• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：遗传霉素G418
• 克隆菌株：大肠杆菌DH5α
• 质粒图谱1： png/1-1P12113513I55.png

质粒序列： 点击查看序列

LOCUS       Exported                6377 bp ds-DNA     circular SYN 21-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..6377

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        192..495

                     /label=CMV enhancer

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        496..699

                     /label=CMV promoter

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             744..1460

                     /codon_start=1

                     /product="Aequorea coerulescens GFP"

                     /label=AcGFP1

                     /note="mammalian codon-optimized"

                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK

                     FICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG

                     NYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIKV

                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYFG

                     FVTAAAITHGMDELYK"

     polyA_signal    1475..1596

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      1697..2125

                     /direction=RIGHT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2139..2468

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     CDS             2535..3329

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /label=Neo

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

     polyA_signal    3503..3624

                     /label=SV40 poly(A) signal

                     /note="SV40 polyadenylation signal"

     rep_origin      complement(4075..4660)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4831..5691)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5692..5796)

                     /gene="bla"

                     /label=AmpR promoter

     promoter        6211..74

                     /label=U6 promoter

                     /note="RNA polymerase III promoter for human U6 snRNA"

ORIGIN

        1 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg gtttatatat

       61 cttgtggaaa ggacgcggga tccgagctcg gtaccaagct taagtttaaa ccgctgatca

      121 gcctcgactg tgccttctaa atagtaatca attacggggt cattagttca tagcccatat

      181 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac

      241 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc

      301 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg

      361 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat

      421 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc

      481 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt

      541 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac

      601 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc

      661 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc

      721 cgctagcgct accggtcgcc accatgagca agggcgccga gctgttcacc ggcatcgtgc

      781 ccatcctgat cgagctgaat ggcgatgtga atggccacaa gttcagcgtg agcggcgagg

      841 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc

      901 tgcctgtgcc ctggcccacc ctggtgacca ccctgagcta cggcgtgcag tgcttctcac

      961 gctaccccga tcacatgaag cagcacgact tcttcaagag cgccatgcct gagggctaca

     1021 tccaggagcg caccatcttc ttcgaggatg acggcaacta caagtcgcgc gccgaggtga

     1081 agttcgaggg cgataccctg gtgaatcgca tcgagctgac cggcaccgat ttcaaggagg

     1141 atggcaacat cctgggcaat aagatggagt acaactacaa cgcccacaat gtgtacatca

     1201 tgaccgacaa ggccaagaat ggcatcaagg tgaacttcaa gatccgccac aacatcgagg

     1261 atggcagcgt gcagctggcc gaccactacc agcagaatac ccccatcggc gatggccctg

     1321 tgctgctgcc cgataaccac tacctgtcca cccagagcgc cctgtccaag gaccccaacg

     1381 agaagcgcga tcacatgatc tacttcggct tcgtgaccgc cgccgccatc acccacggca

     1441 tggatgagct gtacaagtga ggactagata actgaacttg tttattgcag cttataatgg

     1501 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc

     1561 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtggga agacaatagc

     1621 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc

     1681 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt

     1741 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc

     1801 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct

     1861 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat

     1921 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc

     1981 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc

     2041 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg

     2101 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa

     2161 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa

     2221 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca

     2281 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca

     2341 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg

     2401 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct

     2461 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat

     2521 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg

     2581 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg

     2641 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg

     2701 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc

     2761 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg

     2821 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca

     2881 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc

     2941 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg

     3001 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg

     3061 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata

     3121 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg

     3181 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat

     3241 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct

     3301 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca

     3361 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt

     3421 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat

     3481 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag

     3541 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt

     3601 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt

     3661 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca

     3721 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact

     3781 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct

     3841 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc

     3901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca

     3961 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg

     4021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca

     4081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa

     4141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc

     4201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc

     4261 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct

     4321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg

     4381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag

     4441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta

     4501 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg

     4561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt

     4621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc

     4681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt

     4741 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta