• 规格：20μl质粒
• 原核抗性：氯霉素Chl
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           The pETcoco expression vectors are designed to allow “on command” amplification of vector copy number from single copy (1–2 plasmid copies per cell) to medium copy (20– 50 per cell). The vector is maintained in the single copy state by utilizing the oriS and repE elements of the F episome together with the parABC partition determinants. The medium copy state is obtained by employing the RK-2 derived trfA replicator acting at oriV. The switch from single copy to medium copy status is achieved by inducing the expression of the trfA gene with arabinose. The dual replicon nature of these vectors promotes enhanced stability of recombinant plasmids and extremely low basal expression levels in the single copy state, while allowing robust target protein expression typical of the pET vectors. Unique restriction sites are shown on the circle map (below). The sequence is numbered relative to the start of T7 transcription at +1.
• 质粒图谱1： png/1-1G126133614948.png

质粒序列： 点击查看序列

       1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
      61 gagatataag catgcatcac catcaccatc acggtgctag caaagaaacc gctgctgcga
     121 aatttgaacg ccagcacatg gactcgccac cgccaactgg tgatgacgac gacaagagcc
     181 cgggcgatcg catcgatgac gtccaggcgc gccacgtgcg tacggtttcg aagcttgcgg
     241 ccgcaagcca gccagaactc gctccggaag atcccgagga tctttaatta acctcaggat
     301 ttaaataacc taggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa
     361 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc
     421 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg
     481 attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     721 tcacggccgc cgcaatgctt gcataatgtg gcctgtcaaa tggacgaagc agggattctg
     781 caaaccctat gctactccgt caagccgtca attgtctgat tcgttaccaa ttatgacaac
     841 ttgacggcta catcattcac tttttcttca caaccggcac gaaactcgct cgggctggcc
     901 ccggtgcatt ttttaaatac tcgcgagaaa tagagttgat cgtcaaaacc aacattgcga
     961 ccgacggtgg cgataggcat ccgggtagtg ctcaaaagca gcttcgcctg gctgatacgt
    1021 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga
    1081 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc
    1141 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg cggatggagc
    1201 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc
    1261 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg
    1321 aaatgcggct ggtgcgcttc atccgggcga aagaaacccg tattggcaaa tattgacggc
    1381 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat
    1441 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata
    1501 tcacccggtc ggcagacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg
    1561 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa
    1621 ccgttggcct caatcggcgt taaacccgcc accagatggg cgttaaacga gtatcccggc
    1681 agcaggggat cattttgcgc ttcagccata cttttcatac tcccaccatt cagagaagaa
    1741 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg
    1801 ctaacccaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc
    1861 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
    1921 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
    1981 acctgacgct ttttatcgca actctctact gtttctccat acccgttttt ttggatggag
    2041 tgaaaggagg aattcggggc aatcccgcaa ggagggtgaa tgaatcggac gtttgaccgg
    2101 aaggcataca ggcaagaact gatcgacgcg gggttttccg ccgaggatgc cgaaaccatc
    2161 gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc agtccgtcgg ctcgatggtc
    2221 cagcaagcta cggccaagat cgagcgcgac agcgtgcaac tggctccccc tgccctgccc
    2281 gcgccatcgg ccgccgtgga gcgttcgcgt cgtctcgaac aggaggcggc aggtttggcg
    2341 aagtcgatga ccatcgacac gcgaggaact atgacgacca agaagcgaaa aaccgccggc
    2401 gaggacctgg caaaacaggt cagcgaggcc aagcaggccg cgttgctgaa acacacgaag
    2461 cagcagatca aggaaatgca gctttccttg ttcgatattg cgccgtggcc ggacacgatg
    2521 cgagcgatgc caaacgacac ggcccgctct gccctgttca ccacgcgcaa caagaaaatc
    2581 ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca acaaggacgt gaagatcacc
