• 商品货号：FH1576

• 规格：10μl质粒
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LOCUS       Exported                5331 bp ds-DNA     circular SYN 27-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 5331)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 27, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..5331

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        61..364

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        365..568

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             613..1329

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     misc_feature    1369..1989

                     /label=Rab8a-QL

     polyA_signal    2119..2240

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(2247..2702)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2729..2833

                     /gene="bla"

                     /label=AmpR promoter

     promoter        2835..3192

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      3043..3178

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             3227..4021

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    4253..4300

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      4629..5217

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg

       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt

      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca

      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc

      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta

      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac

      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg

      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg

      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt

      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta

      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg

      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc

      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg

      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc

      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag

      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag

      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac

     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac

     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc

     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg

     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc

     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag

     1321 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattcgc gaagacctac

     1381 gattacctgt tcaagctgct gctgatcggg gactcggggg tggggaagac ctgtgtcctg

     1441 ttccgcttct ccgaggacgc cttcaactcc acttttatct ccaccatagg aattgacttt

     1501 aaaattagga ccatagagct cgatggcaag agaattaaac tgcagatatg ggacacagcc

     1561 ggtctggaac ggtttcggac gatcacaacg gcctactaca ggggtgcaat gggcatcatg

     1621 ctggtctacg acatcaccaa cgagaagtcc ttcgacaaca tccggaactg gattcgcaac

     1681 attgaggagc acgcctctgc agacgtcgaa aagatgatac tcgggaacaa gtgtgatgtg

     1741 aatgacaaga gacaagtttc caaggaacgg ggagaaaagc tggccctcga ctatggaatc

     1801 aagttcatgg agaccagcgc gaaggccaac atcaatgtgg aaaatgcatt tttcactctc

     1861 gccagagata tcaaagcaaa aatggacaaa aaattggaag gcaacagccc ccaggggagc

     1921 aaccagggag tcaaaatcac accggaccag cagaagagga gcagcttttt ccgatgtgtt

     1981 cttctgtagg gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta

     2041 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg

     2101 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat

     2161 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc

     2221 aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc

     2281 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc

     2341 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag

     2401 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga

     2461 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc

     2521 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa

     2581 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt

     2641 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc

     2701 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat

     2761 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg

     2821 aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt

     2881 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca

     2941 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat

     3001 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg

     3061 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc

     3121 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta

     3181 ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga

     3241 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc

     3301 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc

     3361 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc

     3421 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac

     3481 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc

     3541 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac

     3601 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg

     3661 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct

     3721 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt

     3781 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg

     3841 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac

     3901 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg

     3961 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg

     4021 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat

     4081 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc

     4141 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg

     4201 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata

     4261 aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc

     4321 cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt

     4381 tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac

     4441 gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta gattgattta

     4501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc

     4561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa

     4621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca

     4681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta

     4741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc

     4801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca

     4861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta

     4921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag

     4981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt