• Source： E.coil
• Description： Bovine bFGF (Pro10-Ser155), with a C-terminal 6-His tag Accession # P03969
• Predicted molecular mass： 17.4 kDa
• Purity： ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
• Endotoxin： ≤100 EU/mg by the LAL method
• Activity： Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is ≤2.0 ng/mL.
• Formulation： Lyophilized from a 0.22 μm-filtered solution containing 10mM Tris,200mM Nacl, 5% mannitol, pH 8.0.
• Appearance： White powder, Colorless clear liquid after reconstitution
• Reconstitution： It is recommended to redissolve in sterile deionized water.
• Shipping： The product is shipped with blue ice.
• Storage & Stability： 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles.