|
|
发货规格 |
1×10⁶ |
别名 |
Jeg-3; JEG3; Jeg3; jeg3 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
MEM+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
24~36h |
传代比例 |
1:2~1:4 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
|
|
种属 |
人源 |
组织来源 |
胎盘 |
肿瘤类型 |
绒毛膜癌 |
背景描述 |
这是Kohler及其同事从Erwin-Turner肿瘤的Woods细胞中分离出的六个克隆衍生品系之一。细胞能够将类固醇前体转化为雌酮和雌二醇。 |
|
|
蛋白表达 |
hCG, human chorionic somatomammotrophin (placental lactogen), progesterone. |
同工酶 |
AK-1, 1;ES-D, 1;G6PD, B;GLO-I, 1-2;PGM1, 1;PGM3, 1-2 |
基因表达 |
estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/mL after reculturing |
致瘤性 |
Yes, in nude mice; forms malignant tumor consistent with choriocarcinoma |
核型 |
This is a hypertriploid human cell line. The modal chromosome number is 71, occurring at 34%, and polyploidy at 2.6%. The t(4;11)(p15;q13), i(13q), t(10p15q), del(18)(q21), and 6 other markers are common to most cells, and two other markers are found in some. Giant satellites are seen in one N14, and two N22. N2, N5, and N9 have 4 copies, and N7, N13, N18, N21 and X a single copy. A single Y chromosome is detected by Q-band examination. |