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UM-UC-3细胞(人膀胱移行细胞癌)
UM-UC-3细胞(人膀胱移行细胞癌)
  • 商品货号:CL0342
  • ¥1500元
    已售 19 件 | 评价 0 人次 | 关注度 1199
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    • 细胞信息
     
    发货规格 1×10⁶
    别名 UMUC-3; UM-UC3; UMUC3; UC-3; University of Michigan-Urothelial Carcinoma-3
    形态 上皮细胞样
    生长特性 贴壁
    培养条件 DMEM+10%FBS+1%P/S
    冻存条件 90%FBS+10%DMSO
    传代方式 胰酶消化
    倍增时间 24h
    传代比例 1:2~1:4
    换液频率 每周2~3次
    生物安全等级 1
    • 细胞背景
     
    种属 人源
    组织来源 膀胱
    肿瘤类型 膀胱癌
    供体信息 男性欧洲人
    背景描述 UM-UC-3 [UMUC3] 是一种上皮样细胞,是从患者的膀胱男性中分离出来的,可用于癌症研究。
    • 遗传背景
     
    致瘤性 yes,Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
    核型 This is a hypertriploid human cell line. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t(1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two to three copies per cell.
    STR鉴定
    STR位点信息
    STR鉴定图
    Amelogenin X
    CSF1PO 10 (ECACC=96020936; PubMed=25877200; PubMed=27270441) 10,11 (ATCC=CRL-1749; Cosmic-CLP=724838)
    D2S1338 23
    D3S1358 17,18
    D5S818 12
    D7S820 8,9 (ATCC=CRL-1749; Cosmic-CLP=724838; ECACC=96020936; PubMed=25877200) 9 (PubMed=27270441)
    D8S1179 13
    D13S317 8
    D16S539 8,9
    D18S51 14
    D19S433 14,15
    D21S11 31
    FGA 20,21
    Penta D 13
    Penta E 12
    TH01 6,9
    TPOX 10
    vWA 17