MOLT-4细胞（人急性淋巴母细胞性白血病细胞）

商品货号：CL0224

￥1500元

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细胞信息

发货规格 1×10⁶
别名 Molt-4;MOLT4;Molt4;MOLT.4;MOLT4;Molt4;GM02219;GM02219C;GM2219C;GM02219D
形态淋巴母细胞样
生长特性悬浮
培养条件 RPMI-1640+10%FBS+1%P/S
冻存条件 90%FBS+10%DMSO
传代方式离心
倍增时间 30~40h
传代比例维持细胞浓度在1x105~1x106个/ml
换液频率每周2~3次
生物安全等级 1

细胞背景

种属人源
组织来源外周血
肿瘤类型成人 T 急性淋巴细胞白血病
供体信息 19岁白人男性
背景描述 MOLT-4是一种T淋巴细胞系，来源于1971年复发的一名19岁急性淋巴细胞白血病（ALL）男性患者的外周血。它是MOLT-3的姊妹细胞系，而MOLT-4显示出不寻常的T细胞抗原受体γ链基因（T-γ）重排。MOLT-4细胞的倍增时间约为30小时，悬浮生长，并且在未经治疗的裸鼠、抗淋巴细胞血清处理的小鼠和X辐照小鼠中具有致瘤性。

遗传背景

蛋白表达 p53 positive
基因表达 High levels of terminal deoxynucleotidyl transferase (TdT) are produced
致瘤性 Yes， in untreated nude mice， anti lymphocyte serum treated mice and X-irradiated mice
抗原表达 CD1 (49%)， CD2 (35%)， CD3 A (26%) B (33%) C (34%)， CD4 (55%)， CD5 (72%)， CD6 (22%)， CD7 (77%)
病毒 The cells do not produce immunoglobulin or Epstein-Barr virus (Minowada， 1972).
突变信息 G -> A mutation at codon 248 of the p53 gene， P53 is not expressed (Rodrigues， 1990).
核型 Hypertetraploid. Modal number: 96. Two x and two Y chromosomes.

STR鉴定

STR位点信息

STR鉴定图

Amelogenin X,Y
CSF1PO 11,12 (CLS=300115; DSMZ=ACC-362) 11,12,13 (ATCC=CRL-1582; CCRID; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D2S1338 23,24
D3S1358 14,16 (ATCC=CRL-1582; CCRID; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200) 15,16 (CLS=300115; DSMZ=ACC-362)
D5S818 11,12 (DSMZ=ACC-362) 12 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 12,13 (PubMed=25877200)
D7S820 8,10 (DSMZ=ACC-362) 8,10,11 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D8S1179 9,13,14 (ATCC=CRL-1582; CLS=300115; DSMZ=ACC-362) 9,13,14,15 (PubMed=25877200) 9,14 (CCRID; COG; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 9,14,15 (PubMed=11416159)
D13S317 12,13 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200) 12,13,14 (DSMZ=ACC-362)
D16S539 11,14 (ATCC=CRL-1582; CCRID; CLS=300115; Cosmic-CLP=905958; DSMZ=ACC-362; JCRB=IFO50362; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 11,14,15 (COG; JCRB=JCRB9031; PubMed=25877200)
D18S51 12,13,16,17,18 (DSMZ=ACC-362) 12,13,17 (CLS=300115) 13,16,17 (ATCC=CRL-1582; DOI=10.4172/2157-7145.S2-005) 13,17 (CCRID; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D19S433 14,15 (ATCC=CRL-1582; CCRID; DOI=10.4172/2157-7145.S2-005; DSMZ=ACC-362; PubMed=20922763; PubMed=25877200) 14,15,16 (PubMed=19372543)
D21S11 27,28,29,30 (ATCC=CRL-1582) 27,28,29,30,31 (COG; DOI=10.4172/2157-7145.S2-005; PubMed=25877200) 28,29,30 (CLS=300115; DSMZ=ACC-362; PubMed=11416159) 28,29,30,31 (CCRID; PubMed=19372543; PubMed=20922763)
FGA 21,22,23,24,25 (PubMed=25877200) 21,22,24,25 (DSMZ=ACC-362) 22,23,24,25 (ATCC=CRL-1582; COG; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 22,23,25 (CCRID) 22,24 (CLS=300115) 22,25 (PubMed=11416159)
Penta D 8,12,13 (CLS=300115; DSMZ=ACC-362) 8,13 (ATCC=CRL-1582; PubMed=25877200)
Penta E 14,15 (DSMZ=ACC-362) 14,15,16 (CLS=300115) 14,16 (ATCC=CRL-1582; PubMed=25877200)
TH01 6,8
TPOX 8
vWA 16,17,18 (DSMZ=ACC-362) 17 (PubMed=11416159) 17,18 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=20922763; PubMed=25877200) 17,18,19 (PubMed=19372543)

