• 规格：20μl质粒
• 启动子：beta-Actin Promoter
• 复制子：pUC
• 原核抗性：氨苄青霉素Amp
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：              pActin-EGFP-beta-Actin (Homo)是一个哺乳细胞人源基因过表达质粒。
• 质粒图谱1： png/1-1G22009325A95.png

质粒序列： 点击查看序列

LOCUS       Exported                9339 bp ds-DNA     circular SYN 20-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..9339

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    320..2960

                     /label=beta-Actin Promoter (Homo)

     CDS             2971..3687

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL

                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD

                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK

                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL

                     EFVTAAGITLGMDELYK"

     CDS             3703..4830

                     /codon_start=1

                     /label=beta-Actin (Homo)

                     /translation="MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMV

                     GMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL

                     LTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTV

                     PIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALD

                     FEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNS

                     IMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVW

                     IGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"

     polyA_signal    4935..5159

                     /label=bGH poly(A) signal

                     /note="bovine growth hormone polyadenylation signal"

     rep_origin      5205..5633

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        5647..5977

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      5828..5963

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             6044..6838

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    7012..7133

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(7584..8172)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(8343..9203)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=Amp

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(9204..9308)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gacggatcgg gagatcttgg ccagctgaat ggaggcccag cggcaacaca ggtcctgcct

