• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：            pDGIEF是一个枯草杆菌整合分泌表达质粒。
• 质粒图谱1： png/1-1P103133353A1.png

质粒序列： 点击查看序列

LOCUS       Exported                8717 bp ds-DNA     circular SYN 10-AUG-2017

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..8717

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             complement(823..1905)

                     /codon_start=1

                     /gene="lacI"

                     /product="lac repressor"

                     /label=lacI

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL

                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV

                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH

                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA

                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC

                     YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR

                     ALADSLMQLARQVSRLESGQ"

     protein_bind    2514..2530

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     rep_origin      complement(6888..7476)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(7647..8507)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(8508..8612)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga

       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt

      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat

      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt

      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg

      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg

      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg

      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa

      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat

      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca

      601 catggaaaca cacaaattaa aaactggtct gatcggatcc gcggctagcc atggtcgacg

      661 cggccgcaaa attgaaaaaa tggtggaaac acttttttca atttttttgt tttattattt

      721 aatatttggg aaatattcat tctaattggt aatcagattt tagaaaacaa taaacccttg

      781 cataggggga tcggatccta actcacatta attgcgttgc gctcactgcc cgctttccag

      841 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt

      901 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt

      961 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag

     1021 caggcgaaaa tcctgtttga tggtggttga cggcgggata taacatgagc tgtcttcggt

     1081 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc

     1141 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc

     1201 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg

     1261 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag

     1321 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc

     1381 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat

     1441 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac

     1501 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc

     1561 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac

     1621 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg

     1681 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag

     1741 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc

     1801 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga

     1861 gacaccggca tactctgcga catcgtataa cgttactggt ttcatcaaaa tcgtctccct

     1921 ccgtttgaat atttgattga tcgtaaccag atgaagcact ctttccacta tccctacagt

     1981 gttatggctt gaacaatcac gaaacaataa ttggtacgta cgatctttca gccgactcaa

     2041 acatcaaatc ttacaaatgt agtctttgaa agtattacat atgtaagatt taaatgcaac

     2101 cgttttttcg gaaggaaatg atgacctcgt ttccaccgga attagcttgc atgcctaccc

     2161 aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa ctgttccttt

     2221 cttcgttgct cctcttgccc gccaggcgat actttttacc tgatcagcta acgctacgcc

     2281 atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt gcgttgtaca

     2341 aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca ggacaacagc

     2401 tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc

     2461 gcccatatcg ggtacgtatc ggcttaccat acctgcttcc tccttaagct taattgttat

     2521 ccgctcacaa ttccacacat tatgccacac cttgtagata aagtcaacaa ctttttgcaa

     2581 aatttttcag gaattttagc agaggttgtt ctggatgtag aacaaaacat ctttccgctc

     2641 ttgtgctgtt aggatatctt tcttggaagc taggtaggca agggctacct caaataaatc

     2701 ttcttcaggg tgcgctattt ttaaggtgcc tagtgaggtc ttgaccactt cacccataat

