• 规格：20μl质粒
• 启动子：T7
• 复制子：pBR322
• 终止子：T7 ter
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：               pET11a-Hiv-1-Protease是一个病毒基因大肠杆菌表达质粒。
• 质粒图谱1： png/1-1P102115545950.png

质粒序列： 点击查看序列

LOCUS       Exported                5977 bp ds-DNA     circular SYN 02-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..5977

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        1..19

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     protein_bind    20..44

                     /label=lac operator

                     /bound_moiety="lac repressor encoded by lacI"

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

     CDS             89..424

                     /codon_start=1

                     /label=HIV-1

                     /translation="MADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVL

                     EEMNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQ

                     IGCTLNF"

     terminator      489..536

                     /label=T7 terminator

                     /note="transcription terminator for bacteriophage T7 RNA 

                     polymerase"

     promoter        852..956

                     /gene="bla"

                     /label=AmpR promoter

     CDS             957..1817

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      1988..2576

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3006..3197)

                     /codon_start=1

                     /gene="rop"

                     /product="Rop protein"

                     /label=rop

                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA

                     DELYRSCLARFGDDGENL"

     CDS             complement(4509..5591)

                     /codon_start=1

                     /gene="lacI"

                     /product="lac repressor"

                     /label=lacI

                     /note="The lac repressor binds to the lac operator to 

                     inhibit transcription in E. coli. This inhibition can be 

                     relieved by adding lactose or 

                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL

                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV

                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH

                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA

                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC

                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR

                     ALADSLMQLARQVSRLESGQ"

     promoter        complement(5592..5669)

                     /gene="lacI"

                     /label=lacI promoter

ORIGIN

        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt

       61 tgtttaactt taagaaggag atatacatat ggccgataga caaggaactg tatcctttag

      121 cttccctcag atcactcttt ggcagcgacc cctcgtcaca ataaagatag gggggcaatt

      181 aaaggaagct ctattagata caggagcaga tgatacagta ttagaagaaa tgaatttgcc

      241 aggaagatgg aaaccaaaaa tgataggggg aattggaggt tttatcaaag taagacagta

      301 tgatcagata ctcatagaaa tctgcggaca taaagctata ggtacagtat tagtaggacc

      361 tacacctgtc aacataattg gaagaaatct gttgactcag attggttgca ctttaaattt

      421 ttagggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg

      481 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga

      541 aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca taaccaagcc

      601 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt

      661 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt

      721 atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc

      781 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc

      841 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc

      901 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga

      961 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt

     1021 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag

     1081 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag

     1141 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg

     1201 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg

     1261 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca

     1321 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag

     1381 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc

     1441 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg

     1501 cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc

     1561 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg

     1621 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg

     1681 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga

     1741 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac

     1801 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa

     1861 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca

     1921 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag

     1981 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac

     2041 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa

     2101 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc

     2161 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag

     2221 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac

     2281 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc

     2341 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc

     2401 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca

     2461 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc

     2521 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg

     2581 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct

     2641 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata

     2701 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc

     2761 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg

     2821 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg

     2881 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga

     2941 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc

     3001 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca

     3061 tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg

     3121 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt

     3181 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg

     3241 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg

     3301 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa

     3361 atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc

     3421 cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt

     3481 tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac

     3541 gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca

     3601 gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc

     3661 cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac

     3721 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga

     3781 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg

     3841 tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg

     3901 cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg

     3961 tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa

     4021 gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc

     4081 cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga

     4141 acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct

     4201 cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc

     4261 cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga

     4321 aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca

     4381 taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg

     4441 ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg

     4501 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa

     4561 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc

     4621 accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc

     4681 aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc

     4741 gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca

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