• 规格：20μl质粒
• 启动子：CAG
• 复制子：pUC
• 终止子：β-globin poly(A) signal
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：        pHLmMBP-10是一个哺乳细胞蛋白表达质粒。
• 质粒图谱1： png/1-1G226114323296.png

质粒序列： 点击查看序列

LOCUS       Exported                5931 bp ds-DNA     circular SYN 26-DEC-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5931

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    4..1670

                     /label=CAG promoter

     regulatory      1758..1767

                     /regulatory_class="other"

                     /note="vertebrate consensus sequence for strong initiation 

                     of translation (Kozak, 1987)"

     CDS             1857..1880

                     /codon_start=1

                     /product="8xHis affinity tag"

                     /label=8xHis

                     /translation="HHHHHHHH"

     misc_feature    1881..2981

                     /label=MBP

     CDS             3015..3035

                     /codon_start=1

                     /product="tobacco etch virus (TEV) protease recognition and

                     cleavage site"

                     /label=TEV site

                     /translation="ENLYFQS"

     CDS             3066..3083

                     /codon_start=1

                     /product="6xHis affinity tag"

                     /label=6xHis

                     /translation="HHHHHH"

     CDS             3111..3134

                     /codon_start=1

                     /product="peptide that binds Strep-Tactin(R), an engineered

                     form of streptavidin"

                     /label=Strep-Tag II

                     /translation="WSHPQFEK"

     CDS             3153..3179

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /label=HA

                     /translation="YPYDVPDYA"

     polyA_site      3353..3408

                     /label=-beta-globin poly(A) signal

                     /note="rabbit beta-globin polyadenylation signal"

     rep_origin      complement(4181..4769)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4940..5800)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5801..5905)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata

       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc

      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag

      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac

      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg

      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg

      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc

      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca

      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg

      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt

      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc

      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc

      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc

      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag

      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg

      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg

      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc

     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg

     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc

     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc

     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg

     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct

     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg

     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc

     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt

     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg

     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg

     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca

     1681 acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attgcggccg tctcaggccg

     1741 aattcgtcga caagcttgcc accatgggga tccttcccag ccctgggatg cctgcgctgc

     1801 tctccctcgt gagccttctc tccgtgctgc tgatgggttg cgtagctgaa accggtcacc

     1861 atcaccatca ccatcaccat aaaaccgaag aaggtaaact ggtaatctgg attaacggcg

     1921 ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat accggaatta

     1981 aagtcaccgt tgagcatccc gataaactgg aagagaaatt cccacaggtt gcggcaactg

     2041 gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac gctcaatctg

     2101 gcctgttggc tgaaatcacc ccggccgcag cgttccagga caagctgtat ccgtttacct

     2161 gggatgccgt gcgctacaac ggcaagctga ttgcttaccc gatcgctgtt gaagcgttgt

     2221 ccctgattta taacaaggat ctgctgccga acccgccaaa aacctgggaa gagatcccgg

     2281 cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac ctgcaagaac

     2341 cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag tatgcagccg

     2401 gaaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg ggtctgacct

     2461 tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac tccatcgcag

     2521 aacatgcctt taatcatggc gaaacagcga tgaccatcaa cggcccgtgg gcatggtcca

     2581 acatcgacac cagcgcagtg aattatggtg taacggtgct gccgaccttc aagggtcaac

     2641 catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt ccgaacaaag

     2701 agctggcaaa agagttcctc gaaaactacc tgctgactga tgaaggtctg gaagcggtta

     2761 ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag ttggtgaaag

     2821 atccacgtgt tgccgccaca atggaaaacg cccagaaagg tgaaatcatg cccaacatcc

     2881 cgcagatgtc cgctttctgg tatgccgtgc gcactgcggt gattaacgcc gccagcggtc

     2941 gtcagactgt cgatgcagcc ctggcagccg cgcagactaa tatcaccaag ggcggaggga

     3001 gcggcggagg gagcgaaaac ctgtattttc agagcgcggc cgccgcgagc tcggctagcc

     3061 tcgagcacca tcaccatcac cattgataaa cgcgtttaat taaccccggg tggagccacc

     3121 cccagttcga gaagtgataa gatgatcaag gatatcccta cgacgtgccc gactacgcct

     3181 gataatctag aactagtatc gcgataattc actcctcagg tgcaggctgc ctatcagaag

     3241 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct

     3301 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg

     3361 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca

     3421 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca

     3481 tatgccatat gctggctgcc atgaacaaag gtggctataa agaggtcatc agtatatgaa

     3541 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt

     3601 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt

     3661 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct

     3721 cttatgaaga tccctcgacc tgcagcccaa gcttggcgta atcatggtca tagctgtttc

     3781 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt

     3841 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc

     3901 ccgctttcca gtcgggaaac ctgtcgtgcc agcggatcga tccgctgcat taatgaatcg

     3961 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg

     4021 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa

     4081 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc

     4141 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc

     4201 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat

     4261 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc

     4321 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct

     4381 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg

     4441 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc

     4501 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga

     4561 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa

     4621 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta

     4681 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc

     4741 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg

     4801 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga

     4861 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg

     4921 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct

     4981 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg

     5041 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc

     5101 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa

     5161 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc

     5221 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt

     5281 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc

     5341 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt

     5401 tggccgcag