• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：                pAAV2-8是一个腺相关病毒血清型质粒。
• 质粒图谱1： png/1-1G20510335C25.png

质粒序列： 点击查看序列

LOCUS       Exported               21837 bp ds-DNA     circular SYN 04-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..21837

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     repeat_region   21..123

                     /label=ITR

                     /note="inverted terminal repeat of human adenovirus 

                     serotype 5"

     misc_signal     211..361

                     /label=Ad5 Psi

                     /note="packaging signal for adenovirus serotype 5"

     CDS             1346..3211

                     /codon_start=1

                     /label=Rep

                     /translation="MPGFYEIVIKVPSDLDGHLPGISDSFVNWVAEKEWELPPDSDMDL

                     NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV

                     LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP

                     ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA

                     RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP

                     DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK

                     TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV

                     RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF

                     GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST

                     SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERMNQNSNICFTHGQKDCLECFPVSE

                     SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCIFEQ"

     CDS             3228..5135

                     /codon_start=1

                     /label=Cap

                     /translation="MAADGYLPDWLEDNLSEGIREWWALKPGAPKPKANQQKQDDGRGL

                     VLPGYKYLGPFNGLDKGEPVNAADAAALEHDKAYDQQLQAGDNPYLRYNHADAEFQERL

                     QEDTSFGGNLGRAVFQAKKRVLEPLGLVEEGAKTAPGKKRPVEPSPQRSPDSSTGIGKK

                     GQQPARKRLNFGQTGDSESVPDPQPLGEPPAAPSGVGPNTMAAGGGAPMADNNEGADGV

                     GSSSGNWHCDSTWLGDRVITTSTRTWALPTYNNHLYKQISNGTSGGATNDNTYFGYSTP

                     WGYFDFNRFHCHFSPRDWQRLINNNWGFRPKRLSFKLFNIQVKEVTQNEGTKTIANNLT

                     STIQVFTDSEYQLPYVLGSAHQGCLPPFPADVFMIPQYGYLTLNNGSQAVGRSSFYCLE

                     YFPSQMLRTGNNFQFTYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLSRTQTTGGTA

                     NTQTLGFSQGGPNTMANQAKNWLPGPCYRQQRVSTTTGQNNNSNFAWTAGTKYHLNGRN

                     SLANPGIAMATHKDDEERFFPSNGILILANKMLPETMRITAMSCSPA?KKSKPLTLWLQ

                     RNTVSWQITCSSKTRLLKLELSTARGPYPVWSGRTGTCTCRVPSGPRFLTRTATSTRLR

                     "

     repeat_region   19739..19841

                     /label=ITR

                     /note="inverted terminal repeat of human adenovirus 

                     serotype 5"

     rep_origin      complement(20032..20620)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(20772..21632)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPATMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(21633..21737)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 ggatccgaat tcttaattaa catcatcaat aatatacctt attttggatt gaagccaata

       61 tgataatgag ggggtggagt ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg

      121 tagtagtgtg gcggaagtgt gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg

      181 tggcaaaagt gacgtttttg gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg

