- 商品货号:FH1806
- 规格:10μl质粒
- 启动子:35S
- 原核抗性:卡那霉素Kan
- 筛选标记:遗传霉素G418
- 克隆菌株:大肠杆菌HB101
- 质粒简介: pRI201-ON是一个单子叶植物发根农杆菌的双表达质粒。The Ri Plasmid pRI201 is designed for the transformation and expression of target genes from plant cells. This series of binary vectors for plant transformation retains the backbone of the pRI101 vectors, including an alcohol dehydrogenase (ADH) gene-derived 5' untranslated region (5'-UTR) downstream of the cauliflower mosaic virus (CaMV promoter)-derived 35S promoter. Additionally, these vectors have a heat shock protein (HSP) gene-derived terminator in place of the nopaline synthase (NOS) gene-derived terminator, allowing higher target gene expression compared with the pRI101 vector series. Multigene transformation with a single vector can be achieved by integrating an expression cassette containing another gene (promoter + enhancer + gene of interest + terminator) into the vector's second multiple cloning site (MCS2) located downstream of the HSP terminator.
The Ri Plasmid pRI201 binary vector series offers two types of vectors: pRI 201-AN DNA and pRI 201-ON DNA. pRI 201-AN DNA contains an Arabidopsis ADH-derived 5' UTR (AtADH 5'-UTR) and is suitable for dicotyledonous plant transformation. pRI 201-ON DNA contains a rice ADH-derived 5' UTR (OsADH 5'-UTR) and is suitable for monocotyledonous plant transformation. The pRI 201-AN DNA and pRI 201-ON DNA binary vectors for plant transformation have a mutant-type replication origin (Ri ori) from the Rhizobium rhizogenes Ri plasmid. These vectors also contain a replication origin (ColE1 ori) derived from the pUC plasmid; the ColE1 ori allows high copy number replication in E. coli. The vectors' multiple cloning site, located near the right border (RB) of T-DNA, allows stable target gene integration into the plant chromosome.- 质粒图谱1: gif/1-1P106120534945.gif
质粒序列: 点击查看序列LOCUS Exported 10475 bp ds-DNA circular SYN 23-NOV-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..10475/organism="synthetic DNA construct"/mol_type="other DNA"rep_origin complement(57..645)/direction=LEFT/label=ori/note="high-copy-number colE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(6419..7213)/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=NeoR/KanR/note="confers resistance to neomycin, kanamycin, and G418(Geneticin)"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"promoter 7620..7650/label=lac promoter/note="promoter for the E. coli lac operon"protein_bind 7658..7674/label=lac operator/bound_moiety="lac repressor encoded by lacI"/note="The lac repressor binds to the lac operator toinhibit transcription in E. coli. This inhibition can berelieved by adding lactose orisopropyl-beta-D-thiogalactopyranoside (IPTG)."primer_bind 7682..7698/label=M13 rev/note="common sequencing primer, one of multiple similarvariants"primer_bind complement(9075..9091)/label=M13 fwd/note="common sequencing primer, one of multiple similarvariants"ORIGIN1 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc61 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcga tcctacaagg901 tagaatccgc ctgagtcgca agggtgactt cgcctatatt ggacgacggc gcgcagaggg961 cgacctcttt ttgggttacg attgtaggat tatcactaaa acaatacatg aacatattca1021 aatggcaatc tctctaaggc attggaaata aatacaaata acagttgggt ggagtttttc1081 gacctgaggg cgttaacctt