• Source： Chinese Hamster Ovary cell line
• Description： Recombinant human IL-12 is composed of P40(Ile23-Ser328) and P35(Arg23-Ser219) subunits,which linked by GS-linker （GSGSSRGGSGSGGSGGGGSK）Accession # P29460(IL12P40), P29459(IL12P35)
• Predicted molecular mass： 59 kDa
• Purity： ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
• Endotoxin： ≤10 EU/mg by the LAL method
• Activity： Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5 ng/mL.
• Formulation： Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
• Appearance： White powder, Colorless clear liquid after reconstitution
• Reconstitution： It is recommended to redissolve in sterile deionized water.
• Shipping： The product is shipped with blue ice.
• Storage & Stability： 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution Use a manual defrost freezer and avoid repeated freeze-thaw cycles.