      241 gttttaggcg gatgttgtag taaatttggg cgtaaccgag taagatttgg ccattttcgc

      301 gggaaaactg aataagagga agtgaaatct gaataatttt gtgttactca tagcgcgtaa

      361 tatttgtcta gggccgcggg gactttgacc gtttacgtgg agactcgccc aggtgttttt

      421 ctcaggtgtt ttccgcgttc cgggtcaaag ttggcgtttt attattatag tcagggggat

      481 cctctagaac tagtggatcc gtccctcagg cctagaagta aaaaagggaa aaaagagtgt

      541 gtttgtcaaa ataggagaca ggtggtggca accaaggact tataggggac cttacatcta

      601 cagaccaaca gatgccccct taccatatac aggaagatat gacttaaatt gggataggtg

      661 ggtcacaatc aacggctata aagtgttata cagatccctc ccctcccctt tcgtgaaaga

      721 ctcgccagag ctagacctcc ttggtgtatg ctaactgaga agagaaagac gacatgaaac

      781 aacaggtaca tgattatatt tatctaggaa caggaatgca cttttgggga aaggttttcc

      841 ataccaagga aggggcagtg gctggactga tagaacatta ttctgcaaaa acttatggta

      901 tgagttatta tgattagcct ttatttgccc aaccttgcgg ttcccagggt ttaaataagt

      961 ttatggttac aaactgttct taaaacgagg atgtgagaca agtggtttcc tgacttggtt

     1021 tggtaatcaa atgttctgat ctgagctctt agtgttctat tttcctatgt tcttttggaa

     1081 tctatccaag tcttatgtaa atgcttatgt aaaccataat ataaaagagt gctgattttt

     1141 tgagtaaact tgcaacagtc ctaacattct tctctcgtgt gtttgtgtct gttcgccatc

     1201 ccgtctccgc tcgtcactta tccttcactt ttcagagggt ccccccgcag atcccggtca

     1261 ccctcaggtc gggacctgca gaagacgccc gagtgagcac gcagggtctc cattttgaag

     1321 cgggaggttt gaacgcgcag ccgccatgcc ggggttttac gagattgtga ttaaggtccc

     1381 cagcgacctt gacgggcatc tgcccggcat ttctgacagc tttgtgaact gggtggccga

     1441 gaaggaatgg gagttgccgc cagattctga catggatctg aatctgattg agcaggcacc

     1501 cctgaccgtg gccgagaagc tgcagcgcga ctttctgacg gaatggcgcc gtgtgagtaa

     1561 ggccccggag gcccttttct ttgtgcaatt tgagaaggga gagagctact tccacatgca

     1621 cgtgctcgtg gaaaccaccg gggtgaaatc catggttttg ggacgtttcc tgagtcagat

     1681 tcgcgaaaaa ctgattcaga gaatttaccg cgggatcgag ccgactttgc caaactggtt

     1741 cgcggtcaca aagaccagaa atggcgccgg aggcgggaac aaggtggtgg atgagtgcta

     1801 catccccaat tacttgctcc ccaaaaccca gcctgagctc cagtgggcgt ggactaatat

     1861 ggaacagtat ttaagcgcct gtttgaatct cacggagcgt aaacggttgg tggcgcagca

     1921 tctgacgcac gtgtcgcaga cgcaggagca gaacaaagag aatcagaatc ccaattctga

     1981 tgcgccggtg atcagatcaa aaacttcagc caggtacatg gagctggtcg ggtggctcgt

     2041 ggacaagggg attacctcgg agaagcagtg gatccaggag gaccaggcct catacatctc

     2101 cttcaatgcg gcctccaact cgcggtccca aatcaaggct gccttggaca atgcgggaaa

     2161 gattatgagc ctgactaaaa ccgcccccga ctacctggtg ggccagcagc ccgtggagga

     2221 catttccagc aatcggattt ataaaatttt ggaactaaac gggtacgatc cccaatatgc

     2281 ggcttccgtc tttctgggat gggccacgaa aaagttcggc aagaggaaca ccatctggct

     2341 gtttgggcct gcaactaccg ggaagaccaa catcgcggag gccatagccc acactgtgcc

     2401 cttctacggg tgcgtaaact ggaccaatga gaactttccc ttcaacgact gtgtcgacaa

     2461 gatggtgatc tggtgggagg aggggaagat gaccgccaag gtcgtggagt cggccaaagc

     2521 cattctcgga ggaagcaagg tgcgcgtgga ccagaaatgc aagtcctcgg cccagataga

     2581 cccgactccc gtgatcgtca cctccaacac caacatgtgc gccgtgattg acgggaactc

     2641 aacgaccttc gaacaccagc agccgttgca agaccggatg ttcaaatttg aactcacccg

     2701 ccgtctggat catgactttg ggaaggtcac caagcaggaa gtcaaagact ttttccggtg

     2761 ggcaaaggat cacgtggttg aggtggagca tgaattctac gtcaaaaagg gtggagccaa

     2821 gaaaagaccc gcccccagtg acgcagatat aagtgagccc aaacgggtgc gcgagtcagt

     2881 tgcgcagcca tcgacgtcag acgcggaagc ttcgatcaac tacgcagaca ggtaccaaaa

     2941 caaatgttct cgtcacgtgg gcatgaatct gatgctgttt ccctgcagac aatgcgagag

     3001 aatgaatcag aattcaaata tctgcttcac tcacggacag aaagactgtt tagagtgctt

     3061 tcccgtgtca gaatctcaac ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta

