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- 规格：20μl质粒
- 启动子：U6 promoter
- 复制子：pUC ori
- 终止子：SV40 poly(A) signal
- 质粒大小：5141bp
- 原核抗性：氨苄青霉素Amp
- 筛选标记：绿色荧光ZsGreen1
- 克隆菌株：大肠杆菌Stbl3
- 质粒简介： pDC316-ZsGreen1-shRNA质粒是一个Admax腺病毒RNA干扰载体，可通过Pst1和BamHI酶切位点连入shRNA片段，并由U6启动子驱动。
- 质粒图谱1： png/1-1G026152011I2.png
质粒序列： 点击查看序列LOCUS Exported 5141 bp ds-DNA circular SYN 26-OCT-2017DEFINITION synthetic circular DNAKEYWORDS pDC316-ZsGreen1-shRNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 5141)AUTHORS .TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..5141/organism="synthetic DNA construct"/mol_type="other DNA"misc_feature 469..992/label=MCMV promoterCDS 1003..1698/codon_start=1/product="Zoanthus green fluorescent protein"/label=ZsGreen1/note="mammalian codon-optimized"/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAINLCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAVCICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKGDVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEHAIASGSALP"polyA_signal 1836..1957/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"promoter 1975..2215/label=U6 promoter/note="RNA polymerase III promoter for human U6 snRNA"polyA_signal 2264..2398/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"protein_bind 2418..2451/label=loxP/bound_moiety="Cre recombinase"/note="Cre-mediated recombination occurs in the 8-bp coresequence (GCATACAT)."rep_origin complement(2875..3463)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(3634..4494)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(4495..4599)/gene="bla"/label=AmpR promotermisc_feature 4682..453/label=AdORIGIN1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gatatactga gtcattaggg481 actttccaat gggttttgcc cagtacataa ggtcaatagg ggtgaatcaa caggaaagtc541 ccattggagc caagtacact gagtcaatag ggactttcca ttgggttttg cccagtacaa601 aaggtcaata gggggtgagt caatgggttt ttcccattat tggcacgtac ataaggtcaa661 taggggtgag tcattgggtt tttccagcca tttaattaaa acgccatgta ctttcccacc721 attgacgtca atgggctatt gaaactaatg caacgtgacc tttaaacggt actttcccat781 agctgattaa tgggaaagta ccgttctcga gccaatacac gtcaatggga agtgaaaggg841 cagccaaaac gtaacaccgc cccggttttc ccctggaaat tccatattgg cactcattct901 attggctgag ctgcgttcta cgtgggtata agaggcgcga ccagcgtcgg taccgtcgca961 gtcttcggtc tgaccaccgt agaacgcaga tcgaattcca ccatggccca gtccaagcac1021 ggcctgacca aggagatgac catgaagtac cgcatggagg gctgcgtgga cggccacaag1081 ttcgtgatca ccggcgaggg catcggctac cccttcaagg gcaagcaggc catcaacctg1141 tgcgtggtgg agggcggccc cttgcccttc gccgaggaca tcttgtccgc cgccttcatg1201 tacggcaacc gcgtgttcac cgagtacccc caggacatcg tcgactactt caagaactcc1261 tgccccgccg gctacacctg ggaccgctcc ttcctgttcg aggacggcgc cgtgtgcatc1321 tgcaacgccg acatcaccgt gagcgtggag gagaactgca tgtaccacga gtccaagttc1381 tacggcgtga acttccccgc cgacggcccc gtgatgaaga agatgaccga caactgggag1441 ccctcctgcg agaagatcat ccccgtgccc aagcagggca tcttgaaggg cgacgtgagc1501 atgtacctgc tgctgaagga cggtggccgc ttgcgctgcc agttcgacac cgtgtacaag1561 gccaagtccg tgccccgcaa gatgcccgac tggcacttca tccagcacaa gctgacccgc1621 gaggaccgca gcgacgccaa gaaccagaag tggcacctga ccgagcacgc catcgcctcc1681 ggctccgcct tgccctaaag atctcgagat ccaccggatc tagataactg atcataatca1741 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga1801 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg1861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt1921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtctgggca ggaagagggc1981 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt2041 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa2101 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt2161 accgtaactt gaaagtattt cgatttcttg ggtttatata tcttgtggaa aggacgaaac2221 acctgcagtt ttttggatcc caatgtcgac ctcgacttcg agcaacttgt ttattgcagc2281 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc2341 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcgt2401 ctagcatcga agatccaata acttcgtata gcatacatta tacgaagtta taagtagctt2461 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca2521 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact2581 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct2641 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc2701 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca2761 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg2821 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca2881 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa2941 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc3001 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc3061 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct3121 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg3181 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag3241 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta3301 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg3361 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt3421 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt3481 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag3541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat3601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc3661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat3721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc3781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag3841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag3901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt3961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg4021 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt4081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc4141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc4201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa4261 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg4321 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc4381 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag4441 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt4501 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt4561 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc4621 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac4681 tctaggcaaa atagcaccct cccgctccag aacaacatac agcgcttcac agcggcagcc4741 taacagtcag ccttaccagt aaaaaagaaa acctattaaa aaaacaccac tcgacacggc4801 accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta tatataggac4861 taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc acgcgaacct4921 acgcccagaa acgaaagcca aaaaacccac aacttcctca aatcgtcact tccgttttcc4981 cacgttacgt aacttcccat tttaagaaaa ctacaattcc caacacatac aagttactcc5041 gccctaaaac ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac5101 cccctcatta tcatattggc ttcaatccaa aataaggtat a//
