- 细胞信息
发货规格 1×10⁶ 别名 HL 60; HL.60; HL60; Human Leukemia-60 形态 淋巴母细胞样 生长特性 悬浮 培养条件 IMDM+20%FBS+1%P/S 冻存条件 90%FBS+10%DMSO 传代方式 直接传代 传代比例 维持细胞浓度在1x105~1x106个/ml 换液频率 每周2~3次
- 细胞背景
种属 人源 组织来源 血液 肿瘤类型 急性早幼粒细胞白血病 供体信息 36岁白人女性 背景描述 HL-60 细胞来源于一名患有急性早幼粒细胞白血病的 36 岁女性,由于它们能够分化为成熟的白细胞并模拟先天免疫反应,因此能够分化为成熟的白细胞并模拟先天免疫反应,有助于理解白血病进展、细胞癌基因表达和治疗靶点识别
- 遗传背景
同工酶 AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1 PGM3, 1 基因表达 myc+ 致癌基因 Yes, in nude mice 抗原表达 Complement, expressed; Fc, expressed 核型 The stemline chromosome number is pseudodiploid with the 2S component occurring at 6.2%. Five markers (M2 through M6) were common to most S metaphases. DM’s, which varied in numbers per cell, occurred in all metaphases karyotyped. HSR chromosomes were not detected. STR鉴定STR位点信息STR鉴定图
Amelogenin X CSF1PO 13,14 D2S1338 17 D3S1358 16 D5S818 12 D7S820 11,12 D8S1179 12 (DSMZ=ACC-3) 12,13 (ATCC=CCL-240; CCRID; PubMed=11416159; PubMed=25877200) 13 (CLS=300209) D13S317 8,11 D16S539 11 D18S51 14 (CLS=300209) 14,15 (ATCC=CCL-240; CCRID; DSMZ=ACC-3; PubMed=11416159; PubMed=25877200) D19S433 14 D21S11 29,30 (ATCC=CCL-240; CCRID; COG; DSMZ=ACC-3; PubMed=25877200) 30 (CLS=300209; PubMed=11416159) FGA 22,24 Penta D 10,11 (DSMZ=ACC-3) 10,12 (ATCC=CCL-240; CLS=300209; PubMed=25877200) Penta E 13 (CLS=300209) 13,14 (ATCC=CCL-240; DSMZ=ACC-3; PubMed=25877200) TH01 7,8 (AddexBio=C0003023/4977; ATCC=CCL-240; CCRID; CLS=300209; COG; Cosmic-CLP=905938; DSMZ=ACC-3; JCRB=JCRB0085; KCLB=10240; PubMed=11416159; PubMed=25877200; RCB=RCB0041; TKG=TKG 0345) 8 (ECACC=98070106; JCRB=IFO50022) TPOX 8,11 vWA 16