- 商品货号:FH1655
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 筛选标记:红色荧光蛋白mCherry
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: pHR_Gal4UAS_Pembrolizumab_heavychain_T2A_ Pembrolizumab_lightchain_PGK_mCherry是一个哺乳细胞抗体表达质粒。
- 质粒图谱1: png/1-1G204093I0200.png
质粒序列: 点击查看序列LOCUS Exported 12264 bp ds-DNA circular SYN 03-JAN-2016FEATURES Location/Qualifierssource 1..12264/organism="synthetic DNA construct"/mol_type="other DNA"misc_feature 96..7517/label=match to pHR-CMVLacZ/note="match to pHR-CMVLacZ"/note="color: #a6acb3"primer_bind complement(320..337)/label=SP6/note="SP6"/note="color: #00ff00; direction: LEFT"CDS 1231..2091/codon_start=1/label=bla (AmpR)/note="bla"/note="color: #993366"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"CDS 1429..2088/label=AmpR/note="AmpR"/note="color: #ffff00; direction: RIGHT"rep_origin 2186..2868/label=ColE1 origin/note="ColE1 origin"/note="color: #808080"promoter 3084..3410/label=SV40 - Invitrogen/note="SV40 - Invitrogen promoter"/note="color: #0000ff"promoter 3229..3425/label=SV40 early promoter/note="SV40 early promoter"/note="color: #6495ed"polyA_site 5105..5123/label=SV40 pA/note="SV40 pA"/note="color: #ccff66"polyA_signal complement(5138..5329)/label=SV40 late polyA/note="SV40 late polyA"/note="color: #ffc0cb; direction: LEFT"polyA_site 5199..5330/label=SV40 early poly A/note="SV40 early poly A"/note="color: #80ff00"polyA_site 5199..5329/label=SV40 early poly A (2)/note="SV40 early poly A"/note="color: #66ff66"repeat_region 7518..7697/label=cPPT/note="cPPT"/note="color: #a6acb3"primer_bind 7577..7598/note="QB2741"/note="color: #ff0000; direction: RIGHT"primer_bind 7673..7696/note="cPPT_5'seq"/note="color: #a6acb3; direction: RIGHT"misc_feature 7716..7721/note="EcoRI"/note="color: #a6acb3"misc_feature 7722..7807/note="Gal4"/note="color: #a6acb3"misc_feature 7828..7847/note="Gal4"/note="color: #a6acb3"misc_feature 7848..7853/note="SpeI"/note="color: #a6acb3"promoter 7854..7971/note="mCMV"/note="color: #ffffff"misc_feature 7972..7991/note="20BPhomo BamHI-pHR Gal4UAS synNotch_fwd"/note="color: #a6acb3"misc_feature 7992..9445/note="Pembrolizumab_HC_gBlock"/note="color: #a6acb3"misc_feature 7992..7997/note="Kozak Sequence"/note="color: #ff00ff"misc_feature 7998..8054/note="Human IgG Heavy Chain Signal Peptide"/note="Kober et al 2013 Biotech and BioengCodon Optimized"/note="color: #ff0000"misc_feature 8055..8084/note="myc tag"/note="label=myc tag - ApEinfo_fwdcolor=#ffff80 -ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data{{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 165..194"/note="color: #dc143c; direction: RIGHT"misc_feature 8055..8084/note="cw35"/note="label=cw35 - ApEinfo_fwdcolor=#8080c0 -ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data{{0 1 2 0 0 -1} {} 0} - width 5 offset 0 - 165..214"/note="color: #dc143c; direction: RIGHT"misc_feature complement(8055..8084)/note="cw34"/note="label=cw34 - ApEinfo_fwdcolor=cyan -ApEinfo_revcolor=green - ApEinfo_graphicformat=arrow_data{{0 1 2 0 0 -1} {} 0} - width 5 offset 0 -complement(145..194)"/note="color: #00ffff; direction: LEFT"misc_feature 8055..8084/product="Myc (human c-Myc oncogene) epitope tag"/note="Myc"/note="color: #cc99b2"misc_feature 8085..9425/note="Pembrolizumab Heavy Chain"/note="IDT Codon Optimized (gBlocks setting, homo sapiens)From AA :http://www.drugbank.ca/drugs/DB09037/polypeptide_sequences.fasta"/note="color: #00ff00"misc_feature 8085..8444/note="Pembrolizumab HC Variable"/note="color: #0000ff"misc_feature 8445..9425/note="huIgG4 Constant"/note=" (http://www.uniprot.org/uniprot/P01861)"/note="color: #00ffff"misc_feature 8766..8768/note="S -> P"/note="color: #993366"misc_feature 9426..10244/note="Pembrolizumab_LC_gBlock"/note="color: #a6acb3"misc_feature 9426..9560/note="mAb_Center_gBlock"/note="color: #ffcc99"misc_feature 9426..9437/note="Furin Cleavage Site"/note="color: #ff0000"misc_feature 9438..9449/note="SGSG Spacer"/note="color: #a6acb3"misc_feature 9450..9503/note="T2A"/note="Codon optimized from AA sequence from Yang et al.2008"/note="color: #ffcc00"misc
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