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- 规格：20μl质粒
- 复制子：pUC ori
- 质粒大小：7750bp
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：大肠杆菌DH5α
- 备注：高拷贝
- 质粒简介： pPSU2质粒是一个用于制备DNA Marker的高拷贝质粒，如果用Pst1单酶切，会出现50bp,100bp,200bp,300bp,400bp,500bp,600bp,1500bp,4000bp等条带，如果用EcoRI单酶切，会出现750bp,3000bp,4000bp等条带。
- 质粒图谱1： png/1-1G026144002635.png
质粒序列： 点击查看序列LOCUS Exported 7750 bp ds-DNA circular SYN 26-OCT-2017DEFINITION synthetic circular DNASOURCE synthetic DNA constructORGANISM synthetic DNA constructREFERENCE 1 (bases 1 to 7750)AUTHORS .TITLE Direct Submissionhttp://www.fenghbio.cnFEATURES Location/Qualifierssource 1..7750/organism="synthetic DNA construct"/mol_type="other DNA"promoter 5844..5948/gene="bla"/label=AmpR promoterCDS 5949..6809/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"rep_origin 6980..7568/direction=RIGHT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"ORIGIN1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttga241 tatcatcaaa gccatgaaca aagcagccgc gctggatgaa ctgataccgg ggttgctgag301 tgaatatatc gaacagtcag gttaacctgc agcggcattt tgtccgcgcc gggcttcgct361 cactgttcag gccggagcca cagaccgccg ttgaatgggc ggatgctaat tactatctcc421 cgaaagaatc cgcataccag gaagggcgct gggaaacact gccctttcag cgggccatca481 tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt ggtgaagtct gcccgtgtcg541 gttattccaa aatgctgctg ggtgtttatg cctactttat agagcataag cagcgcaaca601 cccttatctg gttgccaacg gatggtgatg ccgagaactt tatgaaaacc cacgttgagc661 cgactattcg tgatattccg tcgctgctgg cgctggcccc gtggtatggc aaaaagcacc721 gggataacac gctcaccatg aagcgtttca ctaatgggcg tggcttctgg tgcctgggcg781 gtaaagcggc aaaaaactac cgtgaaaagt cggtggatgt ggcgggttat gatgaacttg841 ctgcttttga tgatgatatt gaacaggaag gctctccgac gttcctgggt gacaagcgta901 ttgaaggctc ggtctggcca aagtccatcc gtggctccac gccaaaagtg agaggcacct961 gtcagattga gcgtgcagcc agtgaagcga tatcggatct ttccgagcat ttattaagca1021 tttcgctata agttctcgct ggaagaggta gttttttcat tgtactttac cttcatctct1081 gttcattatc atcgctttta aaacggttcg accttctaat cctatctgac cattataatt1141 ttttagaatg gtttcataag aaagctctga atcaacggac tgcgataata agtggtggta1201 tccagaattt gtcacttcaa gtaaaaacac ctcacgagtt aaaacaccta agttctcacc1261 gaatgtctca atatccggac ggataatatt tattgcttct cttgaccgta ggactttcca1321 catgcaggat tttggaacct cttgcagtac tactggggaa tgagttgcaa ttattgctac1381 accattgcgt gcatcgagta agtcgcttaa tgttcgtaaa aaagcagaga gcaaaggtgg1441 atgcagatga acctctggtt catcgaataa aactaatgac ttttcgccaa cgacatctac1501 taatcttgtg atagtaaata aaacaattgc atgtccagag ctcattcgaa gcagatattt1561 ctggatattg tcataaaaca atttagtgaa tttatcatcg tccacttgaa tctgtggttc1621 attacgtctt aactcttcat atttagaaat gaggctgatg agttccatat ttgaaaagtt1681 ttcatcacta cttagttttt tgatagcttc aagccagagt tgtctttttc tatctactct1741 catacaacca ataaatgctg aaatgaattc taagcggaga tcgcctagtg attttaaact1801 attgctggca gcattcttga gtccaactgc agagtattgt gtaccttttg ctgggtcagg1861 ttgttcttta gccggcctgc aggaagagga tggtgcagca accaacaaga aaacactggc1921 agattacgcc cgtgccttat ccggagagga tgaatgacgc gacaggaaga acttgcctgc1981 agtaactttc cccacaacgg aacaactctc attgcatggg atcattgggt actgtgggtt2041 tagtggttgt aaaaacacct gaccgctatc cctgatcagt ttcttgaagg taaactcatc2101 acccccaagt ctggctatgc agaaatcacc tggctcaaca gcctgctcag ggtcaacgag2161 aattaacatt ccgtcactgc agacggctca ggatacggat aacggctact ccgtgtttga2221 gcagtcactg ctgcggtata tcgctgccgg gctgggtgtc tcgtatgagc agctttcccg2281 gaattacgcc cagatgagct actccacggc acgggccagt gcgaacgagt cgtgggcgta2341 ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg agccagatgt ttctgtgctg2401 gctggaagag gccatcgttc gccgcgtggt gacgttacct tcaaaagcgc gcttcagttt2461 tcaggaagcc agatctctgc aggttgacgg ttttccacca tcgcacgccg ggaccatcac2521 cgtgtatgaa gattcacaac cggggacgct gaatgatttt ctctgtgcca tgacggagga2581 tgatgcccgg ccggaggtgc tgcgtcgtct tgaactgatg gtggaagagg tggcgcgtaa2641 cgcgtccgtg gtggcacaga gtacggcaga cgcgaagaaa tcagccggcg atgccagtgc2701 atcagctgct caggtcgcgg cccttgtgac tgatgcaact gactcagcac gcgccgccag2761 cacgtccgcc ggacaggctg catcgtcagc tcaggaagcg tcctccggcg cagaagcggc2821 atcagcaaag gccactgaag cggaaaaaag tgccgcagcc gcagagtcct caaaaactgc2881 aggcggatta caacacgctg atggcggcgg cgaaaaagga ttatgaagcg acgctgaaaa2941 agccgaaaca gtccagcgtg aaggtgtctg cgggcgatcg tcaggaagac agtgctcatg3001 ctgccctgct gacgcttcag gcagaactcc ggacgctgga gaagcatgtc ggagcaaatg3061 agaaaatcag ccagcagcgc cgggatttgt ggaaggcgga gagtcagttc gcggtactgg3121 aggaggcggc gcaacgtcgc cagctgtctg cacaggagaa atccctgctg gcgcataaag3181 atgagacgct ggagtacaaa cgccagctgg ctgcacttgg cgacaaggtt acgtatcagg3241 agcgcctgaa cgcgctggcg cagcaggcgg ataaattcgc acagcagcaa