- 商品货号:FH2504
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 筛选标记:红霉素Erm
- 克隆菌株:大肠杆菌DH5α
- 表达宿主:枯草杆菌
- 质粒简介: pJMP1质粒是一个枯草杆菌基因编辑质粒。Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA。dcas9 was PCR-amplified from pdCas9-bacteria (Addgene #44249) using primers containing BamHI-compatible BsaI sites, digested with BsaI, then ligated into plasmid pAX01 digested with BamHI to generate pJMP1。
A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria.
Peters JM, Colavin A, Shi H, Czarny TL, Larson MH, Wong S, Hawkins JS, Lu CH, Koo BM, Marta E, Shiver AL, Whitehead EH, Weissman JS, Brown ED, Qi LS, Huang KC, Gross CA
Cell. 2016 Jun 2;165(6):1493-506. doi: 10.1016/j.cell.2016.05.003. Epub 2016 May 26. PubMed Journal- 质粒图谱1: jpg/9-1P514105Z0111.jpg
质粒序列: 点击查看序列LOCUS Exported 12080 bp ds-DNA circular SYN 14-MAY-2018DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..12080/organism="synthetic DNA construct"/mol_type="other DNA"CDS 1087..1278/codon_start=1/gene="rop"/product="Rop protein, which maintains plasmids at low copynumber"/note="rop"/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDGENL"misc_feature 1380..1520/note="bom"/note="basis of mobility region from pBR322"rep_origin complement(1706..2294)/direction=LEFT/note="ori"/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(2465..3325)/codon_start=1/gene="bla"/product="beta-lactamase"/note="AmpR"/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(3326..3430)/gene="bla"/note="AmpR promoter"CDS complement(3541..3948)/codon_start=1/note="'lacA"/translation="MKDYATVIDVKTASVEAVYQEDFYARTPAVTSHEYQQGKAYFIGARLEDQFQRDFYEGLITDLSLSPVFPVRHGKGVSVQARQDQDNDYIFVMNFTEEKQLVTFDQSVKDIMTGDILSGDLTMEKYEVRIVVNTH"terminator 4074..4108/note="lambda t0 terminator"/note="minimal transcription terminator from phage lambda(Scholtissek and Grosse, 1987)"CDS complement(4140..5264)/codon_start=1/note="bme xylR"/translation="MNQKLILDEILKNSPVSRATLSEITGLNKSTVSSQVNTLLEKDFIFEIGAGQSRGGRRPVMLVFNKNAGYSIGIDIGVDYLNGILTDLEGNIILEKTSDLSSSSASEVKEILFALIHGFVTHMPESPYGLVGIGICVPGLVDRHQQIIFMPNLNWNIKDLQFLIESEFNVPVFVENEANAGAYGEKVFGMTKNYENIVYISINIGIGTGLVINNELYKGVQGFSGEMGHMTIDFNGPKCSCGNRGCWELYASEKALLASLSKEEKNISRKEIVERANKNDVEMLNALQNFGFYIGIGLTNILNTFDIEAVILRNHIIESHPIVLNTIKNEVSSRVHSHLDNKCELLPSSLGKNAPALGAVSIVIDSFLSVTPIS"CDS 5580..9686/codon_start=1/product="catalytically dead mutant of the Cas9endonuclease from the Streptococcus pyogenes Type IICRISPR/Cas system"/note="dCas9"/note="RNA-guided DNA-binding protein that lacksendonuclease activity due to the D10A mutation in the RuvCcatalytic domain and the H840A mutation in the HNHcatalytic domain"/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"terminator 9710..9781/note="rrnB T1 terminator"/note="transcription terminator T1 from the E. coli rrnBgene"terminator 9797..9824/note="T7Te terminator"/note="phage T7 early transcription terminator"promoter complement(9899..10003)/gene="bla"/note="AmpR promoter"CDS 10382..11119/codon_start=1/note="Erm"/translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGRK"terminator 11289..11375/gene="Escherichia coli rrnB"/note="rrnB T1 terminator"/note="transcription terminator T1 from the E. coli rrnBgene"terminator 11394..11421/note="rrnB T2 terminator"/note="transcription terminator T2 from the E. coli rrnBgene"terminator 11501..11535/note="lambda t0 terminator"/note="minimal transcription terminator from phage lambda(Scholtissek and Grosse, 1987)"CDS complement(11559..12074)/codon_start=1/note="lacA'"/translation="MSKLEKTHVTKAKFMLHGGDYNPDQWLDRPDILADDIKLMKLSHTNTFSVGIFAWSALEPEEGVYQFEWLDDIFERIHSIGGRVILATPSGARPAWLSQTYPEVLRVNASRVKQLHGGRHNHCLTSKVYREKTRHINRLLAERYGHHPALLMWHISNEYGGDCHCESMRPL"ORIGIN1 atcctgatat tgtctgcatt tgcgccggag caaaccaaaa acctggtgag acacgccttg61 aattagtaga aaagaacttg aagattttca aaggcatcgt tagtgaagtc atggcgagcg121 gatttgacgg cattttctta gtcgcgacgc gaggctggat ggccttcccc attatgattc181 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag241 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga301 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt361 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg421 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac481 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc541 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg601 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct661 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg721 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt781 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat服务时间:24小时在线客服
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