• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：              Homo sapiens neutrophil cytosolic factor 1 (NCF1), mRNA
• 质粒图谱1： png/1-1P2241512015N.png

质粒序列： 点击查看序列

LOCUS       Exported                4676 bp ds-DNA     circular SYN 27-JAN-2018

FEATURES             Location/Qualifiers

     source          1..4676

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     primer_bind     379..395

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     misc_feature    423..1589

                     /label=NCF1

     CDS             1686..2402

                     /codon_start=1

                     /product="enhanced GFP"

                     /label=EGFP

                     /note="mammalian codon-optimized"

                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK

                     FISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG

                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV

                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE

                     FVTAAGITLGMDELYK"

     primer_bind     complement(2455..2471)

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     rep_origin      complement(2857..3445)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(3616..4476)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(4477..4581)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc

      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat

      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt

      361 tttcccagtc acgacgttgt aaaacgacgg ccagtctcgc gatgcgtcga ctctagattg

      421 gcatggggga caccttcatc cgtcacatcg ccctgctggg ctttgagaag cgcttcgtac

      481 ccagccagca ctatgtgtac atgttcctgg tgaaatggca ggacctgtcg gagaaggtgg

      541 tctaccggcg cttcaccgag atctacgagt tccataaaac cttaaaagaa atgttcccta

      601 ttgaggcagg ggcgatcaat ccagagaaca ggatcatccc ccacctccca gctcccaagt

      661 ggtttgacgg gcagcgggcc gccgagaacc gccagggcac acttaccgag tactgcagca

      721 cgctcatgag cctgcccacc aagatctccc gctgtcccca cctcctcgac ttcttcaagg

      781 tgcgccctga tgacctcaag ctccccacgg acaaccagac aaaaaagcca gagacatact

      841 tgatgcccaa agatggcaag agtaccgcga cagacatcac cggccccatc atcctgcaga

      901 cgtaccgcgc cattgccaac tacgagaaga cctcgggctc cgagatggct ctgtccacgg

      961 gggacgtggt ggaggtcgta gagaagagcg agagcggttg gtggttctgt cagatgaaag

     1021 caaagcgagg ctggatccca gcgtccttcc tcgagcccct ggacagtcct gacgagacgg

     1081 aagaccctga gcccaactat gcaggtgagc catacgtcgc catcaaggcc tacactgctg

     1141 tggaggggga cgaggtgtcc ctgctcgagg gtgaagctgt tgaggtcatt cacaagctcc

     1201 tggacagctg gtgggtcatc aggaaagacg acgtcacagg ctacttcccg tccatgtacc

     1261 tgcaaaagtc agggcaagac gtgtcccagg cccaacgcca gatcaagcgg ggggcgccgc

     1321 cccgcaggtc gtccatccgc aacgcgcaca gcatccacca gcggtcgcgg aagcgcctca

     1381 gccaggacgc ctatcgccgc aacagcgtcc gttttctgca gcagcgacgc cgccaggcgc

     1441 ggccgggacc gcagagcccc gggagcccgc tcgaggagga gcggcagacg cagcgctcta

     1501 aaccgcagcc ggcggtgccc ccgcggccga gcgccgacct catcctgaac cgctgcagcg

     1561 agagcaccaa gcggaagctg gcgtctgccg gtggttcagg tggtggtggt tcaggtggtg

     1621 gtggttcagg tggaggagga tcaggaggag gaggatcagg aggaggagga tcaggaggag

     1681 aattcgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg

     1741 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct

     1801 acggcaagct gaccctgaag ttcatctcca ccaccggcaa gctgcccgtg ccctggccca

     1861 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga

     1921 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct

     1981 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc

     2041 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc

     2101 acaagctgga gtacaactac aactgccaca acgtctatat catggccgac aagcagaaga

     2161 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg

     2221 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc

     2281 actacctgag cacctgctcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg

     2341 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt

     2401 aagcggccgc aagcttatcg gatgccggga ccgacgagtg cagaggcggc aagggtcata

     2461 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag

     2521 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg

     2581 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca

     2641 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc

     2701 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg

     2761 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa

     2821 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga

     2881 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag

     2941 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct

     3001 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg

     3061 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc

     3121 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt

     3181 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta

     3241 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac

     3301 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc

     3361 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat

     3421 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc

     3481 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt

     3541 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta

     3601 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct

     3661 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg

     3721 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga

     3781 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt

     3841 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt

     3901 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt

     3961 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat

     4021 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc

     4081 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc

     4141 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat

     4201 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag

     4261 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt

     4321 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc

     4381 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa

     4441 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg

     4501 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa

     4561 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac

     4621 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc

//