- 商品货号:FH1704
- 规格:10μl质粒
- 原核抗性:氨苄青霉素Amp
- 筛选标记:博来霉素Zeo
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: pcDNA4-V5-HisA是一个哺乳细胞pCDNA系列表达质粒。
- 质粒图谱1: png/1-1G2211031414J.png
质粒序列: 点击查看序列LOCUS Exported 5092 bp ds-DNA circular SYN 21-DEC-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..5092/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 235..614/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 615..818/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"promoter 863..881/label=T7 promoter/note="promoter for bacteriophage T7 RNA polymerase"CDS 1009..1050/codon_start=1/product="epitope tag from simian virus 5"/label=V5 tag/translation="GKPIPNPLLGLDST"CDS 1060..1077/codon_start=1/product="6xHis affinity tag"/label=6xHis/translation="HHHHHH"polyA_signal 1106..1330/label=bGH poly(A) signal/note="bovine growth hormone polyadenylation signal"rep_origin 1376..1804/direction=RIGHT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 1818..2147/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 1998..2133/label=SV40 ori/note="SV40 origin of replication"promoter 2195..2242/label=EM7 promoter/note="synthetic bacterial promoter "CDS 2261..2635/codon_start=1/gene="Sh ble from Streptoalloteichus hindustanus"/product="antibiotic-binding protein"/label=Zeo/note="confers resistance to bleomycin, phleomycin, andZeocin(TM)"/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQD"polyA_signal 2765..2886/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(3337..3925)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(4096..4956)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(4957..5061)/gene="bla"/label=AmpR promoterORIGIN1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc961 agatatccag cacagtggcg gccgctcgag tctagagggc ccttcgaagg taagcctatc1021 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga1081 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc1141 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa1201 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg1261 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg1321 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg1381 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca1441 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc1501 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct1561 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg1621 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc1681 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg1741 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg1801 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag1861 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag1921 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc1981 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc2041 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat2101 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag2161 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta2221 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc2281 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct2341 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac2401 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg2461 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg2521 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc2581 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt2641 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt2701 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca2761 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt2821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt2881 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata2941 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag3001 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg3061 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca3121 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt4021 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta4081 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct4141 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg4201 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga4261 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt4321 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt4381 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt4441 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat4501 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc4561 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc&nbs
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