    2641 tacaccggcg tcgagctgcg ggccgacgat gacgaactgg tgtggctgca ggtgttggag
    2701 tacgcgaagc gcacccctat cggcgagccg atcaccttca cgttctacga gctttgccag
    2761 gacctgggct ggtcgatcaa tggccggtat tacacgaagg ccgaggaatg cctgtcgcgc
    2821 ctacaggcga cggcgatgga cttcacgtcc gaccgcgttg ggcacctgga atcggtgtcg
    2881 ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa cgtcccgttg ccaggtcctg
    2941 atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact acacgaaatt catatgggag
    3001 aagtaccgca agctgtcgcc gacggcccga cggatgttcg actatttcag ctcgcaccgg
    3061 gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt gcggatcgga ttccacccgc
    3121 gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag agttgcgagg cagcggcctg
    3181 gtggaacacg cctgggtcaa tgatgacctg gtgcattgca aacgctaggg ccttgtgggg
    3241 tcagttccgg ctgggggttc agcagccacc gggccgtcga ccaattctca tgtttgacag
    3301 cttatcatcg aatttctgcc attcatccgc ttattatcac ttattcaggc gtagcaacca
    3361 ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg ccactcatcg
    3421 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
    3481 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
    3541 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
    3601 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
    3661 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
    3721 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
    3781 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat
    3841 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
    3901 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
    3961 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
    4021 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
    4081 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
    4141 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
    4201 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
    4261 tcgcgataag ctcatggagc ggcgtaaccg tcgcacagga aggacagaga aagcgcggat
    4321 ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc
    4381 tgctgacggt gtgacgttct ctgttccggt cacaccacat acgttccgcc attcctatgc
    4441 gatgcacatg ctgtatgccg gtataccgct gaaagttctg caaagcctga tgggacataa
    4501 gtccatcagt tcaacggaag tctacacgaa ggtttttgcg ctggatgtgg ctgcccggca
    4561 ccgggtgcag tttgcgatgc cggagtctga tgcggttgcg atgctgaaac aattatcctg
    4621 agaataaatg ccttggcctt tatatggaaa tgtggaactg agtggatatg ctgtttttgt
    4681 ctgttaaaca gagaagctgg ctgttatcca ctgagaagcg aacgaaacag tcgggaaaat
    4741 ctcccattat cgtagagatc cgcattatta atctcaggag cctgtgtagc gtttatagga
    4801 agtagtgttc tgtcatgatg cctgcaagcg gtaacgaaaa cgatttgaat atgccttcag
    4861 gaacaataga aatcttcgtg cggtgttacg ttgaagtgga gcggattatg tcagcaatgg
    4921 acagaacaac ctaatgaaca cagaaccatg atgtggtctg tccttttaca gccagtagtg
    4981 ctcgccgcag tcgagcgaca gggcgaagcc ctcgagatcc cctgccattt ttggggtgag
    5041 gccgttcgcg gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcggccgg
    5101 gagggttcga gaaggggggg cacccccctt cggcgtgcgc ggtcacgcgc acagggcgca
    5161 gccctggtta aaaacaaggt ttataaatat tggtttaaaa gcaggttaaa agacaggtta
    5221 gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg gattttctgc ctgtggacag
    5281 cccctcaaat gtcaataggt gcgcccctca tctgtcagca ctctgcccct caagtgtcaa
    5341 ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag tgtcaatacc gcagggcact
    5401 tatccccagg cttgtccaca tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc
    5461 gatttgcgag gctggccagc tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt
    5521 caacgccgcg ccgggtgagt cggcccctca agtgtcaacg tccgcccctc atctgtcagt
    5581 gagggccaag ttttccgcga ggtatccaca acgccggcgg ccggccgcgg tgtctcgcac
    5641 acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg
    5701 cgagggcaac cagcccggtg agcgtcggaa aggggaattt cgagtgagcg aggaagcacc
    5761 agggaacagc acttatatat tctgcttaca cacgatgcct gaaaaaactt cccttggggt
    5821 tatccactta tccacgggga tatttttata attatttttt ttatagtttt tagatcttct
