- 商品货号:FH1715
- 规格:10μl质粒
- 原核抗性:卡那霉素Kan
- 筛选标记:遗传霉素G418
- 克隆菌株:大肠杆菌DH5α
- 质粒简介: phU6-gRNA是一个哺乳细胞gRNA表达质粒。
- 质粒图谱1: png/1-1G214164636453.png
质粒序列: 点击查看序列LOCUS Exported 3515 bp ds-DNA circular SYN 30-NOV-2017DEFINITION synthetic circular DNAFEATURES Location/Qualifierssource 1..3515/organism="synthetic DNA construct"/mol_type="other DNA"rep_origin complement(3..458)/direction=LEFT/label=f1 ori/note="f1 bacteriophage origin of replication; arrowindicates direction of (+) strand synthesis"promoter 696..936/label=U6 promoter/note="RNA polymerase III promoter for human U6 snRNA"misc_RNA 963..1038/label=gRNA scaffold/note="guide RNA scaffold for the Streptococcus pyogenesCRISPR/Cas9 system"rep_origin complement(1535..2123)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(2200..2994)/codon_start=1/gene="aph(3')-II (or nptII)"/product="aminoglycoside phosphotransferase from Tn5"/label=NeoR/KanR/note="confers resistance to neomycin, kanamycin, and G418(Geneticin(R))"/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"promoter complement(3029..3386)/label=SV40 promoter/note="SV40 enhancer and early promoter"rep_origin 3043..3178/label=SV40 ori/note="SV40 origin of replication"promoter complement(3388..3492)/gene="bla"/label=AmpR promoterORIGIN1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg661 tctcacaggc ggatcgatcc aaggtcgggc aggaagaggg cctatttccc atgattcctt721 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa781 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg841 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt901 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggtct tcgagaagac961 ctgttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa1021 gtggcaccga gtcggtgctt tttttctccg ctgagcgtac tgagacgccg cggtggagct1081 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc1141 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca1201 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct1261 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac1321 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc1381 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt1441 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg1501 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg1561 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat1621 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta1681 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct1741 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc1801 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa1861 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg1921 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag1981 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt2041 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta2101 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc2161 agtggaacga aaactcacgt taagggattt tggtcatgat cagaagaact cgtcaagaag2221 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg2281 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg2341 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc2401 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg2461 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc2521 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg2581 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc2641 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc2701 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc2761 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc2821 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag2881 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag2941 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa3001 cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc taggcctcca aaaaagcctc3061 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa3121 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg3181 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc3241 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc3301 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat3361 tccacagctg gttctttccg cctcaggact cttccttttt caatattatt gaagcattta3421 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat3481 aggggttccg cgcacatttc cccgaaaagt gccac//
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