• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：潮霉素Hyg
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：              2C::tdTomato Reporter是一个哺乳细胞小鼠启动子红色荧光蛋白报告质粒。
• 质粒图谱1： png/1-1G221134P0362.png

质粒序列： 点击查看序列

LOCUS       Exported                7132 bp ds-DNA     circular SYN 21-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..7132

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    265..994

                     /label=2C Promoter (2-cell stage promoter)

     CDS             1067..2497

                     /codon_start=1

                     /product="tandem dimeric (pseudo-monomeric) derivative of 

                     DsRed (Shaner et al., 2004)"

                     /label=tdTomato

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEDVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA

                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG

                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH

                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH

                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY

                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM

                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV

                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE

                     RSEGRHHLFLYGMDELYK"

     polyA_signal    2591..2815

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      2861..3289

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        3303..3632

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      3483..3618

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             3681..4706

                     /codon_start=1

                     /gene="aph(4)-Ia"

                     /product="aminoglycoside phosphotransferase from E. coli"

                     /label=HygR

                     /note="confers resistance to hygromycin"

                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG

                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP

                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ

                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF

                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG

                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK

                     E"

     polyA_signal    4836..4957

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(5408..5996)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6167..7027)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7028..7132)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta

       61 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct

      121 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa

      181 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc

      241 gatgtacggg ccagatatac gcgtgctagc tgtagtggtt attcctggtt gtcaacttga

      301 caatatttgg aatgaactac agtccggaat tggaaggctc accagtgacc cttatctgga

      361 ggcttggaga tccttatctg gatcttggtt tgaagatctt gagccatagt ggctatggat

      421 tccagaagat tgaatctccg agtttaagga acacaccttt aatctgggct acgcctttca

      481 tctgggatta aaggtgtggt ggaacacacc tttaatctgg gctacacctt ctgctggaga

      541 caatataagg acattggaag aagggagtct agctcttgct cctgctcctt cgcctgcttg

      601 ctgcgtgaga ctgagtaact gctagatcct tggacttcca ttcacagctg tgactgaacg

      661 attgttggga attgagctgc cgactgtaag tcatcaataa attcctttat tatctagaga

      721 ctatccataa gttctgtgac tctagagaac cctgactaat acagaagttg gtaccaggag

      781 tggttctaga gtaacagaag tacaaggatg aatcttttaa aattctggaa ttggcttgtt

      841 gatccaccag cactttcaac tattgaaacc tctccagatt ctctccctcc tgggagctca

      901 gagaattttg aagacccatg gttgaaacta tattccgaac ttaaagaagc taatgccctt

      961 gattttctta atgaattagg tgattcagtg cacaaagctt ggtaccgagc tcggatccac

     1021 tagtaacggc cgccagtgtg ctggaattcg cccttgtctg tctagaatgg tgagcaaggg

     1081 cgaggacgtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg

     1141 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc

     1201 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca

     1261 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa

     1321 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct

     1381 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat

     1441 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga

     1501 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc

     1561 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa

     1621 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca

     1681 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt

     1741 cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga

     1801 caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat

     1861 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca

     1921 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc

     1981 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta

     2041 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg

     2101 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt

     2161 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg

     2221 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca

     2281 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat

     2341 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac

     2401 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca

     2461 cctgttcctg tacggcatgg acgagctgta caagtaatct agacccccaa gggcgaattc

     2521 tgcagatatc catcacactg gcggccgctc gagtctagag ggcccgttta aacccgctga

     2581 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct

     2641 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca

     2701 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag

     2761 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct

     2821 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca

     2881 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta

     2941 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt

     3001 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac

     3061 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt

     3121 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga

     3181 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg

     3241 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga

     3301 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa

     3361 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca

     3421 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc

     3481 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt

     3541 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag

     3601 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt

     3661 tcggatctga tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt

     3721 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat

     3781 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg

     3841 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga

     3901 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc

     3961 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc

     4021 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg

     4081 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga

     4141 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg

     4201 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc

     4261 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg

     4321 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct

     4381 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc

     4441 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac

     4501 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg

     4561 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg

     4621 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca

&nb