• 规格：20μl质粒
• 质粒图谱1： png/1-1F92GJQ3431.png

质粒序列： 点击查看序列

LOCUS       Exported                6038 bp ds-DNA     circular SYN 27-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 6038)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 27, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..6038

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    1..191

                     /label=wt p53 binding sites

     CDS             491..2143

                     /codon_start=1

                     /gene="luc"

                     /product="firefly luciferase"

                     /label=luciferase

                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA

                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP

                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS

                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA

                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK

                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY

                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS

                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL

                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV

                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"

     CDS             2469..2489

                     /codon_start=1

                     /product="nuclear localization signal of SV40 (simian virus

                     40) large T antigen"

                     /label=SV40 NLS

                     /translation="PKKKRKV"

     polyA_signal    2914..3048

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(3539..4127)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4298..5158)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5159..5263)

                     /gene="bla"

                     /label=AmpR promoter

     rep_origin      complement(5289..5744)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        5909..5927

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

ORIGIN

        1 ccaggcaagt ccaggcaggc caggcaagtc caggcaggcc aggcaagtcc aggcaggcca

       61 ggcaagtcca ggcaggccag gcaagtccag gcaggccagg caagtccagg caggccaggc

      121 aagtccaggc agggccaggc aagtccaggc aggccaggca agtccaggca ggccaggcaa

      181 gtccaggcag gatcgaattc gatctgtttt ttttagtatt aagcagaggc cgggggcccc

      241 tggcctccgc ttactctgga gaaaaagaag agaggcattc cagaggcaac ttgtcaaaac

      301 aggactggcg ccttggaggc gctgtggggc cacccaaatt gatataatta agccccaacc

      361 gcctcttccc gcctcatttc agcctcacca ccctcgacaa gcttatcgat accgtcgggg

      421 gatccactag ttctagagcg gccgccaccg cggtggagct cgagcttggc attccggtac

      481 tgttggtaaa atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct

      541 agaggatgga accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc

      601 tggaacaatt gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt

      661 cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag

      721 aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt

      781 tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag

      841 tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt

      901 gaacgtgcaa aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga

      961 ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa

     1021 tgaatacgat tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa

     1081 ttcctctgga tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt

     1141 cagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat

     1201 tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat

     1261 atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct

     1321 tcaggattac aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa

     1381 aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc

     1441 acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg

     1501 acaaggatat gggctcactg agactacatc agctattctg attacacccg agggggatga

     1561 taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga

     1621 taccgggaaa acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat

     1681 tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg

     1741 gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg

     1801 cttgaagtct ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat

     1861 attgttacaa caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc

     1921 cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga

     1981 gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt

     2041 gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga

     2101 gatcctcata aaggccaaga agggcggaaa gtccaaattg taaaatgtaa ctgtattcag

     2161 cgatgacgaa attcttagct attgtaatac tctagaggat ctttgtgaag gaaccttact

     2221 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata

     2281 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga

     2341 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc

     2401 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt

     2461 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc

     2521 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca

     2581 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct

     2641 ttataagtag gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc

     2701 atagagtgtc tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt

     2761 gtaaaggggt taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc

     2821 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac

     2881 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt

     2941 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct

     3001 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcca ctagttctag

     3061 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaatttcga

     3121 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc

     3181 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct

     3241 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc

     3301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt

     3361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag

     3421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca

     3481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt

     3541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc

     3601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct

     3661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg

     3721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca

     3781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact

     3841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta

     3901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta

     3961 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct

     4021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt

     4081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga

     4141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca

     4201 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat

     4261 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg

     4321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt

     4381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag

     4441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc

     4501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag

     4561 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca

     4621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa

     4681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga

     4741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata

     4801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca

     4861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg

     4921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg

     4981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg

     5041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag

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