• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：HIS3
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：              pUG34是一个酵母表达质粒。
• 质粒图谱1： png/8-1P12QF52AD.png

质粒序列： 点击查看序列

LOCUS       Exported                6297 bp ds-DNA     circular SYN 28-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..6297

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     promoter        317..503

                     /gene="S. cerevisiae HIS3"

                     /label=HIS3 promoter

     CDS             504..1163

                     /codon_start=1

                     /gene="S. cerevisiae HIS3"

                     /product="imidazoleglycerol-phosphate dehydratase, required

                     for histidine biosynthesis"

                     /label=HIS3

                     /note="yeast auxotrophic marker"

                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE

                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF

                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL

                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"

     rep_origin      complement(1423..1878)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     terminator      2086..2333

                     /gene="S. cerevisiae CYC1"

                     /label=CYC1 terminator

                     /note="transcription terminator for CYC1"

     CDS             complement(2403..3116)

                     /codon_start=1

                     /product="yeast-enhanced green fluorescent protein"

                     /label=yeGFP

                     /note="codons optimized for translation in S. cerevisiae 

                     and C. albicans"

                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK

                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG

                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV

                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE

                     FVTAAGITHGMDELYK"

     promoter        complement(3123..3472)

                     /gene="S. cerevisiae MET17"

                     /gene_synonym="MET15; MET25"

                     /label=MET17 promoter

                     /note="expression is repressed by methionine"

     rep_origin      complement(3964..4552)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4723..5583)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5584..5688)

                     /gene="bla"

                     /label=AmpR promoter

     misc_feature    5725..6228

                     /label=CEN/ARS

                     /note="S. cerevisiae CEN6 centromere fused to an 

                     autonomously replicating sequence"

ORIGIN

        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca

       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgcgtg

      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc

      181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt

      241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta

      301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat

      361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata

      421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc

      481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa

      541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact

      601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga

      661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt

      721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca

      781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag

      841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag

      901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag

      961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta

     1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca

     1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct

     1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat

     1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat

     1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt

     1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt

     1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata

     1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg

     1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc

     1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa

     1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt

     1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac

     1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta

     1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg

     1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc

     1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc

     1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg

     2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cggccgcaaa ttaaagcctt

     2101 cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg

     2161 tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac

     2221 tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct tttcggttag

     2281 agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca tgactcgagg

     2341 tcgacggtat cgataagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta

     2401 gatttgtaca attcatccat accatgggta ataccagcag cagtaacaaa ttctaacaag

     2461 accatgtggt ctctcttttc gtttggatct ttggataagg cagattgagt ggataagtaa

     2521 tggttgtctg gtaacaagac tggaccatca ccaattggag tattttgttg ataatggtca

     2581 gctaattgaa cagaaccatc ttcaatgttg tgtctaattt tgaagttaac tttgatacca

     2641 ttcttttgtt tgtcagccat gatgtaaaca ttgtgagagt tatagttgta ttccaatttg

     2701 tgacctaaaa tgttaccatc ttctttaaaa tcaatacctt ttaattcgat tctattaact

     2761 aaggtatcac cttcaaactt gacttcagct ctggtcttgt agttaccgtc atctttgaaa

     2821 aaaatagttc tttcttgaac ataaccttct ggcatggcag acttgaaaaa gtcatgttgt

     2881 ttcatatgat ctgggtatct cgcaaaacat tgaacaccat aaccgaaagt agtgactaag

     2941 gttggccatg gaactggcaa tttaccagta gtacaaataa attttaaggt caatttaccg

     3001 taagtagcat caccttcacc ttcaccggag acagaaaatt tgtgaccatt aacatcacca

     3061 tctaattcaa ccaaaattgg gacaacacca gtgaataatt cttcaccttt agacattcta

     3121 gagtatggat gggggtaata gaattgtatc tatgtatctg acgaccctgt attacacgaa

     3181 ggaaatagta gatgaaaaga caagagagca agaaaaagga aaaacttcct ttctaacaga

     3241 cgctttactt aaccttatat atatcttatt tttttcatcg agcgtgttca attggacaag

     3301 gtgccacctt ttcgacactt cggttattat gttacacagt tttcatgagg atggcgcctt

     3361 gactaactta attagtcatt tgccaaccac cacagttccc caatatcgac agcttcacgt

     3421 gccatttgcc attttgcgat catgtgacct caagagaaaa agcaaaaaat aatgagagct

     3481 aagggattcg aacccttgca tccggagctc cagcttttgt tccctttagt gagggttaat

     3541 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac

     3601 aattccacac aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt

     3661 gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc

     3721 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg

     3781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt

     3841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa

     3901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc

     3961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag

     4021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt

     4081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg

     4141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg

     4201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg

     4261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac

     4321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg

     4381 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt

     4441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg

     4501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc

     4561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt

     4621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt

     4681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag

     4741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt

     4801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc

     4861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc

     4921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg

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