- 商品货号:FH1670
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 克隆菌株:大肠杆菌Stbl3
- 质粒简介: pJL-SaKKH-BE3是一个大鼠基因哺乳过表达质粒。
- 质粒图谱1: png/1-1G201164ZD32.png
质粒序列: 点击查看序列LOCUS Exported 7689 bp ds-DNA circular SYN 01-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..7689/organism="synthetic DNA construct"/mol_type="other DNA"CDS 13..261/codon_start=1/product="uracil-DNA glycosylase inhibitor from a Bacillussubtilis bacteriophage (Mol et al., 1995)"/label=UGI/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTAYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"CDS 274..294/codon_start=1/product="nuclear localization signal of SV40 (simian virus40) large T antigen"/label=SV40 NLS/translation="PKKKRKV"CDS 303..320/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"polyA_signal 349..573/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin complement(1046..1634)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(1805..2665)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(2666..2770)/gene="bla"/label=AmpR promoterenhancer 3040..3419/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 3420..3623/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 3665..3683/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"CDS 3697..4383/codon_start=1/product="cytidine deaminase (C to U editing enzyme) fromrat"/label=APOBEC-1/note="can use ssDNA as a substrate (Komor et al., 2016)"/translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHILWATGLK"misc_feature 4432..7596/label=SaKKHCas9nCDS 7597..7617/codon_start=1/product="nuclear localization signal of SV40 (simian virus40) large T antigen"/label=SV40 NLS/translation="PKKKRKV"CDS 7624..7689/codon_start=1/product="three tandem FLAG(R) epitope tags, followed by anenterokinase cleavage site"/label=3xFLAG/translation="DYKDHDGDYKDHDIDYKDDDDK"ORIGIN1 tctggtggtt ctactaatct gtcagatatt attgaaaagg agaccggtaa gcaactggtt61 atccaggaat ccatcctcat gctcccagag gaggtggaag aagtcattgg gaacaagccg121 gaaagcgata tactcgtgca caccgcctac gacgagagca ccgacgagaa tgtcatgctt181 ctgactagcg acgcccctga atacaagcct tgggctctgg tcatacagga tagcaacggt241 gagaacaaga ttaagatgct ctctggtggt tctcccaaga agaagaggaa agtctaaccg301 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta361 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca421 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc481 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata541 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg601 gctcgatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg661 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta721 aagcctaggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg781 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga841 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc1021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca1081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt1141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc1201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc1261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc1321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact1381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg1441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta1501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca1561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa1621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg1681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc1741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg1801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat1861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg1921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa1981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca2041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc2101 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt2161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa2221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat2281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct2341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga2401 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag2461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga2521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca2581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg2641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc2701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag2761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat2821 ctcccgatcc cctagggtcg actctcagta caatctgctc tgatgccgca tagttaagcc2881 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag2941 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt3001 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt3061 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta3121 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt3181 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg3241 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta3301 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga3361 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg3421 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc3481 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact3541 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt3601 gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag agatccgcgg3661 ccgctaatac gactcactat agggagagcc gccaccatga gctcagagac tggcccagtg3721 gctgtggacc ccacattgag acggcggatc gagccccatg agtttgaggt attcttcgat3781 ccgagagagc tccgcaagga gacctgcctg ctttacgaaa ttaattgggg gggccggcac3841 tccatttggc gacatacatc acagaacact aacaagcacg tcgaagtcaa cttcatcgag3901 aagttcacga cagaaagata tttctgtccg aacacaaggt gcagcattac ctggtttctc3961 agctggagcc catgcggcga atgtagtagg gccatcactg aattcctgtc aaggtatccc4021 cacgtcactc tgtttattta catcgcaagg ctgtaccacc acgctgaccc ccgcaatcga4081 caaggcctgc gggatttgat ctcttcaggt gtgactatcc aaattatgac tgagcaggag4141 tcaggatact gctggagaaa ctttgtgaat tatagcccga gtaatgaagc ccactggcct服务时间:24小时在线客服
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