ctgttaacct aaaagctctt gcccaaacag cagaatcggc1141 gctaattgcc agcggcggaa cttttccagt ttcgcgaaaa atatcgccac tggcaaggaa1201 tgggtttgag atggcgaagt ctgtcctaaa agcagcgcct gtagttgtag ggttgacggc1261 cttgatggag cgtcatgccg atgccctctc gagccaactt caagcacatc atcttaaggt1321 tttcccgccg cattccgaga agggtattcg aacattcggg ccatcggagg cgtccaagct1381 gctcggcgtt ggcgagtcat atttacggca gaccgcgtct gagatgccag agttgaatgt1441 tagcatgagc ccaggtggca ggcgaatgtt ctcaattgaa gatatccatg tgattcggaa1501 gtatatggat caggtcggcc gcgggaaccg gcgctacctg ccacatcgtc gaggcggcga1561 gcagcttcag gttatctctg tgatgaattt caaaggtggg tcgggtaaga ccaccaccgc1621 cgcgcatctg gcgcagtacc tcgctatgcg cggatatcga gtcttggcca ttgatctcga1681 tcctcaagcg agcctttctg cactctttgg gagccaaccg gagacggacg ttggcccgaa1741 cgaaacgctc tacggcgcta taaggtatga tgatgagcag gtggcaatcg aacgagtcgt1801 ccgagggact tacattcccg acctccacct gattcctggt aaccttgagc tgatggagtt1861 tgaacacgat acgccacgcg cgctgatgaa ccgcaaagag ggcgacacgc tcttttatgg1921 tcgcatcagc caagtaattg aagatatcgc ggataactat gacgtcgtgg tcatcgactg1981 ccctccccag cttgggtatc tcacgctatc cgcattgact gcggcgacgt ccattcttgt2041 cacggtccat ccgcagatgc tggatgtgat gtcgatgaac cagtttctgg caatgacatc2101 gaaccttttg cgtgaaatcg agaatgctgg cgccaagttc aagtttaatt ggatgcgcta2161 tctgataacc cgtttcgaac cgagcgacgg accacagaac caaatggtag gttatctgcg2221 gtcgattttt ggcgaaaatg tcctcaattt tccgatgctt aaaaccaccg cggtttcgga2281 cgctggcctg acaaaccaga ctctattcga agtggagcgt ggcctgttca cgcgctcgac2341 ctatgatcga gccttggagg cgatgaacgc cgtcaacgac gagatcgaaa cactgatcaa2401 aaaagcatgg ggtaggccca catgagccgg aagcacatcc ttggcgtctc aactgacgcc2461 cctgagacgt cgcccgccga caataggacg gcaaagaacc gctccatgcc gctcctcggc2521 gtaacaagga aggagcgcga tccggcaacg aagctcacag cgaacattgg taacgcactg2581 cgagagcaaa acgatcgtct tagccgtgcc gaagagatcg agcggcgtct cgctgaaggt2641 caggcagtga tagagttgga tgcctcgtca atagaaccgt ctttcgtgca ggatcgtatg2701 cgaggggaca ttgacgggct ccttacttcg atccgggaac aaggacagca agtcccaatc2761 cttgtgcgac cgcatccgag ccagccgggc cgatatcagg ttgccttcgg ccaccgccgg2821 ctacgcgccg tttcagaact cggacttccg gtcagggcgg tcgttcgcga actgacggac2881 gagcaagtgg tcgtagcaca gggtcaggaa aacaatgtgc gcgaagatct taccttcatc2941 gaaaaggcgc gcttcgcaca tcgcctgaac aggcagtttt ctcgagagat tgtcatcgcc3001 gcgatgtcga tcgacaagag caatttgtcc aagatgcttc tgctcgttga cgccctcccc3061 tctgaactga ccgatgctat tggtgccgct cctggtgttg gacggccgag ttggcaacaa3121 cttgccgagc tgattgagaa agtttcttca ccggccgacg tggctaaata tgctatgtcg3181 gaggaagttc aagcgctgcc atcggcagaa cgattcaagg cggtgatcgc tagtctgaag3241 cccagtcggg ttgcgcgtgg acttcccgag gtcatggcca ccccagacgg caccagaatt3301 gcacaggtga cgcagagcaa ggccaaactg gaaatcacga ttgacaggaa ggcgacgccc3361 gattttgcga ccttcgtgct cgatcatgtg ccagcgctgt atcaagcgta ccacgctgag3421 aaccaacgga aacggggaga gtaaaccgca aaagaaaaga gccccctcaa cgtcgccgtc3481 gcggaagccc ttctgtctct ctagcgcgaa cagaatcgca tttcctcgaa tcctcgtcaa3541 gagtttttag cgccgttttg gtgagctgat ttcctttgcc tgctgaaagg tgaaagatga3601 tgcagacagg aagtgtaacg acgccattcg ggcggcggcc aatgacgctt gcgcttgtgc3661 ggcgccagac ggcgctggcc gatatcaaac aaggcaagac agcggacaag tggaaggtct3721 ttagggacgc gtccgcggcc atggaactac ttggaatcca gtccaacagt cttgccgtcc3781 ttgatgcgct attgagcttt cacccggaaa cggagttgcg tcaggaggca cagctgatcg3841 tcttcccgtc gaatgctcag cttgcccttc gggcgcatgg gatggctggc gcgactttgc3901 gtaggcacat cgccatgctc gtggagtcag