×
![]()
- 规格：20μl质粒
- 原核抗性：氨苄青霉素Amp
- 克隆菌株：大肠杆菌DH5α
- 质粒图谱1： png/1-1P121192005936.png
质粒序列： 点击查看序列LOCUS Exported 7098 bp ds-DNA circular SYN 21-JAN-2018FEATURES Location/Qualifierssource 1..7098/organism="synthetic DNA construct"/mol_type="other DNA"enhancer 27..330/label=CMV enhancer/note="human cytomegalovirus immediate early enhancer"promoter 331..534/label=CMV promoter/note="human cytomegalovirus (CMV) immediate earlypromoter"intron 672..768/label=SV40 intron/note="modified SV40 intron with splice donor and acceptorsites"regulatory 845..854/regulatory_class="other"/note="vertebrate consensus sequence for strong initiationof translation (Kozak, 1987)"misc_feature 851..4189/label=BKCACDS 4199..4222/codon_start=1/product="FLAG(R) epitope tag, followed by an enterokinasecleavage site"/label=FLAG/translation="DYKDDDDK"polyA_signal 4237..4371/label=SV40 poly(A) signal/note="SV40 polyadenylation signal"rep_origin complement(4884..5472)/direction=LEFT/label=ori/note="high-copy-number ColE1/pMB1/pBR322/pUC origin ofreplication"CDS complement(5643..6503)/codon_start=1/gene="bla"/product="beta-lactamase"/label=AmpR/note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"promoter complement(6504..6608)/gene="bla"/label=AmpR promoterORIGIN1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccaggcc cgaattcggt841 cgacgccacc atggatgcgc tcatcatccc ggtgaccatg gaggtgccgt gcgacagccg901 gggccaacgc atgtggtggg ctttcctggc ctcctccatg gtgactttct tcgggggcct961 cttcatcatc ttgctctggc ggacgctcaa gtacctgtgg accgtgtgct gccactgcgg1021 gggcaagacg aaggaggccc agaagattaa caatggctca agccaggcgg atggcactct1081 caaaccagtg gatgaaaaag aggaggcagt ggccgccgag gtcggctgga tgacctccgt1141 gaaggactgg gcgggggtga tgatatccgc ccagacactg actggcagag tcctggttgt1201 cttagtcttt gctctcagca tcggtgcact tgtaatatac ttcatagatt catcaaaccc1261 aatagaatcc tgccagaatt tctacaaaga tttcacatta cagatcgaca tggctttcaa1321 cgtgttcttc cttctctact ttggcttgcg gtttattgca gccaacgata aattgtggtt1381 ctggctggaa gtgaactctg tagtggattt cttcacggtg ccccccgtgt ttgtgtctgt1441 gtacttaaac agaagttggc ttggtttgag atttttaaga gctctgagac tgatacagtt1501 ttcagaaatt ttgcagtttc tgaatattct taaaacaagt aattccatca agctggtgaa1561 tctgctctcc atatttatca gcacgtggct gactgcagcc gggttcatcc atttggtgga1621 gaattcaggg gacccatggg aaaatttcca aaacaaccag gctctcacct actgggaatg1681 tgtctattta ctcatggtca caatgtccac cgttggttat ggggatgttt atgcaaaaac1741 cacacttggg cgcctcttca tggtcttctt catcctcggg ggactggcca tgtttgccag1801 ctacgtccct gaaatcatag agttaatagg aaaccgcaag aaatacgggg gctcctatag1861 tgcggttagt ggaagaaagc acattgtggt ctgcggacac atcactctgg agagtgtttc1921 caacttcctg aaggactttc tgcacaagga ccgggatgac gtcaatgtgg agatcgtttt1981 tcttcacaac atctcccccg acctggagct tgaagctctg ttcaaacgac attttactca2041 ggtggaattt tatcagggtt ccgtcctcaa tccacatgat cttgcaagag tcaagataga2101 gtcagcagat gcatgcctga tccttgccaa caagtactgc gctgacccgg atgcggagga2161 tgcctcgaat atcatgagag taatctccat aaagaactac catccgaaga taagaatcat2221 cactcaaatg ctgcagtatc acaacaaggc ccatctgcta aacatcccga gctggaattg2281 gaaagaaggt gatgacgcaa tctgcctcgc agagttgaag ttgggcttca tagcccagag2341 ctgcctggct caaggcctct ccaccatgct tgccaacctc ttctccatga ggtcattcat2401 aaagattgag gaagacacat ggcagaaata ctacttggaa ggagtctcaa atgaaatgta2461 cacagaatat ctctccagtg ccttcgtggg tctgtccttc cctactgttt gtgagctgtg2521 ttttgtgaag ctcaagctcc taatgatagc cattgagtac aagtctgcca accgagagag2581 ccgtatatta attaatcctg gaaaccatct taagatccaa gaaggtactt taggattttt2641 catcgcaagt gatgccaaag aagttaaaag ggcatttttt tactgcaagg cctgtcatga2701 tgacatcaca gatcccaaaa gaataaaaaa atgtggctgc aaacggcttg aagatgagca2761 gccgtcaaca ctatcaccaa aaaaaaagca acggaatgga ggcatgcgga actcacccaa2821 