• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：             pBABE-neo-hTERT是一个人源基因逆病毒表达质粒。
• 质粒图谱1： png/1-1G203115F6231.png

质粒序列： 点击查看序列

LOCUS       Exported                8709 bp ds-DNA     circular SYN 03-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..8709

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     LTR             6..475

                     /note="long terminal repeat from Moloney murine leukemia 

                     virus"

     misc_feature    540..739

                     /label=MMLV Psi

                     /note="packaging signal of Moloney murine leukemia virus 

                     (MMLV)"

     CDS             940..1356

                     /label=gag (truncated)

                     /note="truncated Moloney murine leukemia virus (MMLV) gag 

                     gene lacking the start codon"

     CDS             1436..4834

                     /codon_start=1

                     /label=hTert

                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA

                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL

                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA

                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR

                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE

                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ

                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN

                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP

                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC

                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY

                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN

                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV

                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG

                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF

                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL

                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW

                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL

                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK

                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL

                     PGTTLTALEAAANPALPSDFKTILD"

     promoter        4851..5180

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      5031..5166

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             5537..6331

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     LTR             7135..7604

                     /note="long terminal repeat from Moloney murine leukemia 

                     virus"

     CDS             complement(7719..8579)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(8580..8684)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg

       61 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc

      121 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt

      181 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt

      241 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc

      301 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc

      361 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg

      421 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg

      481 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca

      541 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg

      601 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact

      661 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt

      721 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg

      781 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg

      841 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt

      901 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc

      961 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta

     1021 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc

     1081 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc

     1141 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc

     1201 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct

     1261 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc

     1321 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcccagt gtggtggtac

     1381 gtaggaattc gtcctgctgc gcacgtggga agccctggcc ccggccaccc ccgcgatgcc

     1441 gcgcgctccc cgctgccgag ccgtgcgctc cctgctgcgc agccactacc gcgaggtgct

     1501 gccgctggcc acgttcgtgc ggcgcctggg gccccagggc tggcggctgg tgcagcgcgg

     1561 ggacccggcg gctttccgcg cgctggtggc ccagtgcctg gtgtgcgtgc cctgggacgc

     1621 acggccgccc cccgccgccc cctccttccg ccaggtgtcc tgcctgaagg agctggtggc

     1681 ccgagtgctg cagaggctgt gcgagcgcgg cgcgaagaac gtgctggcct tcggcttcgc

     1741 gctgctggac ggggcccgcg ggggcccccc cgaggccttc accaccagcg tgcgcagcta

     1801 cctgcccaac acggtgaccg acgcactgcg ggggagcggg gcgtgggggc tgctgctgcg

     1861 ccgcgtgggc gacgacgtgc tggttcacct gctggcacgc tgcgcgctct ttgtgctggt

     1921 ggctcccagc tgcgcctacc aggtgtgcgg gccgccgctg taccagctcg gcgctgccac

     1981 tcaggcccgg cccccgccac acgctagtgg accccgaagg cgtctgggat gcgaacgggc

     2041 ctggaaccat agcgtcaggg aggccggggt ccccctgggc ctgccagccc cgggtgcgag

     2101 gaggcgcggg ggcagtgcca gccgaagtct gccgttgccc aagaggccca ggcgtggcgc

     2161 tgcccctgag ccggagcgga cgcccgttgg gcaggggtcc tgggcccacc cgggcaggac

     2221 gcgtggaccg agtgaccgtg gtttctgtgt ggtgtcacct gccagacccg ccgaagaagc

     2281 cacctctttg gagggtgcgc tctctggcac gcgccactcc cacccatccg tgggccgcca

     2341 gcaccacgcg ggccccccat ccacatcgcg gccaccacgt ccctgggaca cgccttgtcc

     2401 cccggtgtac gccgagacca agcacttcct ctactcctca ggcgacaagg agcagctgcg

     2461 gccctccttc ctactcagct ctctgaggcc cagcctgact ggcgctcgga ggctcgtgga

     2521 gaccatcttt ctgggttcca ggccctggat gccagggact ccccgcaggt tgccccgcct

     2581 gccccagcgc tactggcaaa tgcggcccct gtttctggag ctgcttggga accacgcgca

     2641 gtgcccctac ggggtgctcc tcaagacgca ctgcccgctg cgagctgcgg tcaccccagc

     2701 agccggtgtc tgtgcccggg agaagcccca gggctctgtg gcggcccccg aggaggagga

     2761 cacagacccc cgtcgcctgg tgcagctgct ccgccagcac agcagcccct ggcaggtgta

     2821 cggcttcgtg cgggcctgcc tgcgccggct ggtgccccca ggcctctggg gctccaggca

     2881 caacgaacgc cgcttcctca ggaacaccaa gaagttcatc tccctgggga agcatgccaa

     2941 gctctcgctg caggagctga cgtggaagat gagcgtgcgg ggctgcgctt ggctgcgcag

     3001 gagcccaggg gttggctgtg ttccggccgc agagcaccgt ctgcgtgagg agatcctggc

     3061 caagttcctg cactggctga tgagtgtgta cgtcgtcgag ctgctcaggt ctttctttta

     3121 tgtcacggag accacgtttc aaaagaacag gctctttttc taccggaaga gtgtctggag

     3181 caagttgcaa agcattggaa tcagacagca cttgaagagg gtgcagctgc gggagctgtc

     3241 ggaagcagag gtcaggcagc atcgggaagc caggcccgcc ctgctgacgt ccagactccg

     3301 cttcatcccc aagcctgacg ggctgcggcc gattgtgaac atggactacg tcgtgggagc

     3361 cagaacgttc cgcagagaaa agagggccga gcgtctcacc tcgagggtga aggcactgtt

     3421 cagcgtgctc aactacgagc gggcgcggcg ccccggcctc ctgggcgcct ctgtgctggg

     3481 cctggacgat atccacaggg cctggcgcac cttcgtgctg cgtgtgcggg cccaggaccc

     3541 gccgcctgag ctgtactttg tcaaggtgga tgtgacgggc gcgtacgaca ccatccccca

     3601 ggacaggctc acggaggtca tcgccagcat catcaaaccc cagaacacgt actgcgtgcg

     3661 tcggtatgcc gtggtccaga aggccgccca tgggcacgtc cgcaaggcct tcaagagcca

     3721 cgtctctacc ttgacagacc tccagccgta catgcgacag ttcgtggctc acctgcagga

     3781 gaccagcccg ctgagggatg ccgtcgtcat cgagcagagc tcctccctga atgaggccag

     3841 cagtggcctc ttcgacgtct tcctacgctt catgtgccac cacgccgtgc gcatcagggg

     3901 caagtcctac gtccagtgcc aggggatccc gcagggctcc atcctctcca cgctgctctg

     3961 cagcctgtgc tacggcgaca tggagaacaa gctgtttgcg gggattcggc gggacgggct

     4021 gctcctgcgt ttggtggatg atttcttgtt ggtgacacct cacctcaccc acgcgaaaac

     4081 cttcctcagg accctggtcc gaggtgtccc tgagtatggc tgcgtggtga acttgcggaa

     4141 gacagtggtg aacttccctg tagaagacga ggccctgggt ggcacggctt ttgttcagat

     4201 gccggcccac ggcctattcc cctggtgcgg cctgctgctg gatacccgga ccctggaggt

     4261 gcagagcgac tactccagct atgcccggac ctccatcaga gccagtctca ccttcaaccg

     4321 cggcttcaag gctgggagga acatgcgtcg caaactcttt ggggtcttgc ggctgaagtg