    5881 tttttagagc gccttgtagg cctttatcca tgctggttct agagaaggtg ttgtgacaaa
    5941 ttgccctttc agtgtgacaa atcaccctca aatgacagtc ctgtctgtga caaattgccc
    6001 ttaaccctgt gacaaattgc cctcagaaga agctgttttt tcacaaagtt atccctgctt
    6061 attgactctt ttttatttag tgtgacaatc taaaaacttg tcacacttca catggatctg
    6121 tcatggcgga aacagcggtt atcaatcaca agaaacgtaa aaatagcccg cgaatcgtcc
    6181 agtcaaacga cctcactgag gcggcatata gtctctcccg ggatcaaaaa cgtatgctgt
    6241 atctgttcgt tgaccagatc agaaaatctg atggcaccct acaggaacat gacggtatct
    6301 gcgagatcca tgttgctaaa tatgctgaaa tattcggatt gacctctgcg gaagccagta
    6361 aggatatacg gcaggcattg aagagtttcg cggggaagga agtggttttt tatcgccctg
    6421 aagaggatgc cggcgatgaa aaaggctatg aatcttttcc ttggtttatc aaacgtgcgc
    6481 acagtccatc cagagggctt tacagtgtac atatcaaccc atatctcatt cccttcttta
    6541 tcgggttaca gaaccggttt acgcagtttc ggcttagtga aacaaaagaa atcaccaatc
    6601 cgtatgccat gcgtttatac gaatccctgt gtcagtatcg taagccggat ggctcaggca
    6661 tcgtctctct gaaaatcgac tggatcatag agcgttacca gctgcctcaa agttaccagc
    6721 gtatgcctga cttccgccgc cgcttcctgc aggtctgtgt taatgagatc aacagcagaa
    6781 ctccaatgcg cctctcatac attgagaaaa agaaaggccg ccagacgact catatcgtat
    6841 tttccttccg cgatatcact tccatgacga caggatagtc tgagggttat ctgtcacaga
    6901 tttgagggtg gttcgtcaca tttgttctga cctactgagg gtaatttgtc acagttttgc
    6961 tgtttccttc agcctgcatg gattttctca tactttttga actgtaattt ttaaggaagc
    7021 caaatttgag ggcagtttgt cacagttgat ttccttctct ttcccttcgt catgtgacct
    7081 gatatcgggg gttagttcgt catcattgat gagggttgat tatcacagtt tattactctg
    7141 aattggctat ccgcgtgtgt acctctacct ggagtttttc ccacggtgga tatttcttct
    7201 tgcgctgagc gtaagagcta tctgacagaa cagttcttct ttgcttcctc gccagttcgc
    7261 tcgctatgct cggttacacg gctgcggcga gcgctagtga taataagtga ctgaggtatg
    7321 tgctcttctt atctcctttt gtagtgttgc tcttatttta aacaactttg cggttttttg
    7381 atgactttgc gattttgttg ttgctttgca gtaaattgca agatttaata aaaaaacgca
    7441 aagcaatgat taaaggatgt tcagaatgaa actcatggaa acacttaacc agtgcataaa
    7501 cgctggtcat gaaatgacga aggctatcgc cattgcacag tttaatgatg acagcccgga
    7561 agcgaggaaa ataacccggc gctggagaat aggtgaagca gcggatttag ttggggtttc
    7621 ttctcaggct atcagagatg ccgagaaagc agggcgacta ccgcacccgg atatggaaat
    7681 tcgaggacgg gttgagcaac gtgttggtta tacaattgaa caaattaatc atatgcgtga
    7741 tgtgtttggt acgcgattgc gacgtgctga agacgtattt ccaccggtga tcggggttgc
    7801 tgcccataaa ggtggcgttt acaaaacctc agtttctgtt catcttgctc aggatctggc
    7861 tctgaagggg ctacgtgttt tgctcgtgga aggtaacgac ccccagggaa cagcctcaat
    7921 gtatcacgga tgggtaccag atcttcatat tcatgcagaa gacactctcc tgcctttcta
    7981 tcttggggaa aaggacgatg tcacttatgc aataaagccc acttgctggc cggggcttga
    8041 cattattcct tcctgtctgg ctctgcaccg tattgaaact gagttaatgg gcaaatttga
    8101 tgaaggtaaa ctgcccaccg atccacacct gatgctccga ctggccattg aaactgttgc
    8161 tcatgactat gatgtcatag ttattgacag cgcgcctaac ctgggtatcg gcacgattaa
    8221 tgtcgtatgt gctgctgatg tgctgattgt tcccacgcct gctgagttgt ttgactacac
    8281 ctccgcactg cagtttttcg atatgcttcg tgatctgctc aagaacgttg atcttaaagg
    8341 gttcgagcct gatgtacgta ttttgcttac caaatacagc aatagtaatg gctctcagtc
    8401 cccgtggatg gaggagcaaa ttcgggatgc ctggggaagc atggttctaa aaaatgttgt
    8461 acgtgaaacg gatgaagttg gtaaaggtca gatccggatg agaactgttt ttgaacaggc
    8521 cattgatcaa cgctcttcaa ctggtgcctg gagaaatgct ctttctattt gggaacctgt
    8581 ctgcaatgaa attttcgatc gtctgattaa accacgctgg gagattagat aatgaagcgt
    8641 gcgcctgtta ttccaaaaca tacgctcaat actcaaccgg ttgaagatac ttcgttatcg
    8701 acaccagctg ccccgatggt ggattcgtta attgcgcgcg taggagtaat ggctcgcggt
    8761 aatgccatta ctttgcctgt atgtggtcgg gatgtgaagt ttactcttga agtgctccgg
    8821 ggtgatagtg ttgagaagac ctctcgggta tggtcaggta atgaacgtga ccaggagctg
    8881 cttactgagg acgcactgga tgatctcatc ccttcttttc tactgactgg tcaacagaca
    8941 ccggcgttcg gtcgaagagt atctggtgtc atagaaattg ccgatgggag tcgccgtcgt
    9001 aaagctgctg cacttaccga aagtgattat cgtgttctgg ttggcgagct ggatgatgag
    9061 cagatggctg cattatccag attgggtaac gattatcgcc caacaagtgc ttatgaacgt
