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- 规格：20μl质粒
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：大肠杆菌DH5α
- 质粒简介： pYES2-NTA质粒
- 质粒图谱1： png/1-1G126114132231.png
质粒序列： 点击查看序列LOCUS Exported 6038 bp ds-DNA circular SYN 10-NOV-2017DEFINITION synthetic circular DNAREFERENCE 1 (bases 1 to 6038)FEATURES Location/Qualifierssource 1..6038/organism="synthetic DNA construct"/mol_type="other DNA"promoter 2..443/gene="S. cerevisiae GAL1"/label=GAL1 promoter/note="inducible promoter, regulated by Gal4"protein_bind 2..119/label=UAS/bound_moiety="Gal4"/note="upstream activating sequence mediatingGal4-dependent induction"promoter 475..493/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"CDS 522..539/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"CDS 543..575/codon_start=1/product="leader peptide from bacteriophage T7 gene 10"/label=T7 tag (gene 10 leader)/note="promotes efficient translation in E. coli"/translation="MASMTGGQQMG"CDS 579..602/codon_start=1/product="Xpress(TM) epitope tag, including an enterokinaserecognition and cleavage site"/label=Xpress(TM) tag/translation="DLYDDDDK"CDS 682..723/codon_start=1/product="epitope tag from simian virus 5"/label=V5 tag/translation="GKPIPNPLLGLDST"CDS 733..750/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"terminator 784..1031/gene="S. cerevisiae CYC1"/label=CYC1 terminator/note="transcription terminator for CYC1"rep_origin complement(1279..1867)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(2038..2898)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"CDS complement(2994..3797)/codon_start=1/gene="S. cerevisiae URA3"/product="orotidine-5'-phosphate decarboxylase, requiredfor uracil biosynthesis"/label=URA3/note="yeast auxotrophic marker, counterselectable with5-fluoroorotic acid (5-FOA)"/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"promoter complement(3798..4013)/gene="S. cerevisiae URA3"/label=URA3 promoterrep_origin 4617..5497/label=2u ori/note="yeast 2u plasmid origin of replication"rep_origin complement(5566..6021)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"ORIGIN1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac481 gactcactat agggaatatt aagcttacca tggggggttc tcatcatcat catcatcatg541 gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata601 aggtacctag gatccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc661 gagtctagag ggcccttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct721 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc ctagagggcc781 gcatcatgta attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa841 ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta901 tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg961 tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg1021 ctttaatttg caagctgcgg ccctgcatta atgaatcggc caacgcgcgg ggagaggcgg1081 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg1141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg1201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aagcccagga accgtaaaaa1261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg1321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc1381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc1441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc1501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg1561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc1621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga1681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc1741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac1801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg1861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc1921 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa1981 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta2041 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt2101 tgcctgactc cccgtcgtgt agataactac gatacgggag cgcttaccat ctggccccag2161 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca2221 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc2281 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt2341 tgttggcatt gctacaggca tcgtggtgtc actctcgtcg tttggtatgg cttcattcag2401 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt2461 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat2521 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt2581 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc2641 ttgcccggcg tcaatacggg ataatagtgt atcacatagc agaactttaa aagtgctcat2701 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag2761 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt2821 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg2881 gaaatgttga atactcatac tcttcctttt tcaatgggta ataactgata taattaaatt2941 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt3001 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct3061 accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct3121 gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct3181 aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct3241 ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc3301 ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg3361 cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg3421 cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca3481 gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa3541 aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca3601 gtcaagatat ccacatgtgt ttttagtaaa caaattt
