• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：潮霉素Hyg
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：            pCEP4-tat是一个逆转录病毒tat表达包装质粒。
• 质粒图谱1： png/1-1G210194439A4.png

质粒序列： 点击查看序列

LOCUS       Exported               11625 bp ds-DNA     circular SYN 10-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..11625

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        4..383

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        384..587

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             664..969

                     /codon_start=1

                     /label=tat

                     /translation="MEPVDPNLEPWKHPGSQPRTACNNCYCKKCCFHCYACFTRKGLGI

                     SYGRKKRRQRRRAPQDSQTHQASLSKQPASQSRGDPTGPTESKKKVERETETDPFD"

     polyA_signal    976..1110

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     polyA_signal    2111..2245

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      2968..4757

                     /label=oriP

                     /note="Epstein-Barr virus oriP replication origin (Yates et

                     al., 2000)"

     CDS             complement(5059..6984)

                     /codon_start=1

                     /product="Epstein-Barr nuclear antigen 1, also known as 

                     EBNA-1"

                     /label=EBNA1

                     /note="crucial for latent viral infection, and for episomal

                     amplification of vectors containing the oriP origin (Okita 

                     et al., 2013)"

                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR

                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG

                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA

                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG

                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG

                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG

                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP

                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR

                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL

                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK

                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE

                     GEEGQE"

     promoter        7505..7609

                     /gene="bla"

                     /label=AmpR promoter

     CDS             7610..8470

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      8641..9229

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     promoter        9660..9805

                     /label=HSV TK promoter

                     /note="herpes simplex virus thymidine kinase promoter"

     CDS             9848..10885

                     /codon_start=1

                     /product="hygromycin B phosphotransferase"

                     /label=HygR

                     /note="confers resistance to hygromycin"

                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG

                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP

                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ

                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF

                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG

                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE

                     MGEAN"

     polyA_signal    10927..10975

                     /label=HSV TK poly(A) signal

                     /note="herpes simplex virus thymidine kinase 

                     polyadenylation signal (Cole and Stacy, 1985)"

ORIGIN

        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata

       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc

      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag

      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac

      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg

      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg

      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat

      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt

      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc

      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc

      601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctcgagcc

      661 accatggagc cagtagatcc taatctagag ccctggaagc atccaggaag tcagcctagg

      721 actgcttgta acaattgcta ttgtaaaaag tgttgctttc attgctacgc gtgtttcaca

      781 agaaaaggct taggcatctc ctatggcagg aagaagcgga gacagcgacg aagagctcct

      841 caggacagtc agactcatca agcttctcta tcaaagcaac ccgcctccca gtcccgaggg

      901 gacccgacag ggcccacgga atcgaagaag aaggtggaga gagagacaga gacagatccg

      961 ttcgattagc atgcagatcc agacatgata agatacattg atgagtttgg acaaaccaca

     1021 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt

     1081 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt

     1141 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt

     1201 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac

     1261 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag

     1321 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc

     1381 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct

     1441 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg

     1501 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat

     1561 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt

     1621 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt

     1681 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac

     1741 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag

     1801 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta

     1861 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca

     1921 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca

     1981 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt

     2041 agtcacaagg gcagtggctg aagatcaagg agcgggcagg gaatctcctg aatctcggcc

     2101 ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag

     2161 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac

     2221 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt

     2281 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc

     2341 tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg

     2401 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt

     2461 caggcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat

     2521 cgatgccccg ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg

     2581 gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca

     2641 tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata

     2701 aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc

     2761 tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa

     2821 tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc

     2881 aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt

     2941 gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat

     3001 atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta

     3061 tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg

     3121 tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca

     3181 agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc

     3241 ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg

     3301 tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt

     3361 tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa

     3421 tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg

     3481 acaagccgta aagactggat gtccatctca cacgaattta tggctatggg caacacataa

     3541 tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg

     3601 tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag

     3661 cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg

     3721 gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg