• 规格：10μl质粒
• 原核抗性：氨苄青霉素Amp
• 克隆菌株：大肠杆菌DH5α
• 质粒简介：           pMAD2 derivative designed for chromosomal gene ectopic complementation in Staphylococcus aureu.pECTO a Gram negative/Gram positive shuttle vector derived from pMAD2 designed to integrate DNA sequences between SAOUHSC_00278 and SAOUHSC_00279 (NCTC8325 nomenclature HG003). It carries about 1kb of both sequences. A terminator introduced between the SAOUHSC_00278 and SAOUHSC_00279 sequences prevents polar effects associated with the expression of inserted genes.This a Gram negative/Gram positive shuttle vector with two specialized replicons and antibiotic markers. pBR322 replicon and ampicillin resistance for E. coli. pE194(ts) replicon and erythromycin resistance for S. aureus. In S. aureus, the plasmid propagates at T<30°C, it becomes a suicide vector at T>37°C.
• 质粒图谱1： png/8-1P12QA614X4.png

质粒序列： 点击查看序列

LOCUS       Exported               11390 bp ds-DNA     circular SYN 28-JAN-2018

DEFINITION  synthetic circular DNA

FEATURES             Location/Qualifiers

     source          1..11390

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     CDS             complement(95..829)

                     /codon_start=1

                     /label=ermC

                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT

                     LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY

                     NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF

                     HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID

                     DLNNISFEQFLSLFNSYKLFNK"

     CDS             1241..2452

                     /codon_start=1

                     /label=replicase

                     /translation="MSHSILRVARVKGSSNTNGIQRHNQRENKNYNNKDINHEETYKNY

                     DLINAQNIKYKDKIDETIDENYSGKRKIRSDAIRHVDGLVTSDKDFFDDLSGEEIERFF

                     KDSLEFLENEYGKENMLYATVHLDERVPHMHFGFVPLTEDGRLSAKEQLGNKKDFTQLQ

                     DRFNEYVNEKGYELERGTSKEVTEREHKAMDQYKKDTVFHKQELQEVKDELQKANKQLQ

                     SGIEHMRSTKPFDYENERTGLFSGREETGRKILTADEFERLQETISSAERIVDDYENIK

                     STDYYTENQELKKRRESLKEVVNTWKEGYHEKSKEVNKLKRENDSLNEQLNVSEKFQDS

                     TVTLYRAARANFPGFEKGFNRLKEKFFNDSKFERVGQFMDVVQDNVQKVDRKREKQRTD

                     DLEM"

     CDS             complement(3971..5989)

                     /codon_start=1

                     /label=bgaB

                     /translation="MNVLSSICYGGDYNPEQWPEEIWYEDAKLMQKAGVNLVSLGIFSW

                     SKIEPSDGVFDFEWLDKVIDILYDHGVYINLGTATATTPAWFVKKYPDSLPIDESGVIL

                     SFGSRQHYCPNHPQLITHIKRLVRAIAERYKNHPALKMWHVNNEYACHVSKCFCENCAV

                     AFRKWLKERYKTIDELNERWGTNFWGQRYNHWDEINPPRKAPTFINPSQELDYYRFMND

                     SILKLFLTEKEILREVTPDIPVSTNFMGSFKPLNYFQWAQHVDIVTWDSYPDPREGLPI

                     QHAMMNDLMRSLRKGQPFILMEQVTSHVNWRDINVPKPPGVMRLWSYATIARGADGIMF

                     FQWRQSRAGAEKFHGAMVPHFLNENNRIYREVTQLGQELKKLDCLVGSRIKAEVAIIFD

                     WENWWAVELSSKPHNKLRYIPIVEAYYRELYKRNIAVDFVRPSDDLTKYKVVIAPMLYM

                     VKEGEDENLRQFVANGGTLIVSFFSGIVDENDRVHLGGYPGPLRDILGIFVEEFVPYPE

                     TKVNKIYSNDGEYDCTTWADIIRLEGAEPLATFKGDWYAGLPAVTRNCYGKGEGIYVGT

                     YPDSNYLGRLLEQVFAKHHINPILEVAENVEVQQRETDEWKYLIIINHNDYEVTLSLPE

                     DKIYQNMIDGKCFRGGELRIQGVDVAVLREHDEAGKV"

