• 规格：20μl质粒
• 启动子：CMV promoter
• 复制子：ori
• 质粒分类：基因文库质粒；其它基因质粒；鸡源基因质粒
• 质粒大小：8486bp
• 原核抗性：氨苄青霉素Amp
• 筛选标记：G418
• 克隆菌株：Stbl3
• 质粒简介：          pLNCX chick src Y527F质粒是一个哺乳细胞普通过表达质粒，CMV启动子驱动鸡源的src Y527F基因的表达。
• 质粒图谱1： png/1-1FQ6161040357.png

质粒序列： 点击查看序列

LOCUS       Exported                8486 bp ds-DNA    circular SYN 16-八月-2017

KEYWORDS    pLNCX chick src Y527F

FEATURES             Location/Qualifiers

     source          1..8486

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     enhancer        169..548

                     /note="CMV enhancer"

                     /note="human cytomegalovirus immediate early enhancer"

     promoter        549..752

                     /note="CMV promoter"

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             1079..2680

                     /codon_start=1

                     /note="src Y527F"

                     /translation="MGSSKSKPKDPSQRRRSLEPPDSTHHGGFPASQTPNKTAAPDTHR

                     TPSRSFGTVATEPKLFGGFNTSDTVTSPQRAGALAGGVTTFVALYDYESRTETDLSFKK

                     GERLQIVNNTEGDWWLAHSLTTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLL

                     NPENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFSS

                     LQQLVAYYSKHADGLCHRLTNVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEV

                     WMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEY

                     MSKGSLLDFLKGEMGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLV

                     CKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTK

                     GRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKDPEERPTFEYLQAFLE

                     DYFTSTEPQFQPGENL"

     misc_feature    3573..3713

                     /note="bom"

                     /note="basis of mobility region from pBR322"

     rep_origin      complement(3899..4487)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(4658..5518)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="AmpR"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(5519..5623)

                     /gene="bla"

                     /note="AmpR promoter"

     LTR             6281..6456

                     /note="5' LTR (truncated)"

                     /note="truncated long terminal repeat from Moloney murine 

                     sarcoma virus"

     misc_feature    6519..6718

                     /note="MMLV Psi"

                     /note="packaging signal of Moloney murine leukemia virus 

                     (MMLV)"

     CDS             6919..7335

                     /note="gag (truncated)"

                     /note="truncated Moloney murine leukemia virus (MMLV) gag 

                     gene lacking the start codon"

     CDS             7379..8173

                     /codon_start=1

                     /gene="aph(3')-II (or nptII)"

                     /product="aminoglycoside phosphotransferase from Tn5"

                     /note="NeoR/KanR"

                     /note="confers resistance to neomycin, kanamycin, and G418 

                     (Geneticin(R))"

                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP

                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS

                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ

                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA

                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"

ORIGIN

        1 gtggggaggc acgatggccg ctttggtcga ggcggatccg gccattagcc atattattca

       61 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatccatatc

      121 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat

      181 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt

      241 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc

      301 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac

      361 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata

      421 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc

      481 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta

      541 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac

      601 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc

      661 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc

      721 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga

      781 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg

      841 gccccaagct tatcgataag ctgatcccct gttttgccct tagactattc aagttgcctc

      901 tgtggattag ggctggaggc agctcggatg gtctgacggc cagataaggc aggaaagaca

      961 gctattggta attgtgaaat acgcttttgt ctgtgtgctg caggagctga gctgactctg

     1021 ctggtggcct cgcgtaccac tgtggccagg cggtagctgg gacgtgcagc cgaccaccat

     1081 ggggagcagc aagagcaagc ccaaggaccc cagccagcgc cggcgcagcc tggagccacc

     1141 cgacagcacc caccacgggg gattcccagc ctcgcagacc cccaacaaga cagcagcccc

     1201 cgacacgcac cgcaccccca gccgctcctt tgggaccgtg gccaccgagc ccaagctctt

     1261 cgggggcttc aacacttctg acaccgtcac gtcgccgcag cgtgccgggg cactggctgg

     1321 cggcgtcacc actttcgtgg ctctctatga ctacgagtcc cggactgaaa cggacttgtc

     1381 cttcaagaaa ggagaacgcc tgcagattgt caacaacacg gaaggtgact ggtggctggc

     1441 tcattccctc actacaggac agacgggcta catccccagt aactatgtcg cgccctcaga

     1501 ctccatccag gctgaagagt ggtactttgg gaagatcact cgtcgggagt ccgagcggct

     1561 gctgctcaac cccgaaaacc cccggggaac cttcttggtc cgggagagcg agacgacaaa

     1621 aggtgcctat tgcctctccg tttctgactt tgacaacgcc aaggggctca atgtgaagca

     1681 ctacaagatc cgcaagctgg acagcggcgg cttctacatc acctcacgca cacagttcag

     1741 cagcctgcag cagctggtgg cctactactc caaacatgct gatggcttgt gccaccgcct

     1801 gaccaacgtc tgccccacgt ccaagcccca gacccaggga ctcgccaagg acgcgtggga

     1861 aatcccccgg gagtcgctgc ggctggaggt gaagctgggg cagggctgct ttggagaggt

     1921 ctggatgggg acctggaacg gcaccaccag agtggccata aagactctga agcccggcac

     1981 catgtccccg gaggccttcc tgcaggaagc ccaagtgatg aagaagctcc ggcatgagaa

     2041 gctggttcag ctgtacgcag tggtgtcgga agagcccatc tacatcgtca ctgagtacat

     2101 gagcaagggg agcctcctgg atttcctgaa gggagagatg ggcaagtacc tgcggctgcc

     2161 acagctcgtc gatatggctg ctcagattgc atccggcatg gcctatgtgg agaggatgaa

     2221 ctacgtgcac cgagacctgc gggcggccaa catcctggtg ggggagaacc tggtgtgcaa

     2281 ggtggctgac tttgggctgg cacgcctcat cgaggacaac gagtacacag cacggcaagg

     2341 tgccaagttc cccatcaagt ggacagcccc cgaggcagcc ctctatggcc ggttcaccat

     2401 caagtcggat gtctggtcct tcggcatcct gctgactgag ctgaccacca agggccgggt

     2461 gccataccca gggatggtca acagggaggt gctggaccag gtggagaggg gctaccgcat

     2521 gccctgcccg cccgagtgcc ccgagtcgct gcatgacctc atgtgccagt gctggcggaa

     2581 ggaccctgag gagcggccca cttttgagta cctgcaggcc ttcctggagg actacttcac

     2641 ctcgacagag ccccagttcc agcctggaga gaacctatag atctgcgcga gcaaaattta

     2701 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg

     2761 ttttgcgctg cttcgcatcg ataggcctaa cacgagccat agataaaata aaagatttta

     2821 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct

     2881 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga

     2941 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc

     3001 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag

     3061 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt

     3121 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc

     3181 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga

     3241 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct

     3301 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca

     3361 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg

     3421 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag

     3481 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag

     3541 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat

     3601 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc

     3661 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt

     3721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag

     3781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca

     3841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt

     3901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc

     3961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct

     4021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg

     4081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca

     4141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact

     4201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta

     4261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta

     4321 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct

     4381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt

     4441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga

     4501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca

     4561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat

     4621 caatctaaag tatatatgag ta