttc tggggtgatg cagtagaagg4921 taagcgggtc ttgttcccag cggtcccatc caaggttcgc ggctaggtct cgcgcggcag4981 tcactagagg ctcatctccg ccgaacttca tgaccagcat gaagggcacg agctgcttcc5041 caaaggcccc catccaagta taggtctcta catcgtaggt gacaaagaga cgctcggtgc5101 gaggatgcga gccgatcggg aagaactgga tctcccgcca ccaattggag gagtggctat5161 tgatgtggtg aaagtagaag tccctgcgac gggccgaaca ctcgtgctgg cttttgtaaa5221 aacgtgcgca gtactggcag cggtgcacgg gctgtacatc ctgcacgagg ttgacctgac5281 gaccgcgcac aaggaagcag agtgggaatt tgagcccctc gcctggcggg tttggctggt5341 ggtcttctac ttcggctgct tgtccttgac cgtctggctg ctcgagggga gttacggtgg5401 atcggaccac cacgccgcgc gagcccaaag tccagatgtc cgcgcgcggc ggtcggagct5461 tgatgacaac atcgcgcaga tgggagctgt ccatggtctg gagctcccgc ggcgtcaggt5521 caggcgggag ctcctgcagg tttacctcgc atagacgggt cagggcgcgg gctagatcca5581 ggtgatacct aatttccagg ggctggttgg tggcggcgtc gatggcttgc aagaggccgc5641 atccccgcgg cgcgactacg gtaccgcgcg gcgggcggtg ggccgcgggg gtgtccttgg5701 atgatgcatc taaaagcggt gacgcgggcg agcccccgga ggtagggggg gctccggacc5761 cgccgggaga gggggcaggg gcacgtcggc gccgcgcgcg ggcaggagct ggtgctgcgc5821 gcgtaggttg ctggcgaacg cgacgacgcg gcggttgatc tcctgaatct ggcgcctctg5881 cgtgaagacg acgggcccgg tgagcttgaa cctgaaagag agttcgacag aatcaatttc5941 ggtgtcgttg acggcggcct ggcgcaaaat ctcctgcacg tctcctgagt tgtcttgata6001 ggcgatctcg gccatgaact gctcgatctc ttcctcctgg agatctccgc gtccggctcg6061 ctccacggtg gcggcgaggt cgttggaaat gcgggccatg agctgcgaga aggcgttgag6121 gcctccctcg ttccagacgc ggctgtagac cacgccccct tcggcatcgc gggcgcgcat6181 gaccacctgc gcgagattga gctccacgtg ccgggcgaag acggcgtagt ttcgcaggcg6241 ctgaaagagg tagttgaggg tggtggcggt gtgttctgcc acgaagaagt acataaccca6301 gcgtcgcaac gtggattcgt tgatatcccc caaggcctca aggcgctcca tggcctcgta6361 gaagtccacg gcgaagttga aaaactggga gttgcgcgcc gacacggtta actcctcctc6421 cagaagacgg atgagctcgg cgacagtgtc gcgcacctcg cgctcaaagg ctacaggggc6481 ctcttcttct tcttcaatct cctcttccat aagggcctcc ccttcttctt cttctggcgg6541 cggtggggga ggggggacac ggcggcgacg acggcgcacc gggaggcggt cgacaaagcg6601 ctcgatcatc tccccgcggc gacggcgcat ggtctcggtg acggcgcggc cgttctcgcg6661 ggggcgcagt tggaagacgc cgcccgtcat gtcccggtta tgggttggcg gggggctgcc6721 atgcggcagg gatacggcgc taacgatgca tctcaacaat tgttgtgtag gtactccgcc6781 gccgagggac ctgagcgagt ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc6841 taaccagtca cagtcgcaag gtaggctgag caccgtggcg ggcggcagcg ggcggcggtc6901 ggggttgttt ctggcggagg tgctgctgat gatgtaatta aagtaggcgg tcttgagacg6961 gcggatggtc gacagaagca ccatgtcctt gggtccggcc tgctgaatgc gcaggcggtc7021 ggccatgccc caggcttcgt tttgacatcg gcgcaggtct ttgtagtagt cttgcatgag7081 cctttctacc ggcacttctt cttctccttc ctcttgtcct gcatctcttg catctatcgc7141 tgcggcggcg gcggagtttg gccgtaggtg gcgccctctt cctcccatgc gtgtgacccc7201 gaagcccctc atcggctgaa gcagggctag gtcggcgaca acgcgctcgg ctaatatggc7261 ctgctgcacc tgcgtgaggg tagactggaa gtcatccatg tccacaaagc ggtggtatgc7321 gcccgtgttg atggtgtaag tgcagttggc cataacggac cagttaacgg tctggtgacc7381 cggctgcgag agctcggtgt acctgagacg cgagtaagcc ctcgagtcaa atacgtagtc7441 gttgcaagtc cgcaccaggt actggtatcc caccaaaaag tgcggcggcg gctggcggta7501 gaggggccag cgtagggtgg ccggggctcc gggggcgaga tcttccaaca taaggcgatg7561 atatccgtag atgtacctgg acatccaggt gatgccggcg gcggtggtgg aggcgcgcgg
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