• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：URA3
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：               pT040是一个酵母细胞gRNA表达质粒。
• 质粒图谱1： png/1-1G20312444R14.png

质粒序列： 点击查看序列

LOCUS       Exported                5186 bp ds-DNA     circular SYN 25-NOV-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5186

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        27..131

                     /gene="bla"

                     /label=AmpR promoter

     CDS             132..992

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      1163..1751

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     terminator      2378..2397

                     /label=SUP4 terminator

                     /note="transcription terminator for the S. cerevisiae SUP4 

                     tRNA gene"

     promoter        complement(2479..2747)

                     /label=SNR52 promoter

                     /note="promoter for the S. cerevisiae small nucleolar RNA 

                     gene SNR52"

     promoter        4089..4304

                     /gene="S. cerevisiae URA3"

                     /label=URA3 promoter

     CDS             4305..5108

                     /codon_start=1

                     /gene="S. cerevisiae URA3"

                     /product="orotidine-5'-phosphate decarboxylase, required 

                     for uracil biosynthesis"

                     /label=URA3

                     /note="yeast auxotrophic marker, counterselectable with 

                     5-fluoroorotic acid (5-FOA)"

                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL

                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ

                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE

                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD

                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"

ORIGIN

        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac

       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa

      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat

      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc

      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga

      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg

      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc

      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag

      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc

      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg

      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg

      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac

      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac

      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg

      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg

      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg

      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac

     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg

     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg

     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc

     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc

     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt

     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc

     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact

     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac

     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag

     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg

     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg

     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga

     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt

     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct

     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg

     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt

     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta

     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta

     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt

     2161 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtaccg ggccccccct

     2221 cgatcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc ccgctcgagc

     2281 gggggggatc cactagttct agagcggccg tggtatcgtt tagattggca attacagtgt

     2341 cttagctcac atgcttataa ctaattacat gactcgaaga cataaaaaac aaaaaaagca

     2401 ccaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta

     2461 tttaaatgca tcgattctga tcatttatct ttcactgcgg agaagtttcg aacgccgaaa

     2521 catgcgcacc aactttcact tctacagcgt ttgaccaaaa tcttttgaac agaacattgt

     2581 agggtgtgaa aaaatgcgca cctttaccgc tagcccaaga gggcactaca aaatctagag

     2641 ttgtacttca aacgtacatg taatcacctt gtatatactc gaaagaaaac atcaagtttc

     2701 tgtataaata tgagtgaaag cataatcata cattatcttt tcaaagattg tccgcggtgg

     2761 agctccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt

     2821 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc

     2881 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc

     2941 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca

     3001 caccgcataa ctagctttta acttgtatcc taggttatct atgctgtctc accatagaga

     3061 atattaccta tttcagaatg tatgtccatg attcgccggg taaatacata taatacacaa

     3121 atctggctta ataaagtcta taatatatct cataaagaag tgctaaattg gctagtgcta

     3181 tatattttta agaaaatttc ttttgactaa gtccatatcg actttgtaaa agttcacttt

     3241 agcatacata tattacacga gccagaaatt gtaacttttg cctaaaatca caaattgcaa

     3301 aatttaattg cttgcaaaag gtcacatgct tataatcaac ttttttaaaa atttaaaata

     3361 cttttttatt ttttattttt aaacataaat gaaataattt atttattgtt tatgattacc

     3421 gaaacataaa acctgctcaa gaaaaagaaa ctgttttgtc cttggaaaaa aagcactacc

     3481 taggagcggc caaaatgccg aggctttcat agcttaaact ctttacagaa aataggcatt

     3541 atagatcagt tcgagttttc ttattcttcc ttccggtttt atcgtcacag ttttacagta

     3601 aataagtatc acctcttaga gttggcggtt gtttgcaaga ccgagaaaag gctagcaaga

     3661 atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt gattccattg

     3721 cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa tcaatcaaaa

     3781 agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta tgtttagatc

     3841 ttttatgctt gcttttcaaa aggcctgcag gcaagtgcac aaacaatact taaataaata

     3901 ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc

     3961 ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct

     4021 aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat gtaagctaag

     4081 cttttcaatt caattcatca tttttttttt attctttttt ttgatttcgg tttctttgaa

     4141 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta

     4201 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa

     4261 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat

     4321 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc

     4381 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg

     4441 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga

     4501 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt

     4561 ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact

     4621 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg

     4681 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag

     4741 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta

     4801 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa

     4861 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt

     4921 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta

     4981 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg

     5041 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc

     5101 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt

     5161 caatttaatt atatcagtta ttaccc

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