- 商品货号:FH1269
- 规格:10μl质粒
- 启动子:GAL1,GAL10 promoter
- 质粒大小:6706bp
- 筛选标记:DH5α
- 克隆菌株:37℃,有氧,LB
- 质粒简介: The pESC vectors are a series of epitope-tagging vectors for expression of eukaryotic genes in the yeast S. cerevisiae. Each vector contains GAL1 and GAL10 yeast promoters in opposing orientations. With these vectors you can introduce one or two cloned genes into a yeast host strain under the control of an inducible promoter. These vectors also feature an extensive polylinker sequence and the ability to generate end-specific RNA transcripts from T3 and T7 promoters. Each of the pESC vectors contains one of four different yeast-selectable markers (HIS3, TRP1, LEU2, or URA3) in the same vector backbone. The pESC vectors contain DNA sequences coding for epitope peptides that can be specifically recognized by monoclonal antibodies. A sequence for the FLAG® epitope (DYKDDDDK) is located in the multiple cloning site (MCS) downstream of the GAL10 promoter; a sequence for the c-Myc epitope (EQKLISEEDL) is located in the MCS downstream of the GAL1 promoter. You can insert your gene of interest in front of the epitope sequence to generate C-terminal tagging or after the epitope sequence for N-terminal tagging. These tags allow the protein of interest to be studied without generating a specific antibody to that protein. The epitope-tagged fusion proteins can be studied in transformed cells using well-characterized antibodies. • Express two different genes simultaneously in S. cerevisiae
• Proteins tagged with unique epitope tags
• Fast and easy immunoprecipitations- 质粒图谱1: png/1-1FR31A626260.png
质粒序列: 点击查看序列LOCUS Exported 6706 bp ds-DNA circular SYN 13-APR-2017DEFINITION synthetic circular DNAACCESSION .VERSION .KEYWORDS pESC-HISSOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 6706)AUTHORS .TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..6706/organism="synthetic DNA construct"/mol_type="other DNA"promoter 317..503/gene="S. cerevisiae HIS3"/note="HIS3 promoter"CDS 504..1163/codon_start=1/gene="S. cerevisiae HIS3"/product="imidazoleglycerol-phosphate dehydratase, requiredfor histidine biosynthesis"/note="HIS3"/note="yeast auxotrophic marker"/translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAEQATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAFKEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFLESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"rep_origin complement(1423..1878)/direction=LEFT/note="f1 ori"/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"terminator 1961..2126/gene="S. cerevisiae ADH1"/note="ADH1 terminator"/note="transcription terminator for the S. cerevisiaealcohol dehydrogenase 1 (ADH1) gene"CDS complement(2274..2297)/codon_start=1/product="FLAG(R) epitope tag, followed by an enterokinasecleavage site"/note="FLAG"/translation="DYKDDDDK"promoter 2344..3008/gene="GAL1 and GAL10 genes of S. cerevisiae"/note="GAL1,10 promoter"/note="divergent inducible promoter, regulated by Gal4"protein_bind 2560..2677/bound_moiety="Gal4"/note="UAS"/note="upstream activating sequence mediatingGal4-dependent induction"promoter 3019..3037/note="T7 promoter"/note="promoter for bacteriophage T7 RNA polymerase"CDS 3055..3084/codon_start=1/product="Myc (human c-Myc oncogene) epitope tag"/note="Myc"/translation="EQKLISEEDL"terminator 3114..3303/gene="S. cerevisiae CYC1"/note="CYC1 terminator"/note="transcription terminator for CYC1"rep_origin complement(3546..4134)/direction=LEFT/note="ori"/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(4305..5165)/codon_start=1/gene="bla"/product="beta-lactamase"/note="AmpR"/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(5166..5270)/gene="bla"/note="AmpR promoter"rep_origin 5297..6639/note="2u ori"/note="yeast 2u plasmid origin of replication"protein_bind complement(6260..6307)/bound_moiety="FLP recombinase from the Saccharomycescerevisiae 2u plasmid"/note="FRT"/note="FLP-mediated recombination occurs in the 8-bp coresequence TCTAGAAA (Turan and Bode, 2011)."ORIGIN1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc1921 gatcggtgcg ggcctcttcg ctattacgcc agctgaattg gagcgacctc atgctatacc1981 tgagaaagca acctgaccta caggaaagag ttactcaaga ataagaattt tcgttttaaa2041 acctaagagt cactttaaaa tttgtataca cttatttttt ttataactta tttaataata2101 aaaatcataa atcataagaa attcgcttat ttagaagtgt caacaacgta tctaccaacg2161 atttgaccct tttccatctt ttcgtaaatt tctggcaagg tagacaagcc gacaaccttg2221 attggagact tgaccaaacc tctggcgaag aattgttaat taagagctca gatcttatcg2281 tcgtcatcct tgtaatccat cgatactagt gcggccgccc tttagtgagg gttgaattcg2341 aattttcaaa aattcttact ttttttttgg atggacgcaa agaagtttaa taatcatatt2401 acatggcatt accaccatat acatatccat atacatatcc atatctaatc ttacttatat2461 gttgtggaaa tgtaaagagc cccattatct tagcctaaaa aaaccttctc tttggaactt2521 tcagtaatac gcttaactgc tcattgctat attgaagtac ggattagaag ccgccgagcg2581 ggtgacagcc ctccgaagga agactctcct ccgtgcgtcc tcgtcttcac cggtcgcgtt2641 cctgaaacgc agatgtgcct cgcgccgcac tgctccgaac aataaagatt ctacaatact2701 agcttttatg gttatgaaga ggaaaaattg gcagtaacct ggccccacaa accttcaaat2761 gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag ccttatttct2821 ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat aaatgcaaaa2881 actgcataac cactttaact aatactttca acattttcgg tttgtattac ttcttattca2941 aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta acgtcaagga3001 gaaaaaaccc cggatccgta atacgactca ctatagggcc cgggcgtcga catggaacag3061 aagttgattt ccgaagaaga cctcgagtaa gcttggtacc gcggctagct aagatccgct3121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata3181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga3241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg3301 aagatccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg3361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg3421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
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