• 规格：20μl质粒
• 启动子：CHOP promoter
• 终止子：SV40 poly(A) signal
• 质粒大小：4880bp
• 原核抗性：卡那霉素Kan
• 筛选标记：新霉素Neo/G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：                CHOP promoter (-649+136) pmCherry-1质粒的CHOP启动子驱动红色荧光蛋白mCherry的表达。
• 质粒图谱1： png/1-1G02416264K32.png

质粒序列： 点击查看序列

LOCUS       Exported                4880 bp ds-DNA     circular SYN 24-OCT-2017

DEFINITION  synthetic circular DNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4880)

  AUTHORS   .

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4880

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    37..817

                     /label=CHOP promoter (-649/+136)

     regulatory      831..840

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             837..1547

                     /codon_start=1

                     /product="monomeric derivative of DsRed fluorescent protein

                     (Shaner et al., 2004)"

                     /label=mCherry

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG

                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF

                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK

                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA

                     EGRHSTGGMDELYK"

     polyA_signal    1668..1789

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(1796..2251)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2278..2382

                     /gene="bla"

                     /label=AmpR promoter

     promoter        2384..2741

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      2592..2727

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             2776..3570

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    3802..3849

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      4178..4766

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tagttattac tagcgctacc ggactcagat ctcgagatgt cgacaatccc agtggatgga

       61 taccaacttt aaaaagaaaa gttcaaaagg cctatgtgcc cattagctgg gaggggccaa

      121 gaaatatggg agtcccttat agtgggggta aaacggcggg taaagctagg tgggcggaac

      181 agcagcttct gggggagaca agcggcaaag aggctcacga ccgactaggg gcgaccaagg

      241 ctgatagccg ttggggccgt tgggcgccgg gagctggcgc cccgccctct ctcctctccc

      301 ccaccctccg cacctcccac caccctcggt gtcccctgcg cgtgcgcgtg cagacaccgg

      361 ttgccaaaca ttgcatcatc cccgcccccc tttcctcccc tccccccccg ctacactccc

      421 ctccgcgcgc gcgcatgact cacccacctc ctccgtgaag cctcgtgacc caaagccact

      481 tccgggtccg acactacgtc gaccccctag cgagagggag cgacgggggc ggtgccgcgg

      541 ggctcctgag tggcggatgc gagggacggg gcgggaccaa tgccggcgtg ccactttctg

      601 attggtaggt tttggggtcc cgcccctgag aggagggcaa ggccatggta aaagattaca

      661 gccaggcgct cccgaggtca gagacttaag tctaaggcac tgagcgtatc atgttaaaga

      721 tgagcgggtg gcagcgacag agccaaaatc agagctggaa cctgaggaga gagtgttcaa

      781 gaaggaagtg tatcttcata catcaccaca cctgacggga tccaccggtc gccaccatgg

      841 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc

      901 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc

      961 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg

     1021 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg

     1081 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga

     1141 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg

     1201 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc

     1261 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc

     1321 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg

     1381 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca

     1441 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg

     1501 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtagcgg ccgcgactct

     1561 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac

     1621 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg

     1681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt

     1741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt

     1801 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt

     1861 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg

     1921 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc

     1981 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca

     2041 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga

     2101 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa

     2161 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc

     2221 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc

     2281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa

     2341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc

     2401 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa

     2461 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc

     2521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc

     2581 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct

     2641 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga

     2701 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga

     2761 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg

     2821 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc

     2881 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt

     2941 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt

     3001 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc

     3061 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc

     3121 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac

     3181 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag

     3241 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag

     3301 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat

     3361 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg

     3421 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa

     3481 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc

     3541 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc

     3601 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt

     3661 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca

     3721 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac

     3781 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt

     3841 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga

     3901 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt

     3961 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag

     4021 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg

     4081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact

     4141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg

     4201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc

     4261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata

     4321 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta

     4381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc

     4441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg

     4501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac

     4561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg

     4621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt

     4681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct

     4741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg

     4801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata

     4861 accgtattac cgccatgcat

//