     CDS             6365..6880

                     /codon_start=1

                     /label=zone upstream

                     /translation="MKLNYRGDNMTFEEKLSQMYNEIANEISGMIPVEWEKVYTIAYVD

                     DEGGEVVFNYTKPGSEDLNYYSDIPKDCNVSKDIFKNSWFKVYRMFDELRETFKKEDLE

                     PWTSCEFDFTRKGNLKVSFDYIDWIKLGFGPSGKENYYMYKKFGILPDMEYEMEEIRAV

                     EKYVKEQE"

     terminator      7101..7172

                     /label=rrnB T1 terminator

                     /note="transcription terminator T1 from the E. coli rrnB 

                     gene"

     CDS             8947..9138

                     /codon_start=1

                     /gene="rop"

                     /product="Rop protein, which maintains plasmids at low copy

                     number"

                     /label=rop

                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA

                     DELYRSCLARFGDDGENL"

     misc_feature    9240..9380

                     /label=bom

                     /note="basis of mobility region from pBR322"

     rep_origin      complement(9566..10154)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(10325..11185)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(11186..11290)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 gaattaattc tcatgtttga cagcttatca tcgattcaca aaaaataggc acacgaaaaa

       61 caagttaagg gatgcagttt atgcatccct taacttactt attaaataat ttatagctat

      121 tgaaaagaga taagaattgt tcaaagctaa tattgtttaa atcgtcaatt cctgcatgtt

      181 ttaaggaatt gttaaattga ttttttgtaa atattttctt gtattctttg ttaacccatt

      241 tcataacgaa ataattatac ttttgtttat ctttgtgtga tattcttgat ttttttctac

      301 ttaatctgat aagtgagcta ttcactttag gtttaggatg aaaatattct cttggaacca

      361 tacttaatat agaaatatca acttctgcca ttaaaagtaa tgccaatgag cgttttgtat

      421 ttaataatct tttagcaaac ccgtattcca cgattaaata aatctcatta gctatactat

      481 caaaaacaat tttgcgtatt atatccgtac ttatgttata aggtatatta ccatatattt

      541 tataggattg gtttttagga aatttaaact gcaatatatc cttgtttaaa acttggaaat

      601 tatcgtgatc aacaagttta ttttctgtag ttttgcataa tttatggtct atttcaatgg

      661 cagttacgaa attacacctc tttactaatt caagggtaaa atggcctttt cctgagccga

      721 tttcaaagat attatcatgt tcatttaatc ttatatttgt cattatttta tctatattat

      781 gttttgaagt aataaagttt tgactgtgtt ttatattttt ctcgttcatt ataaccctct

      841 ttaatttggt tatatgaatt ttgcttatta acgattcatt ataaccactt attttttgtt

      901 tggttgataa tgaactgtgc tgattacaaa aatactaaaa atgcccatat tttttcctcc

      961 ttataaaatt agtataatta tagcacgagc tctgataaat atgaacatga tgagtgatcg

     1021 ttaaatttat actgcaatcg gatgcgatta ttgaataaaa gatatgagag atttatctaa

     1081 tttctttttt cttgtaaaaa aagaaagttc ttaaaggttt tatagttttg gtcgtagagc

     1141 acacggttta acgacttaat tacgaagtaa ataagtctag tgtgttagac tttatgaaat

     1201 ctatatacgt ttatatatat ttattatccg gaggtgtagc atgtctcatt caattttgag

     1261 ggttgccaga gttaaaggat caagtaatac aaacgggata caaagacata atcaaagaga

     1321 gaataaaaac tataataata aagacataaa tcatgaggaa acatataaaa attatgattt

     1381 gattaacgca caaaatataa agtataaaga taaaattgat gaaacgattg atgagaatta

     1441 ttcagggaaa cgtaaaattc ggtcagatgc aattcgacat gtggacggac tggttacaag