细胞信息
发货规格	1×10⁶
别名	Molt-4;MOLT4;Molt4;MOLT.4;MOLT4;Molt4;GM02219;GM02219C;GM2219C;GM02219D
形态	淋巴母细胞样
生长特性	悬浮
培养条件	RPMI-1640+10%FBS+1%P/S
冻存条件	90%FBS+10%DMSO
传代方式	离心
倍增时间	30~40h
传代比例	维持细胞浓度在1x105~1x106个/ml
换液频率	每周2~3次
生物安全等级	1
细胞背景
种属	人源
组织来源	外周血
肿瘤类型	成人 T 急性淋巴细胞白血病
供体信息	19岁白人男性
背景描述	MOLT-4是一种T淋巴细胞系，来源于1971年复发的一名19岁急性淋巴细胞白血病（ALL）男性患者的外周血。它是MOLT-3的姊妹细胞系，而MOLT-4显示出不寻常的T细胞抗原受体γ链基因（T-γ）重排。MOLT-4细胞的倍增时间约为30小时，悬浮生长，并且在未经治疗的裸鼠、抗淋巴细胞血清处理的小鼠和X辐照小鼠中具有致瘤性。
遗传背景
蛋白表达	p53 positive
基因表达	High levels of terminal deoxynucleotidyl transferase (TdT) are produced
致瘤性	Yes， in untreated nude mice， anti lymphocyte serum treated mice and X-irradiated mice
抗原表达	CD1 (49%)， CD2 (35%)， CD3 A (26%) B (33%) C (34%)， CD4 (55%)， CD5 (72%)， CD6 (22%)， CD7 (77%)
病毒	The cells do not produce immunoglobulin or Epstein-Barr virus (Minowada， 1972).
突变信息	G -> A mutation at codon 248 of the p53 gene， P53 is not expressed (Rodrigues， 1990).
核型	Hypertetraploid. Modal number: 96. Two x and two Y chromosomes.

Amelogenin	X,Y
CSF1PO	11,12 (CLS=300115; DSMZ=ACC-362) 11,12,13 (ATCC=CRL-1582; CCRID; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D2S1338	23,24
D3S1358	14,16 (ATCC=CRL-1582; CCRID; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200) 15,16 (CLS=300115; DSMZ=ACC-362)
D5S818	11,12 (DSMZ=ACC-362) 12 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 12,13 (PubMed=25877200)
D7S820	8,10 (DSMZ=ACC-362) 8,10,11 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D8S1179	9,13,14 (ATCC=CRL-1582; CLS=300115; DSMZ=ACC-362) 9,13,14,15 (PubMed=25877200) 9,14 (CCRID; COG; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 9,14,15 (PubMed=11416159)
D13S317	12,13 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763; PubMed=25877200) 12,13,14 (DSMZ=ACC-362)
D16S539	11,14 (ATCC=CRL-1582; CCRID; CLS=300115; Cosmic-CLP=905958; DSMZ=ACC-362; JCRB=IFO50362; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 11,14,15 (COG; JCRB=JCRB9031; PubMed=25877200)
D18S51	12,13,16,17,18 (DSMZ=ACC-362) 12,13,17 (CLS=300115) 13,16,17 (ATCC=CRL-1582; DOI=10.4172/2157-7145.S2-005) 13,17 (CCRID; PubMed=19372543; PubMed=20922763; PubMed=25877200)
D19S433	14,15 (ATCC=CRL-1582; CCRID; DOI=10.4172/2157-7145.S2-005; DSMZ=ACC-362; PubMed=20922763; PubMed=25877200) 14,15,16 (PubMed=19372543)
D21S11	27,28,29,30 (ATCC=CRL-1582) 27,28,29,30,31 (COG; DOI=10.4172/2157-7145.S2-005; PubMed=25877200) 28,29,30 (CLS=300115; DSMZ=ACC-362; PubMed=11416159) 28,29,30,31 (CCRID; PubMed=19372543; PubMed=20922763)
FGA	21,22,23,24,25 (PubMed=25877200) 21,22,24,25 (DSMZ=ACC-362) 22,23,24,25 (ATCC=CRL-1582; COG; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=19372543; PubMed=20922763) 22,23,25 (CCRID) 22,24 (CLS=300115) 22,25 (PubMed=11416159)
Penta D	8,12,13 (CLS=300115; DSMZ=ACC-362) 8,13 (ATCC=CRL-1582; PubMed=25877200)
Penta E	14,15 (DSMZ=ACC-362) 14,15,16 (CLS=300115) 14,16 (ATCC=CRL-1582; PubMed=25877200)
TH01	6,8
TPOX	8
vWA	16,17,18 (DSMZ=ACC-362) 17 (PubMed=11416159) 17,18 (ATCC=CRL-1582; CCRID; CLS=300115; COG; Cosmic-CLP=905958; JCRB=IFO50362; JCRB=JCRB9031; KCLB=21582; DOI=10.4172/2157-7145.S2-005; PubMed=20922763; PubMed=25877200) 17,18,19 (PubMed=19372543)

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