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| 发货规格 |
1×10⁶ |
| 别名 |
RT4; RT4P |
| 形态 |
上皮细胞样 |
| 生长特性 |
贴壁 |
| 培养条件 |
McCOY’s5A+10%FBS+1%P/S |
| 冻存条件 |
90%FBS+10%DMSO |
| 传代方式 |
胰酶消化 |
| 倍增时间 |
66~80h |
| 传代比例 |
1:2~1:4 |
| 换液频率 |
每周2~3次 |
| 生物安全等级 |
1 |
|
|
|
| 种属 |
人源 |
| 组织来源 |
膀胱 |
| 肿瘤类型 |
移行细胞状瘤 |
| 供体信息 |
63岁白人男性 |
| 背景描述 |
1968 年，一名 63 岁男性的复发性高分化移行性膀胱移行状肿瘤（临床分期 T2，组织学分级 G1，2 年前用金颗粒治疗，10 个月后复发后透热治疗） |
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|
|
| 蛋白表达 |
p53 positive |
| 同工酶 |
AK-1， 1；ES-D， 1-2；G6PD， B；GLO-I， 1-2；Me-2， 1；PGM1， 1-2；PGM3， 1-2 |
| 致瘤性 |
Yes， in cheek pouch of steroid-treated hamsters |
| 抗原表达 |
HLA A25(10)， A3， B12， Cw3; Blood Type O |
| 核型 |
modal number = 49; range = 42 to 55. The cell line is aneuploid human male， with chromosome counts in the near-diploid range. However， the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21)， del(10)(p11)， 14p+， 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy metaphases. |
