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| 发货规格 |
1×10⁶ |
| 别名 |
CFPac-1; CF PAC-1; CF-PAC1; CF-Pac1; CF Pac1; CFPAC1; CFPac1; CFPAC |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
IMDM+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
30~45h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
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| 种属 |
人源 |
| 组织来源 |
胰腺 |
| 肿瘤类型 |
囊性纤维化,胰腺导管腺癌 |
| 供体信息 |
26岁白人男性 |
| 背景描述 |
CFPAC-1 细胞来源于一名 26 岁男性,患有囊性纤维化和导管腺癌肝转移,是一种超二倍体细胞系,具有生物学研究的显着特征。它们在裸鼠中的粘附性、生长特性和致瘤能力使它们成为体外癌症研究的实用模型。该细胞系的核型包括 73 条染色体的模态数,具有多个易位,重要的是,囊性纤维化基因所在的 7 号染色体的两到三个拷贝。 |
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| 基因表达 |
carcinoembryonic antigen (CEA) 9 ng/mL per 106 cells per 10 days; pancreatic oncofetal antigen (POA) 28 ng/mL; adenocarcinoma associated antigen (ACAA) 5000 ng/mL; CA 19-9 antigen 12000 units/mL; epithelial keratins |
| 致瘤性 |
Yes, in nude mice (passage 34) |
| 抗原表达 |
CA19-9 antigen, 12000 units/mL, epithelial keratins |
| 核型 |
hyperdiploid; modal number = 73; range = 65 to 75; several translocations were evident; two to three apparently normal copies of number 7 (to which the cystic fibrosis gene has been mapped) were present in every metaphase examined |