cacctcgcct aagctgatga ggcatgaccc cttgttaatt cctggcaatg atcagattga2881 caacatggac tccaatgtga agaagtacga ctctactggg atgtttcact ggtgtgcacc2941 caaggagata gagaaagtca tcctgactcg aagtgaagct gccatgaccg tcctgagtgg3001 ccatgtcgtg gtctgcatct ttggcgacgt cagctcagcc ctgatcggcc tccggaacct3061 ggtgatgccg ctccgtgcca gcaactttca ttaccatgag ctcaagcaca ttgtgtttgt3121 gggctctatt gagtacctca agcgggaatg ggagacgctt cataacttcc ccaaagtgtc3181 catattgcct ggtacgccat taagtcgggc tgatttaagg gctgtcaaca tcaacctctg3241 tgacatgtgc gttatcctgt cagccaatca gaataatatt gatgatactt cgctgcagga3301 caaggaatgc atcttggcgt cactcaacat caaatctatg cagtttgatg acagcatcgg3361 agtcttgcag gctaattccc aagggttcac acctccagga atggatagat cctctccaga3421 taacagccca gtgcacggga tgttacgtca accatccatc acaactgggg tcaacatccc3481 catcatcact gaactagtga acgatactaa tgttcagttt ttggaccaag acgatgatga3541 tgaccctgat acagaactgt acctcacgca gccctttgcc tgtgggacag catttgccgt3601 cagtgtcctg gactcactca tgagcgcgac gtacttcaat gacaatatcc tcaccctgat3661 acggaccctg gtgaccggag gagccacgcc ggagctggag gctctgattg ctgaggaaaa3721 cgcccttaga ggtggctaca gcaccccgca gacactggcc aatagggacc gctgccgcgt3781 ggcccagtta gctctgctcg atgggccatt tgcggactta ggggatggtg gttgttatgg3841 tgatctgttc tgcaaagctc tgaaaacata taatatgctt tgttttggaa tttaccggct3901 gagagatgct cacctcagca cccccagtca gtgcgcaaag aggtatgtca tcaccaaccc3961 gccctatgag tttgagctcg tgccgacgga cctgatcttc tgcttaatgc agtttgacca4021 caatgccggc cagtcccggg ccagcctgtc ccattcctcc cactcgtcgc agtcctccag4081 caagaagagc tcctctgttc actccatccc atccacagca aaccgacaga accggcccaa4141 gtccagggag tcccgggaca aacagaagta cgtgcaggaa gagcggcttg gtaccggcga4201 ctacaaggac gacgatgaca agtaagcggc cgcggggatc cagacatgat aagatacatt4261 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt4321 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac4381 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttcggatcct4441 ctagagtcga tctgcaggca tgctagcttg gcgtaatcat ggtcatagct gtttcctgtg4501 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa4561 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct4621 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga4681 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc4741 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa4801 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt4861 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa4921 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt4981 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg5041 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc5101 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc5161 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta5221 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct5281 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc5341 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa5401 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa5461 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa5521 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt5581 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac5641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc5701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc5761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata5821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc5881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc5941 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca6001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa6061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca6121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt6181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat
