• 规格：20μl质粒
• 原核抗性：卡那霉素Kan
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：            pLTR-RD114A慢病毒RD114包膜蛋白表达包装质粒。
• 质粒图谱1： jpg/1-1G2040ZU13C.jpg

质粒序列： 点击查看序列

LOCUS       Exported                6258 bp ds-DNA     circular SYN 03-NOV-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..6258

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     polyA_signal    3046..3167

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(3174..3629)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        3656..3760

                     /gene="bla"

                     /label=AmpR promoter

     promoter        3762..4119

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      3970..4105

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             4154..4948

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=NeoR/KanR

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    5180..5227

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

     rep_origin      5556..6144

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

ORIGIN

        1 tagttattac cggactcaga tctcgagacc tagaaaaaca tggagcaatc acaagtagca

       61 atacagcagc taacaatgct gcttgtgcct ggctagaagc acaagaggag gaagaggtgg

      121 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc

      181 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac

      241 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact

      301 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac

      361 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc

      421 ctgtgagcct gcatggaatg gatgaccctg agagagaagt gttagagtgg aggtttgaca

      481 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct

      541 gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg

      601 gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct ttttgcctgt

      661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac

      721 ccactgctta agcctcaata aagctgatcc cccgggctgc aggaatttat gaaatccttt

      781 atgggggacc cccccctttg tcaaccttgc tcaattcctt ctccccctcc gatcctaaga

      841 ctgatttaca agcccgacta aaagggctgc aaggcgtgca ggcccaaatc tggacacccc

      901 tggccgaatt gtaccggcca ggacatccac aaactagcca cccatttcag gtgggagact

      961 ccgtgtacgt ccggcggcac cgctctcaag gattggagcc tcgttggaag ggaccttaca

     1021 tcgtcctgct gaccacgccc accgccataa aggttgacgg gatcgccgcc tggattcacg

     1081 catcgcacgc caaggcagcc ccaaaaaccc ctggaccaga aactcccaaa acctggaagc

     1141 tccgccgttc ggagaaccct cttaagataa gactctcccg tgtctgactg ctaatccacc

     1201 ttgtccctgt actaacccaa aatgaaactc ccaacaggaa tggtcatttt atgtagccta

     1261 ataatagttc gggcagggtt tgacgacccc cgcaaggcta tcgcattagt acaaaaacaa

     1321 catggtaaac catgcgaatg cagcggaggg caggtatccg aggccccacc gaactccatc

     1381 caacaggtaa cttgcccagg caagacggcc tacttaatga ccaaccaaaa atggaaatgc

     1441 agagtcactc caaaaaatct cacccctagc gggggagaac tccagaactg cccctgtaac

     1501 actttccagg actcgatgca cagttcttgt tatactgaat accggcaatg cagggcgaat

     1561 aataagacat actacacggc caccttgctt aaaatacggt ctgggagcct caacgaggta

     1621 cagatattac aaaaccccaa tcagctccta cagtcccctt gtaggggctc tataaatcag

     1681 cccgtttgct ggagtgccac agcccccatc catatctccg atggtggagg acccctcgat

     1741 actaagagag tgtggacagt ccaaaaaagg ctagaacaaa ttcataaggc tatgcatcct

     1801 gaacttcaat accacccctt agccctgccc aaagtcagag atgaccttag ccttgatgca

     1861 cggacttttg atatcctgaa taccactttt aggttactcc agatgtccaa ttttagcctt

     1921 gcccaagatt gttggctctg tttaaaacta ggtaccccta cccctcttgc gatacccact

     1981 ccctctttaa cctactccct agcagactcc ctagcgaatg cctcctgtca gattatacct

     2041 cccctcttgg ttcaaccgat gcagttctcc aactcgtcct gtttatcttc ccctttcatt

     2101 aacgatacgg aacaaataga cttaggtgca gtcaccttta ctaactgcac ctctgtagcc

     2161 aatgtcagta gtcctttatg tgccctaaac gggtcagtct tcctctgtgg aaataacatg

     2221 gcatacacct atttacccca aaactggaca ggactttgcg tccaagcctc cctcctcccc

     2281 gacattgaca tcatcccggg ggatgagcca gtccccattc ctgccattga tcattatata

     2341 catagaccta aacgagctgt acagttcatc cctttactag ctggactggg aatcaccgca

     2401 gcattcacca ccggagctac aggcctaggt gtctccgtca cccagtatac aaaattatcc

     2461 catcagttaa tatctgatgt ccaagtctta tccggtacca tacaagattt acaagaccag

     2521 gtagactcgt tagctgaagt agttctccaa aataggaggg gactggacct actaacggca

     2581 gaacaaggag gaatttgttt agccttacaa gaaaaatgct gtttttatgc taacaagtca

     2641 ggaattgtga gaaacaaaat aagaacccta caagaagaat tacaaaaacg cagggaaagc

     2701 ctggcatcca accctctctg gaccgggctg cagggctttc ttccgtacct cctacctctc

     2761 ctgggacccc tactcaccct cctactcata ctaaccattg ggccatgcgt tttcaatcga

     2821 ttggtccaat ttgttaaaga caggatctca gtggtccagg ctctggtttt gactcagcaa

     2881 tatcaccagc taaaacccat agagtacgag ccatgaagat ccaccggatc tagataactg

     2941 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac

     3001 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca

     3061 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt

     3121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt

     3181 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa

     3241 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt

     3301 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa

     3361 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag

     3421 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt

     3481 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg

     3541 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc

     3601 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg

     3661 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata

     3721 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag

     3781 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt

     3841 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca

     3901 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta

     3961 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga

     4021 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag

     4081 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg

     4141 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt

     4201 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt

     4261 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc

     4321 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc

     4381 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga

     4441 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat

     4501 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca

     4561 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga

     4621 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc

     4681 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat

     4741 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga

     4801 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg

     4861 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt

     4921 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa

     4981 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg

     5041 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg

     5101 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg

     5161 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg

     5221 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga

     5281 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg

     5341 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt

     5401 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg

     5461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga

     5521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta

     5581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa

     5641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact

     5701 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca

     5761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt

     5821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg

     5881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag

     5941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta

     6001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat

     6061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg

     6121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc

     6181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac

     6241 cgtattaccg ccatgcat

//