    9121 ggtcagcgtt atgcaagccg attgcagaat gaatttgctg gaaatatttc tgcgctggct
    9181 gatgcggaaa atatttcacg taagattatt acccgctgta tcaacaccgc caaattgcct
    9241 aaatcagttg ttgctctttt ttctcacccc ggtgaactat ctgcccggtc aggtgatgca
    9301 cttcaaaaag cctttacaga taaagaggaa ttacttaagc agcaggcatc taaccttcat
    9361 gagcagaaaa aagctggggt gatatttgaa gctgaagaag ttatcactct tttaacttct
    9421 gtgcttaaaa cgtcatctgc atcaagaact agtttaagct cacgacatca gtttgctcct
    9481 ggagcgacag tattgtataa gggcgataaa atggtgctta acctggacag gtctcgtgtt
    9541 ccaactgagt gtatagagaa aattgaggcc attcttaagg aacttgaaaa gccagcaccc
    9601 tgatgcgacc acgttttagt ctacgtttat ctgtctttac ttaatgtcct ttgttacagg
    9661 ccagaaagca taactggcct gaatattctc tctgggccca ctgttccact tgtatcgtcg
    9721 gtctgataat cagactggga ccacggtccc actcgtatcg tcggtctgat tattagtctg
    9781 ggaccacggt cccactcgta tcgtcggtct gattattagt ctgggaccac ggtcccactc
    9841 gtatcgtcgg tctgataatc agactgggac cacggtccca ctcgtatcgt cggtctgatt
    9901 attagtctgg gaccatggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg
    9961 gtcccactcg tatcgtcggt ctgattatta gtctggaacc acggtcccac tcgtatcgtc
   10021 ggtctgatta ttagtctggg accacggtcc cactcgtatc gtcggtctga ttattagtct
   10081 gggaccacga tcccactcgt gttgtcggtc tgattatcgg tctgggacca cggtcccact
   10141 tgtattgtcg atcagactat cagcgtgaga ctacgattcc atcaatgcct gtcaagggca
   10201 agtattgaca tgtcgtcgta acctgtagaa cggagtaacc tcggtgtgcg gttgtatgcc
   10261 tgctgtggat tgctgctgtg tcctgcttat ccacaacatt ttgcgcacgg ttatgtggac
   10321 aaaatacctg gttacccagg ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg
   10381 tgtcgctgtc gacgagctcg cgagctcgga catgaggttg ccccgtattc agtgtcgctg
   10441 atttgtattg tctgaagttg tttttacgtt aagttgatgc agatcaatta atacgatacc
   10501 tgcgtcataa ttgattattt gacgtggttt gatggcctcc acgcacgttg tgatatgtag
   10561 atgataatca ttatcacttt acgggtcctt tccggtgatc cgacaggtta cggggcggcg
   10621 acctcgcggg ttttcgctat ttatgaaaat tttccggttt aaggcgtttc cgttcttctt
   10681 cgtcataact taatgttttt atttaaaata ccctctgaaa agaaaggaaa cgacaggtgc
   10741 tgaaagcgag ctttttggcc tctgtcgttt cctttctctg tttttgtccg tggaatgaac
   10801 aatggaagtc cgagctcatc gctaataact tcgtatagca tacattatac gaagttatat
   10861 tcgatgcggc ctagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct
   10921 caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt
   10981 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
   11041 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca
   11101 gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc
   11161 ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga
   11221 tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc
   11281 gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca
   11341 gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca
   11401 tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt
   11461 tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg
   11521 cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat
   11581 gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa
   11641 cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga
   11701 tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc
   11761 cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa
   11821 tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca
   11881 gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg
   11941 ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca
   12001 cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg
   12061 gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa
   12121 aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc
   12181 attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt
   12241 gcatgtaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
   12301 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
   12361 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
   12421 gcgtagagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac tata