       61 ggggatcagg tctgctctgc accccacctt gctgcctgga gccgcccacc tgacaacctc

      121 tcatccctgc tctgtagatc cggtcccatc cccactgccc accccacccc cccagcactc

      181 cacccagttc aacgttccac gaacccccag aaccagccct catcaacagg cagcaagaag

      241 ggccccccgc ccatcgcccc acaacgccag ccgggtgaac tgtagcgttg gcaggtcctg

      301 aggcagctgg caagacgcct gcagctgaaa gatacaaggc cagggacagg acagtcccat

      361 ccccaggagg cagggagtat acaggctggg gaagtttgcc cttgcgtggg gtggtgatgg

      421 aggaggctca gcaagtcttc tggactgtga acctgtgtct gccactgtgt gctgggtggt

      481 ggtcatcttt cccaccaggc tgtggcctct gcaaccttca agggaggagc aggtcccatt

      541 ggctgagcac agccttgtac cgtgaactgg aacaagcagc ctccttcctg gccacaggtt

      601 ccatgtcctt atatggactc atctttgcct attgcgacac acactcaatg aacacctact

      661 acgcgctgca aagagccccg caggcctgag gtgcccccac ctcaccactc ttcctatttt

      721 tgtgtaaaaa tccagcttct tgtcaccacc tccaaggagg gggaggagga ggaaggcagg

      781 ttcctctagg ctgagccgaa tgcccctctg tggtcccacg ccactgatcg ctgcatgccc

      841 accacctggg tacacacagt ctgtgattcc cggagcagaa cggaccctgc ccacccggtc

      901 ttgtgtgcta ctcagtggac agacccaagg caagaaaggg tgacaaggac agggtcttcc

      961 caggctggct ttgagttcct agcaccgccc cgcccccaat cctctgtggc acatggagtc

     1021 ttggtcccca gagtccccca gcggcctcca gatggtctgg gagggcagtt cagctgtggc

     1081 tgcgcatagc agacatacaa cggacggtgg gcccagaccc aggctgtgta gacccagccc

     1141 ccccgccccg cagtgcctag gtcacccact aacgccccag gcctggtctt ggctgggcgt

     1201 gactgttacc ctcaaaagca ggcagctcca gggtaaaagg tgccctgccc tgtagagccc

     1261 accttccttc ccagggctgc ggctgggtag gtttgtagcc ttcatcacgg gccacctcca

     1321 gccactggac cgctggcccc tgccctgtcc tggggagtgt ggtcctgcga cttctaagtg

     1381 gccgcaagcc acctgactcc cccaacacca cactctacct ctcaagccca ggtctctccc

     1441 tagtgaccca cccagcacat ttagctagct gagccccaca gccagaggtc ctcaggccct

     1501 gctttcaggg cagttgctct gaagtcggca agggggagtg actgcctggc cactccatgc

     1561 cctccaagag ctccttctgc aggagcgtac agaacccagg gccctggcac ccgtgcagac

     1621 cctggcccac cccacctggg cgctcagtgc ccaagagatg tccacaccta ggatgtcccg

     1681 cggtgggtgg ggggcccgag agacgggcag gccgggggca ggcctggcca tgcggggccg

     1741 aaccgggcac tgcccagcgt ggggcgcggg ggccacggcg cgcgccccca gcccccgggc

     1801 ccagcacccc aaggcggcca acgccaaaac tctccctcct cctcttcctc aatctcgctc

     1861 tcgctctttt tttttttcgc aaaaggaggg gagagggggt aaaaaaatgc tgcactgtgc

     1921 ggcgaagccg gtgagtgagc ggcgcggggc caatcagcgt gcgccgttcc gaaagttgcc

     1981 ttttatggct cgagcggccg cggcggcgcc ctataaaacc cagcggcgcg acgcgccacc

     2041 accgccgaga ccgcgtccgc cccgcgagca cagagcctcg cctttgccga tccgccgccc

     2101 gtccacaccc gccgccaggt aagcccggcc agccgaccgg ggcatgcggc cgcggcccct

     2161 tcgcccgtgc agagccgccg tctgggccgc agcggggggc gcatgggggg ggaaccggac

     2221 cgccgtgggg ggcgcgggag aagcccctgg gcctccggag atgggggaca ccccacgcca

     2281 gttcggaggc gcgaggccgc gctcgggagg cgcgctccgg gggtgccgct ctcggggcgg

     2341 gggcaaccgg cggggtcttt gtctgagccg ggctcttgcc aatggggatc gcagggtggg

     2401 cgcggcgtag cccccgccag gcccggtggg ggctggggcg ccattgcgcg tgcgcgctgg

     2461 tcctttgggc gctaactgcg tgcgcgctgg gaattggcgc taattgcgcg tgcgcgctgg

     2521 gactcaaggc gctaattgcg cgtgcgttct ggggcccggg gtgccgcggc ctgggctggg

     2581 gcgaaggcgg gctcggccgg aaggggtggg gtcgccgcgg ctcccgggcg cttgcgcgca

     2641 cttcctgccc gagccgctgg ccgcccgagg gtgtggccgc tgcgtgcgcg cgcgccgacc

     2701 cggcgctgtt tgaaccgggc ggaggcgggg ctggcgcccg gttgggaggg ggttggggcc

     2761 tggcttcctg ccgcgcgccg cggggacgcc tccgaccagt gtttgccttt tatggtaata

     2821 acgcggccgg cccggcttcc tttgtcccca atctgggcgc gcgccggcgc cccctggcgg

     2881 cctaaggact cggcgcgccg gaagtggcca gggcgggggc gacctcggct cacagcgcgc

     2941 ccggctattc tcgcagctca agcttccacc atggtgagca agggcgagga gctgttcacc

     3001 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg

     3061 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc

     3121 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag

     3181 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc

     3241 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc

     3301 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac

     3361 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac

     3421 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac

     3481 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc

     3541 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa

     3601 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc

     3661 actctcggca tggacgagct gtacaaggga tccactagtg gcatggatga tgatatcgcc

     3721 gcgctcgtcg tcgacaacgg ctccggcatg tgcaaggccg gcttcgcggg cgacgatgcc

     3781 ccccgggccg tcttcccctc catcgtgggg cgccccaggc accagggcgt gatggtgggc

     3841 atgggtcaga aggattccta tgtgggcgac gaggcccaga gcaagagagg catcctcacc

     3901 ctgaagtacc ccatcgagca cggcatcgtc accaactggg acgacatgga gaaaatctgg

     3961 caccacacct tctacaatga gctgcgtgtg gctcccgagg agcaccccgt gctgctgacc

     4021 gaggcccccc tgaaccccaa ggccaaccgc gagaagatga cccagatcat gtttgagacc

     4081 ttcaacaccc cagccatgta cgttgctatc caggctgtgc tatccctgta cgcctctggc

     4141 cgtaccactg gcatcgtgat ggactccggt gacggggtca cccacactgt gcccatctac

     4201 gaggggtatg ccctccccca tgccatcctg cgtctggacc tggctggccg ggacctgact

     4261 gactacctca tgaagatcct caccgagcgc ggctacagct tcaccaccac ggccgagcgg

     4321 gaaatcgtgc gtgacattaa ggagaagctg tgctacgtcg ccctggactt cgagcaagag

     4381 atggccacgg ctgcttccag ctcctccctg gagaagagct acgagctgcc tgacggccag