     1501 tgataaagat ttctttgatg atttaagcgg agaagaaata gaacgatttt ttaaagatag

     1561 cttggagttt ctagaaaatg aatacggtaa ggaaaatatg ctgtatgcga ctgtccatct

     1621 ggatgaaaga gtcccacata tgcactttgg ttttgtccct ttaacagagg acgggagatt

     1681 gtctgcaaaa gaacagttag gcaacaagaa agactttact caattacaag atagatttaa

     1741 tgagtatgtg aatgagaaag gttatgaact tgaaagaggc acgtccaaag aggttacaga

     1801 acgagaacat aaagcgatgg atcagtacaa gaaagatact gtatttcata aacaggaact

     1861 gcaagaagtt aaggatgagt tacagaaggc aaataagcag ttacagagtg gaatagagca

     1921 tatgaggtct acgaaaccct ttgattatga aaatgagcgt acaggtttgt tctctggacg

     1981 tgaagagact ggtagaaaga tattaactgc tgatgaattt gaacgcctgc aagaaacaat

     2041 ctcttctgca gaacggattg ttgatgatta cgaaaatatt aagagcacag actattacac

     2101 agaaaatcaa gaattaaaaa aacgtagaga gagtttgaaa gaagtagtga atacatggaa

     2161 agaggggtat cacgaaaaaa gtaaagaggt taataaatta aagcgagaga atgatagttt

     2221 gaatgagcag ttgaatgtat cagagaaatt tcaagatagt acagtgactt tatatcgtgc

     2281 tgcgagggcg aatttccctg ggtttgagaa agggtttaat aggcttaaag agaaattctt

     2341 taatgattcc aaattcgagc gtgtgggaca gtttatggat gttgtacagg ataatgtcca

     2401 gaaggtcgat agaaagcgtg agaaacagcg tacagacgat ttagagatgt agaggtactt

     2461 ttatgccgag aaaacttttt gcgtgtgaca gtccttaaaa tatacttaga gcgtaagcga

     2521 aagtagtagc gacagctatt aactttcggt tgcaaagctc taggattttt aatggacgca

     2581 gcgcatcaca cgcaaaaagg aaattggaat aaatgcgaaa tttgagatgt taattaaaga

     2641 cctttttgag gtcttttttt cttagatttt tggggttatt taggggagaa aacatagggg

     2701 ggtactacga cctcccccct aggtgtccat tgtccattgt ccaaacaaat aaataaatat

     2761 tgggttttta atgttaaaag gttgtttttt atgttaaagt gaaaaaaaca gatgttggga

     2821 ggtacagtga tggttgtaga tagaaaagaa gagaaaaaag ttgctgttac tttaagactt

     2881 acaacagaag aaaatgagat attaaataga atcaaagaaa aatataatat tagcaaatca

     2941 gatgcaaccg gtattctaat aaaaaaatat gcaaaggagg aatacggtgc attttaaaca

     3001 aaaaaagata gacagcactg gcatgctgcc tatctatgac taaattttgt taaatgtatt

     3061 agcaccgtta ttatatcatg agcgaaaatg taataaaaga aactgaaaac aagaaaaatt

     3121 caagaggacg taattggaca tttgttttat atccagaatc agcaaaagcc gagtggttag

     3181 agtatttaaa agagttacac attcaatttg tagtgtctcc attacatgat agggatactg

     3241 atacagaaga taggatgaaa aaagagcatt atcatattct agtgatgtat gagggtaata

     3301 aatcttatga acagataaaa ataattacag aagaattgaa tgcgactatt ccgcagattg

     3361 caggaagtgt gaaaggtctt gtgagatata tgcttcacat ggacgatcct aataaattta

     3421 aatatcaaaa agaagatatg atagtttatg gcggtgtaga tgttgatgaa ttattaaaga

     3481 aaacaacaac agatagatat aaattaatta aagaaatgat tgagtttatt gatgaacaag

     3541 gaatcgtaga atttaagagt ttaatggatt atgcaatgaa gtttaaattt gatgattggt

     3601 tcccgctttt atgtgataac tcggcgtatg ttattcaaga atatataaaa tcaaatcggt

     3661 ataaatctga ccgatagatt ttgaatttaa gagtgtcaca agacactctt ttttcgcacc

     3721 aacgaaaact ggtttaagcc gactgcgcaa aagacataat cgataagctt taatgcggta

     3781 gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc

     3841 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg

     3901 tactgccggg cctcttgcgg gatcgaggcc tttagcatat tatgttgcca actgtcggaa

     3961 cgagacttct ctaaaccttc ccggcttcat catgctctct taacactgct acatcaaccc

     4021 cttgaatcct caattcacct cct