cgggcaaaac3301 gggccgccat tgatgcgaaa agccgggggc tgactgaccg gcaggcagaa cgggaagcca3361 cggaacagcg cctgaactgc agggatccct tctggggccg gacggcatga catcgctgcg3421 cgaatatgcc ggttatcacg gcggtggcag cggatttgga gggcagttgc ggtcgtggaa3481 cccaccgagt gaaagtgtgg atgcagccct gttgcccaac tttacccgtg gcaatgcccg3541 cgcagacgat ctggtacgca ataacggcta tgccgccaac gccatccagc tgcatcagga3601 tcatatcgtc gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat3661 cggggaggaa gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc3721 cgaggatgac tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg3781 ggaaggtgtg gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac3841 cagttcgtcg cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa3901 cccgaacaat accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg3961 tgcggcgctg ggacatctgc agtgtacaga tatccaaatg aagccatagg catttgttat4021 tttggctctg tcagctgcat aacgccaaaa aatatattta tctgcttgat cttcaaatgt4081 tgtattgatt aaatcaattg gatggaattg tttatcataa aaaattaatg tttgaatgtg4141 ataaccgtcc tttaaaaaag tcgtttctgc aagcttggct gtatagtcaa ctaactcttc4201 tgtcgaagtg atatttttag gcttatctac cagttttaga cgctctttaa tatcttcagg4261 aattatttta ttgtcatatt gtatcatgct aaatgacaat ttgcttatgg agtaatcttt4321 taattttaaa taagttattc tcctggcttc atcaaataaa gagtcgaatg atgttggcga4381 aatcacatcg tcacccattg gattgtttat ttgtatgcca agagagttac agcagttata4441 cattctgcca tagattatag ctaaggcatg taataattcg taatctttta gcgtattagc4501 gacccatcgt ctttctgatt taataataga tgattcagtt aaatatgaag gtaatttctt4561 ttgtgcaagt ctgactaact tttttatacc aatgtttaac atactttcat ttgtaataaa4621 ctcaatgtca ttttcttcaa tgtaagatga aataagagta gcctttgcct cgctatacat4681 ttctaaatcg ccttgttttt ctatcgtatt gcgagaattt ttagcccaag ccattaatgg4741 atcatttttc catttttcaa taacattatt gttataccaa atgtcatatc ctataatctg4801 gtttttgttt ttttgaataa taaatgttac tgttcttgcg gtttggagga attgattcaa4861 attcaagcga aataattcag ggtcaaaata tgtatcaatg cagcatttga gcaagtgcga4921 taaatcttta agtcttcttt cccatggttt tttagtcata aaactctcca ttttgatagg4981 ttgcatgcta gatgctgata tattttagag gtgataaaat taactgctta actgtcaatg5041 taatacaagt tgtttgatct ttgcaatgat tcttatcaga aaccatatag taaattagtt5101 acacaggaaa tttttaatat tattattatc attcattatg tattaaaatt agagttgtgg5161 cttggctctg ctaacacgtt gctcatagga gatatggtag agccgcagac acgtcgtatg5221 caggaacgtg ctgtggctgg ctggtgaact tccgatagtg cgggtgttga atgatttcca5281 gttgctaccg attttacata ttttttgcat gagagaattt gtaccacctc ccaccgacca5341 tctatgagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta5401 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg5461 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc5521 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta5581 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg5641 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg5701 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc5761 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag5821 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt5881 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa5941 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt6001 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt6061 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt6121 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg6181 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga6241 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa6301 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga6361 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa6421 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca6481 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta6541 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac6601 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc6661 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag6721 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga6781 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt6841 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata6901 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag6961 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa7021 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt7081 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc7141 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa7201 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa7261 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc7321 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa7381 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa7441 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
