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发货规格 |
1×10⁶ |
别名 |
CaLu-6, CALU-6, Calu.6, Calu 6, Calu6, CALU6, CaLu-06 |
形态 |
上皮细胞样 |
生长特性 |
贴壁 |
培养条件 |
MEM+10%FBS+1%P/S |
冻存条件 |
90%FBS+10%DMSO |
传代方式 |
胰酶消化 |
倍增时间 |
26~72h |
传代比例 |
1:3~1:6 |
换液频率 |
每周2~3次 |
生物安全等级 |
1 |
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|
种属 |
人源 |
组织来源 |
肺 |
肿瘤类型 |
肺腺癌 |
供体信息 |
61岁,女性,白种人 |
背景描述 |
该细胞1976年分离得到。 |
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|
蛋白表达 |
p53 negative |
同工酶 |
Me-2, 1, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0031 |
致瘤性 |
裸鼠成瘤,形成低分化癌 |
突变信息 |
CaLu-6 cells carry a mutation in KRAS codon 61, c.181C>A p.(Gln61Lys). NRAS of BRAF mutation was not detected. |
核型 |
The stemline chromosome number is hypotriploid and the 2S component occurred at 5.8%. Modal chromosome number is 59. Fourteen marker chromosomes (constitutive) were common to most S metaphases. No Y chromosome was detected in the QM stained preparation. |