- 商品货号:QT101
- 规格:10μl质粒
- 启动子:Ac5 promoter
- 复制子:pUC ori
- 终止子:SV40 poly(A) signal
- 质粒分类:昆虫系列质粒;昆虫游离质粒;昆虫胞内质粒
- 质粒大小:6118bp
- 质粒标签:N-EGFP,C-V5,C-6×His
- 原核抗性:氨苄青霉素Amp
- 筛选标记:无
- 克隆菌株:DH5α等大肠杆菌
- 表达宿主:果蝇细胞系S2
- 诱导方式:无需诱导,组成型表达
- 5测序引物:Ac5-F(ACACAAAGCCGCTCCATCAG)
- 3测序引物:BGH-R(TAGAAGGCACAGTCGAGG)
- 备注:非病毒
- 质粒简介: pAC5.1b-EGFP-2质粒是一个昆虫细胞组成型表达载体,Ac5启动子驱动EGFP标签、目的基因、Myc标签和6×HIS标签一起融合表达,可以与筛选载体pCoHygro或者pCoBlast共转染,建立稳表达细胞系。本质粒由武汉淼灵生物科技有限公司共享至淼灵质粒平台。 pAC5.1b-EGFP② is a 6kb expression vector designed for use with the Drosophila Constitutive Expression System available from Invitrogen. Upon transfection, the vector allows transient expression of your protein of interest in Drosophila cells. When cotransfected with the selection vector, pCoHygro or pCoBlast, pAC5.1b-EGFP allows selection of stable cell lines exhibiting constitutive expression of the protein of interest. • The Drosophila actin 5C (Ac5) promoter for high-level, constitutive expression of the gene of interest in S2 cells (Chung and Keller, 1990).
• Multiple cloning site to facilitate cloning the gene of interest.
• C-terminal peptide containing the V5 epitope and polyhistidine (6xHis) tag for detection and purification of your protein of interest (if desired).
• Three reading frames to facilitate in-frame cloning with the C-terminal peptide.
• N-terminal EGFP tag for better detection.
• Ampicillin resistance gene for selection of transformants in E. coli.- 质粒图谱1: png/1-1FR50R503K9.png
质粒序列: 点击查看序列LOCUS Exported 6118 bp ds-DNA circular SYN 25-AUG-2017DEFINITION synthetic circular DNAKEYWORDS pAC5.1b-EGFP②SOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 6118)TITLE Direct SubmissionJOURNAL Exported Aug 25, 2017 from MiaoLingPlasmidhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..6118/organism="synthetic DNA construct"/mol_type="other DNA"promoter 56..2568/label=Ac5 promoter/note="Drosophila melanogaster actin 5C promoter"primer_bind 2525..2544/label=AC5-FCDS 2617..3333/codon_start=1/product="enhanced GFP"/label=EGFP/note="mammalian codon-optimized"/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"misc_feature 3336..3402/label=MCSCDS 3403..3444/codon_start=1/product="epitope tag from simian virus 5"/label=V5 tag/translation="GKPIPNPLLGLDST"CDS 3454..3471/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"polyA_signal 3544..3678/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"primer_bind complement(3622..3641)/label=Sv40-polyA-Rrep_origin complement(4300..4888)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(5059..5919)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(5920..6024)/gene="bla"/label=AmpR promoterORIGIN1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg2581 tacctactag tccagtgtgg tggaatttcg agctttatgg tgagcaaggg cgaggagctg2641 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc2701 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc2761 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc2821 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc2881 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag2941 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc3001 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc3061 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc3121 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc3181 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg3241 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc3301 gggatcactc tcggcatgga cgagctgtac aagccaagct tgaattctgc agatatccag3361 cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc tatccctaac3421 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa3481 acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc agcctcgatc3541 gaggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag3601 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata3661 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg3721 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat3781 gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg3841 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca3901 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat3961 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac4021 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga4081 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat4141 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca4201 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagccagca4261 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc4321 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata4381 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc4441 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc4501 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga4561 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc4621 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag4681 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag4741 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag4801 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca4861 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga4921 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat4981 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga5041 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg5101 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga5161 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc5221 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac5281 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc5341 agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc5401 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc服务时间:24小时在线客服
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