细胞信息
发货规格	1×10⁶
别名	CAKI-1; CaKi-1; caki-1; CAKI.1; CAKI 1; CAKI1; Caki1
形态	上皮细胞样
生长特性	贴壁
培养条件	McCoy’s5A+10%FBS+1%P/S
冻存条件	90%FBS+10%DMSO
传代方式	胰酶消化
倍增时间	40~60h
传代比例	1:2~1:4
换液频率	每周2~3次
生物安全等级	1
细胞背景
种属	人源
组织来源	肾
肿瘤类型	透明细胞癌
供体信息	49岁白人男性
背景描述	Caki-1 细胞系来源于人肾透明细胞癌的转移部位。Caki-1 细胞由位于男性患者肾静脉壁的肿瘤建立而成，通常用于肾癌生物学研究，特别是在了解透明细胞肾细胞癌 （ccRCC） 的潜在机制方面。该细胞系在形态上呈上皮样，并表现出强大的体外生长特性，使其适用于各种实验技术，包括药物筛选和分子生物学研究。
遗传背景
致瘤性	Yes， in nude mice
抗原表达	Blood Type O; Rh-; HLA A9， B12， Bw35
核型	modal number = 68; range = 63 to 71.The cell line is aneuploid human， with chromosome counts in the triploid range. The Y chromosome is absent; however， loss of the Y chromosome is not unusual from male tumor cell lines. All normal autosomes except chromosome N9 and N19 are represented， usually by two or three copies. Chromosome N9 is recognized as a marker chromosome (M1) that is usually trisomic. Normal chromosome N5 is and chromosomes N10 and N16 tend to be over-represented with respect to the copy number of other normal chromosomes. Thirteen marker chromosomes are identified: 9q+， t(1p;?)， t(1qter>1q21::20)， t(1q17q)， der(11)t(3;11)(q21;q14)， 19q+， 4q+， 4p+ and others. The chromosome counts and general cytogenetic features are in keeping with those described by J. Fogh， et al.， J. Natl. Cancer Inst. (Bethesda) 58: 209， 1977.

STR鉴定

STR位点信息

STR鉴定图

Amelogenin	X
CSF1PO	10,11
D2S1338	17 (PubMed=19372543) 17,19 (ATCC=HTB-46; CCRID; DSMZ=ACC-731)
D3S1358	17
D5S818	11,12
D7S820	8,12 (ATCC=HTB-46; CCRID; CLS=300149; Cosmic-CLP=905963; DSMZ=ACC-731; JCRB=JCRB0801; KCLB=30046; PubMed=25877200; RCB=RCB1985) 12 (PubMed=19372543)
D8S1179	12,14
D13S317	11 (DSMZ=ACC-731) 11,12 (ATCC=HTB-46; CCRID; CLS=300149; Cosmic-CLP=905963; JCRB=JCRB0801; KCLB=30046; PubMed=19372543; PubMed=25877200; RCB=RCB1985)
D16S539	12
D18S51	14
D19S433	14
D21S11	28,30
FGA	26
Penta D	11,12
Penta E	11,22,23 (ATCC=HTB-46) 22,23 (CLS=300149; DSMZ=ACC-731; PubMed=25877200)
TH01	6,8
TPOX	8,11
vWA	15,17