• 规格：20μl质粒
• 质粒简介：           Fluorescent probe for M/G1 transition.
• 质粒图谱1： png/1-1F929155U1F1.png

质粒序列： 点击查看序列

LOCUS       Exported                7475 bp ds-DNA     circular SYN 29-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7475)

  AUTHORS   .

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..7475

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        63..366

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        368..566

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     LTR             584..764

                     /label=5' LTR (truncated)

                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"

     misc_feature    811..936

                     /label=HIV-1 Psi

                     /note="packaging signal of human immunodeficiency virus 

                     type 1"

     misc_feature    1435..1668

                     /label=RRE

                     /note="The Rev response element (RRE) of HIV-1 allows for 

                     Rev-dependent mRNA export from the nucleus to the 

                     cytoplasm."

     misc_feature    2195..2312

                     /label=cPPT/CTS

                     /note="central polypurine tract and central termination 

                     sequence of HIV-1"

     protein_bind    2759..2792

                     /label=loxP

                     /bound_moiety="Cre recombinase"

                     /note="Cre-mediated recombination occurs in the 8-bp core 

                     sequence (GCATACAT)."

     enhancer        2908..3211

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        3212..3415

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             3447..4163

                     /codon_start=1

                     /product="bright green-yellow fluorescent protein derived 

                     from GFP (Lam et al., 2012)"

                     /label=Clover

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL

                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD

                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK

                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL

                     EFVTAAGITHGMDELYK"

     misc_feature    4164..4526

                     /label=Geminin(1-110)

     protein_bind    4562..4595

                     /label=loxP

                     /bound_moiety="Cre recombinase"

                     /note="Cre-mediated recombination occurs in the 8-bp core 

                     sequence (GCATACAT)."

     misc_feature    4651..5239

                     /label=WPRE

                     /note="woodchuck hepatitis virus posttranscriptional 

                     regulatory element"

     LTR             5481..5661

                     /label=5' LTR (truncated)

                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"

     rep_origin      complement(5723..6311)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6482..7342)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7343..7447)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc

       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca

      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt

      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg

      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag

      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt

      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg

      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa

      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt

      541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga

      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata

      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta

      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg

      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa

      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc

      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga

      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat

     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca

     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa

     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag

     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc

     1261 accgcacagc aagcggccgg ccgcgctgat cttcagacct ggaggaggag atatgaggga

     1321 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc

     1381 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc

     1441 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct

     1501 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag

     1561 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca

     1621 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg

     1681 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa

     1741 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa

     1801 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga

     1861 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa

     1921 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat

     1981 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt

     2041 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg

     2101 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc

     2161 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg

     2221 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat

     2281 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc

     2341 cagtttggtt agtaccgggc ccgctctaga gatccgacgc cgccatctct aggcccgcgc

     2401 cggccccctc gcacagactt gtgggagaag ctcggctact cccctgcccc ggttaatttg

     2461 catataatat ttcctagtaa ctatagaggc ttaatgtgcg ataaaagaca gataatctgt

     2521 tctttttaat actagctaca ttttacatga taggcttgga tttctataag agatacaaat

     2581 actaaattat tattttaaaa aacagcacaa aaggaaactc accctaactg taaagtaatt

     2641 gtgtgttttg agactataaa tatcccttgg agaaaagcct tgttaacgcg cggtgaccct

     2701 cgaggtcgac ggtatcgata agctcgcttc acgagattcc agcaggtcga gggacctaat

     2761 aacttcgtat agcatacatt atacgaagtt atattaaggg ttccaagctt aagcggccgc

     2821 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt

     2881 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg

     2941 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac

     3001 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt

     3061 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa

     3121 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta

     3181 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg

     3241 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg

     3301 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc

     3361 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt

     3421 agtgaaccgt cagatccgct agcaccatgg tgagcaaggg cgaggagctg ttcaccgggg

     3481 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtccgcg

     3541 gcgagggcga gggcgatgcc accaacggca agctgaccct gaagttcatc tgcaccaccg

     3601 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc gtggcctgct