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发货规格 |
1×10⁶ |
别名 |
MDA-MB 361; MDA MB 361; MDA-MB361; MDAMB361; MDA-361; MDA361; MB361; MD Anderson-Metastatic Breast-361 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
L-15+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
93~103h |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
胸;乳腺 |
肿瘤类型 |
乳腺癌 |
背景描述 |
该细胞系起源于一名患有乳腺癌的 40 岁白人女性,但这些细胞是从大脑的转移部位分离出来的。该细胞系可用于癌症和神经科学研究。 |
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同工酶 |
AK-1, 1;ES-D, 1;G6PD, B;GLO-I, 2;Me-2, 1;PGM1, 1;PGM3, 1-2 |
基因表达 |
wnt7h+ |
致癌基因 |
wnt7h+ |
逆转录酶 |
阴性 |
核型 |
modal number. = 56; range = 54 to 61.The cell line is aneuploid human female, with chromosome counts in the hyperdiploid range. Normal chromosomes N11 and N17 are absent, chromosomes N1, N20, and N21 are weakly represented, and chromosomes N2, N8, N9, and N15 are single. The remainder of chromosomes are often paired. Eighteen marker chromosomes are found, of which 10 are consistently present. Some of these markers are found to be quite comparable to those described by K.L. Satya-Prakash, et al., in their report on this cell line. |