     3121 cattcatcat atcatgggaa aggtgccaga cgcttgcact gcctgcgatc tggtcaatgt

     3181 ggatttggat gactgcatct ttgaacaata aatgatttaa atcaggtatg gctgccgatg

     3241 gttatcttcc agattggctc gaggacaacc tctctgaggg cattcgcgag tggtgggcgc

     3301 tgaaacctgg agccccgaag cccaaagcca accagcaaaa gcaggacgac ggccggggtc

     3361 tggtgcttcc tggctacaag tacctcggac ccttcaacgg actcgacaag ggggagcccg

     3421 tcaacgcggc ggacgcagcg gccctcgagc acgacaaggc ctacgaccag cagctgcagg

     3481 cgggtgacaa tccgtacctg cggtataacc acgccgacgc cgagtttcag gagcgtctgc

     3541 aagaagatac gtcttttggg ggcaacctcg ggcgagcagt cttccaggcc aagaagcggg

     3601 ttctcgaacc tctcggtctg gttgaggaag gcgctaagac ggctcctgga aagaagagac

     3661 cggtagagcc atcaccccag cgttctccag actcctctac gggcatcggc aagaaaggcc

     3721 aacagcccgc cagaaaaaga ctcaattttg gtcagactgg cgactcagag tcagttccag

     3781 accctcaacc tctcggagaa cctccagcag cgccctctgg tgtgggacct aatacaatgg

     3841 ctgcaggcgg tggcgcacca atggcagaca ataacgaagg cgccgacgga gtgggtagtt

     3901 cctcgggaaa ttggcattgc gattccacat ggctgggcga cagagtcatc accaccagca

     3961 cccgaacctg ggccctgccc acctacaaca accacctcta caagcaaatc tccaacggga

     4021 catcgggagg agccaccaac gacaacacct acttcggcta cagcaccccc tgggggtatt

     4081 ttgactttaa cagattccac tgccactttt caccacgtga ctggcagcga ctcatcaaca

     4141 acaactgggg attccggccc aagagactca gcttcaagct cttcaacatc caggtcaagg

     4201 aggtcacgca gaatgaaggc accaagacca tcgccaataa cctcaccagc accatccagg

     4261 tgtttacgga ctcggagtac cagctgccgt acgttctcgg ctctgcccac cagggctgcc

     4321 tgcctccgtt cccggcggac gtgttcatga ttccccagta cggctaccta acactcaaca

     4381 acggtagtca ggccgtggga cgctcctcct tctactgcct ggaatacttt ccttcgcaga

     4441 tgctgagaac cggcaacaac ttccagttta cttacacctt cgaggacgtg cctttccaca

     4501 gcagctacgc ccacagccag agcttggacc ggctgatgaa tcctctgatt gaccagtacc

     4561 tgtactactt gtctcggact caaacaacag gaggcacggc aaatacgcag actctgggct

     4621 tcagccaagg tgggcctaat acaatggcca atcaggcaaa gaactggctg ccaggaccct

     4681 gttaccgcca acaacgcgtc tcaacgacaa ccgggcaaaa caacaatagc aactttgcct

     4741 ggactgctgg gaccaaatac catctgaatg gaagaaattc attggctaat cctggcatcg

     4801 ctatggcaac acacaaagac gacgaggagc gtttttttcc cagtaacggg atcctgattt

     4861 tggcaaacaa aatgctgcca gagacaatgc ggattacagc gatgtcatgc tcaccagcga

     4921 gnaagaaatc aaaaccacta accctgtggc tacagaggaa tacggtatcg tggcagataa

     4981 cttgcagcag caaaacacgg ctcctcaaat tggaactgtc aacagccagg gggccttacc

     5041 cggtatggtc tggcagaacc gggacgtgta cctgcagggt cccatctggg ccaagattcc