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发货规格 |
1×10⁶ |
别名 |
H292; H-292; NCI-HUT-292; Hut292; NCIH292 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
RPMI-1640+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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种属 |
人源 |
组织来源 |
肺 |
肿瘤类型 |
肺黏膜表皮样癌 |
供体信息 |
32岁女性非洲人 |
背景描述 |
细胞在培养物中保留其粘液表皮样特征,这是由它们的超微结构和多种鳞状分化标志物的表达决定的。这些细胞支持乙型肝炎病毒的生长,并且对 L-DOPA 脱羧酶呈阴性。细胞角蛋白和波形蛋白染色呈阳性,粘液胭脂红呈阳性,但神经丝三联体蛋白呈阴性。 |
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蛋白表达 |
Keratin, Vimentin, The Cells Retain Their Mucoepidermoid Characteristics In Culture As Determined By Their Ultrastructure And Expression Of Multiple Markers Of Squamous Differentiation. Tank Cells Show Reactivity With Human Alloantisera Specific For The H |
基因表达 |
keratin; vimentin; expression of multiple markers of squamous differentiation; reactivity with human alloantisera specific for the HLA: (A1; A3; A10; A19; B12; B17; B22; and B40 cross-reactive groups) |
致瘤性 |
Yes, in nude mice; tumors histologically resemble the original biopsy specimen and retain mucoepidermoid features |
病毒易感性 |
Hepatitis B virus |