     2761 ttcagtgccg aatagtctgg actgggctgt gtagaattcc tgcagccctg gcgaatggcg

     2821 attttcgttc gtgaatacat gttataataa ctataactaa taacgtaacg tgactggcaa

     2881 gagatatttt taaaacaatg aataggttta cacttacttt agttttatgg aaatgaaaga

     2941 tcatatcata tataatctag aataaaatta actaaaataa ttattatcta gataaaaaat

     3001 ttagaagcca atgaaatcta taaataaact aaattaagtt tatttaatta acaactatgg

     3061 atataaaata ggtactaatc aaaatagtga ggaggatata tttgaataca tacgaacaaa

     3121 ttaataaagt gaaaaaaata cttcggaaac atttaaaaaa taaccttatt ggtacttaca

     3181 tgtttggatc aggagttgag agtggactaa aaccaaatag tgatcttgac tttttagtcg

     3241 tcgtatctga accattgaca gatcaaagta aagaaatact tatacaaaaa attagaccta

     3301 tttcaaaaaa aataggagat aaaagcaact tacgatatat tgaattaaca attattattc

     3361 agcaagaaat ggtaccgtgg aatcatcctc ccaaacaaga atttatttat ggagaatggt

     3421 tacaagagct ttatgaacaa ggatacattc ctcagaagga attaaattca gatttaacca

     3481 taatgcttta ccaagcaaaa cgaaaaaata aaagaatata cggaaattat gacttagagg

     3541 aattactacc tgatattcca ttttctgatg tgagaagagc cattatggat tcgtcagagg

     3601 aattaataga taattatcag gatgatgaaa ccaactctat attaacttta tgccgtatga

     3661 ttttaactat ggacacgggt aaaatcatac caaaagatat tgcgggaaat gcagtggctg

     3721 aatcttctcc attagaacat agggagagaa ttttgttagc agttcgtagt tatcttggag

     3781 agaatattga atggactaat gaaaatgtaa atttaactat aaactattta aataacagat

     3841 taaaaaaatt ataaaaaaat tgaaaaaatg gtggaaacac ttttttcaat ttttttgttt

     3901 tattatttaa tatttgggaa atattcattc taattggtaa tcagatttta gaaaacaata

     3961 aacccttgca tagggggatc tcgacatgga tgagcgatga tgatatccgt ttaggctggg

     4021 cggtgatagc ttctcgttca ggcagtacgc ctcttttctt ttccagacct gagggaggcg

     4081 gaaatggtgt gaggttcccg gggaaaagcc aaataggcga tcgcgggagt gctttatttg

     4141 aagatcaggc tatcactgcg gtcaatagat ttcacaatgt gatggctgga cagcctgagg

     4201 aactctcgaa cccgaatgga aacaaccaga tatttatgaa tcagcgcggc tcacatggcg

     4261 ttgtgctggc aaatgcaggt tcatcctctg tctctatcaa tacggcaaca aaattgcctg

     4321 atggcaggta tgacaataaa gctggagcgg gttcatttca agtgaacgat ggtaaactga

     4381 caggcacgat caatgccagg tctgtagctg tgctttatcc tgatgatatt gcaaaagcgc

     4441 ctcatgtttt ccttgagaat tacaaaacag gtgtaacaca ttctttcaat gatcaactga

     4501 cgattacctt gcgtgcagat gcgaatacaa caaaagccgt ttatcaaatc aataatggac

     4561 cagacgacag gcgtttaagg atggagatca attcacaatc ggaaaaggag atccaatttg

     4621 gcaaaacata caccatcatg ttaaaaggaa cgaacagtga tggtgtaacg aggaccgaga

     4681 aatacagttt tgttaaaaga gatccagcgt cggccaaaac catcggctat caaaatccga

     4741 atcattggag ccaggtaaat gcttatatct ataaacatga tgggagccga gtaattgaat

     4801 tgaccggatc ttggcctgga aaaccaatga ctaaaaatgc agacggaatt tacacgctga

     4861 cgctgcctgc ggacacggat acaaccaacg caaaagtgat ttttaataat ggcagcgccc

     4921 aagtgcccgg tcagaatcag cctggctttg attacgtgct aaatggttta tataatgact

     4981 cgggcttaag cggttctctt ccccattgag ggcaaggcta gacgggactt accgaaagaa

     5041 accatcaatg atggtttctt ttttgttcat aaatcagaca aaacttttct cttgcaaaag

     5101 tttgtgaagt gttgcacaat ataaatgtga aatacttcac aaacaaaaag acatcaaaga

     5161 gaaacatacc ctgcaaggat gctgatattg tctgcatttg cgccggagca aaccaaaaac

     5221 ctggtgagac acgccttgaa ttagtagaaa agaacttgaa gattttcaaa ggcatcgtta

     5281 gtgaagtcat ggcgagcgga tttgacggca ttttcttagt cgcgacgcga ggctggatgg

     5341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca

     5401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc

     5461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg

     5521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc

     5581 ccgcgttgcg tcgcggtgca tggagccggg ccacctactg aagtggattt ctttaagagc

     5641 tccgataacc agaagcaata aaaaatcaaa tcggatttca ctatataatc tcactttatc

     5701 taagatgaat ccgatggaag catcctgttt tctctcaatt tttttatcta aaacccagcg

     5761 ttcgatgctt ctttgagcga acgatcaaaa ataagtgcct tcccatcaaa aaaatattct

     5821 caacataaaa aactttgtgt aatacttgta acgctacatg gagattaact caatctagag

     5881 ggtattaata atgatccaca gtagcgtaaa gcgttgggga aattcaccgg cggtgcggat

     5941 cccggctacg ttaatgcagg cgctcaatct gaatattgat gatgaagtga agattgacct

     6001 ggtggatggc aaattaatta ttgagccagt gcgtaaagag cccgtattta cgcttgctga

     6061 actggtcaac gacatcacgc cggaaaacct ccacgagaat atcgactggg gagagccgaa

     6121 agataaggaa gtctggtaaa tcgattatgt cttttgcgca gtcggcttaa accagttttc

     6181 gctggtgcga aaaaagagtg tcttgtgaca cctaaattca aaatctatcg gt