gcttgatcgt ccggaaggat agcgccaacg3961 gaaagcgtta cgctcgtaag gatggcgctg gtcagatcga gcgcgcgttt ggcttcgatt4021 tgtctccgct tctcgcgcgg tccgaagagc tagcgatgat ggcacagcag gtgatggccg4081 atcgagcagc attcaggatg gccaaagaaa gtctgacgat ttgccgacgg gacgttcgga4141 agctaattac ggcagctatg gaagagggag cggagggcga ctggcaagct gtcgaggaag4201 tctatgtgga acttgtgggt agaatcccac gcgccccgac gcttgctgat gtagagtcaa4261 ttctcgaaga gatgtggatg ctccaggaag agataatcaa ccggttggaa attagagaca4321 attcagaaaa taatagcacc aatgctgccc agagcgagca gcacatacag aactcaaaac4381 ccgaatccgt taatgaactt gaacctcgct ctgaaaagga gcagggcgct aagccgagtg4441 aaatagaccg ggcaaggagc gagccgataa aagcgttccc cctcgggatg atcctgaaag4501 cgtgcccgac cattggcaat tatgggccga gcggtgcggt tgctagctgg cgtgacctca4561 tgtcggctgc ggtggtggtt cggtctatgc tgggggtcag cccgtcggct taccaagacg4621 cgtgtgaggc aatgggaccg gagaatgcgg cagcagcgat ggcgtgcatt ttggagcgag4681 cgaacttcat caattcgcct gggggctatc tccgagatct gacacggcgg agcgaactcg4741 ggaagttttc acttggcccg atgataatgg cgctcttgaa ggctagcggg caggggacgt4801 tgcggtttgg ctagaattag cgagtatgga gcaggatggt ctgtggtcag ctgaccacag4861 acctaatagg ttgaaaacat gagcgttttt tggatgatcg acagaccatc cgattcccgg4921 agtaccaagc gtgctctgat gggagcgata acattactca acaagcacga aggccccatg4981 ccgatcgttg atcgtgaagg agagcctgct ctacatgcgg cggtattttg ccggccgagg5041 catgtagtcg cggagcactg cctatttact gccctaggca caaacgttga ctcttggatc5101 gagctggcag acaaagcaat aacccacaca gaggacgatt aatggctgac gaagagatcc5161 agaatccgcc ggacggtact gctgctgccg aagttgagcc ggctgctcct agaggtagaa5221 gagcaaagaa agcaccagcc gaaacagccc gcacgggatc gttcaaatcc gtgaagccga5281 aaacccgcgg cctcagcaac cgagaaaaac tggagaagat cggtcaaatc gaagctcagg5341 tcgctggcgg cgcaaccttg aaggacgccg ttaagatcgt gggtatttcc gttcagacct5401 attatcaatg gaagagagct gcggttcaac ctgtctcaca gaatccggcc gtgtctgttt5461 cagttgacga tgaactcggc gagttcatcc aactcgagga ggaaaatcgg cggctcagaa5521 agctagcttg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata5581 acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc5641 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg5701 gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt5761 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc5821 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg5881 gcccaaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg5941 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa6001 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat6061 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac6121 tttattgcca aatgtttgaa cgatcggggc ccgatcatat tgtcgctcag gatcgtggcg6181 ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac cttcgaccgc ctgttccgca6241 gagatcccgt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg6301 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa6361 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc6421 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac6481 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg6541 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc6601 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga6661 cgagatcatc gccgtcgggc atacgcgcct tgagcctggc gaacagttcg gctggcgcga6721 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac6781 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg6841 tatgcag
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