细胞信息
发货规格	1×10⁶
别名	HBL 100; HBL100
形态	上皮细胞样
生长特性	贴壁
培养条件	DMEM+10%FBS+1%P/S
冻存条件	90%FBS+10%DMSO
传代方式	胰酶消化
倍增时间	28~40h
传代比例	1:02
换液频率	每周2~3次
生物安全等级	2
细胞背景
种属	人源
组织来源	乳腺
供体信息	27岁，女性，白种人
背景描述	该细胞由E.V.Gaffney及其同事从一位没有乳癌家族史的供者乳汁中建立，培养出来的细胞染色体组型在第7代时就不正常；电镜照片显示有微丝、张力原纤维和桥粒；Southern转移表明有整合型SV40病毒基因，不可以当作正常细胞。
遗传背景
同工酶	G6PD， B， PGM1， 1， PGM3， 2， ES-D， 1， Me-2， 0， GLO-1， 2， AK-1， 1-2， Phenotype Frequency Product: 0.0008
致瘤性	裸鼠中可成瘤
抗原表达	HLA A1， A10， A11， B7， B8
病毒	The cells contain a tamdemly integrated SV40 genome it has been reported that they may contain a type D retrovirus that is similar or identical to Mason-Pfizer monkey virus (MPMV).
逆转录酶	阳性
核型	The stemline chromosome number is near triploid with the modal number of 67 chromosomes， and the 2S component occurring at 0.6%. Most chromosome complements consist of about 39 normal and 28 marker chromosomes. Markers such as 2q， 11q+， 11q， t(2q.12)， t(2q.5q?)， t(6p?.16)， 16pt and many others are common to most metaphases. Normal chromosomes 11， 14， 15 and 16 are absent. 2， 12， 17 and 19 are monosomic， and the x is disomic. DNA profiling for amelogenin， a sex-chromosome-specific PCR assay that can distinguish x chromosome-specific products from Y chromosome-specific products revealed the presence of Y chromosomes in this cell line of putative female origin. Confirmation of the general findings was accomplished by QM staining， C-banding， and FISH， with a whole chromosome paint probe to the human Y chromosome.
倍体	非整倍体

STR鉴定

STR位点信息

STR鉴定图

Amelogenin	X (CCRID; PubMed=25877200; PubMed=28889351) X,Y (ATCC=HTB-124; CLS=300178; PubMed=11416159)
CSF1PO	10
D2S1338	18,24
D3S1358	14,16
D5S818	11,12
D7S820	8,12
D8S1179	12,15
D13S317	12
D16S539	9,12
D18S51	16
D19S433	15
D21S11	28,30
FGA	25
Penta D	12
Penta E	7
TH01	6,8
TPOX	8
vWA	16