• Source： Chinese Hamster Ovary cell line
• Description： Human Vitronectin (Asp20-Leu478) Accession #P04004
• Predicted molecular mass： 52.3kDa
• Purity： ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
• Endotoxin： ≤1 EU/mg by the LAL method
• Activity： Measured by the ability of the immobilized protein to support the adhesion of B16-F1 mouse melanoma cells. The ED50 for this effect is 2-10 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.
• Formulation： Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
• Appearance： White powder, Colorless clear liquid after reconstitution
• Reconstitution： It is recommended to redissolve in sterile deionized water.
• Shipping： The product is shipped with blue ice.
• Storage & Stability： 24 months at -20°C to -80°C in lyophilized state 7-10 days at 2°C to 8°C under sterile conditions after reconstitution 6 months at -20°C to -80°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles.