• 规格：20μl质粒
• 原核抗性：氨苄青霉素Amp
• 筛选标记：嘌呤霉素Puro
• 克隆菌株：大肠杆菌Stbl3
• 质粒简介：               HA-HIF1alpha-wt-pBabe-puro是一个人源基因哺乳表达质粒。
• 质粒图谱1： png/1-1F92QH525455.png

质粒序列： 点击查看序列

LOCUS       Exported                7703 bp ds-DNA     circular SYN 28-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    HA-HIF1alpha-wt-pBabe-puro

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7703)

  AUTHORS   .

  TITLE     Direct Submission

  JOURNAL   Exported Sep 28, 2017 from MiaoLingPlasmid

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..7703

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     LTR             8..477

                     /note="long terminal repeat from Moloney murine leukemia 

                     virus"

     misc_feature    542..741

                     /label=MMLV Psi

                     /note="packaging signal of Moloney murine leukemia virus 

                     (MMLV)"

     CDS             934..1350

                     /label=gag (truncated)

                     /note="truncated Moloney murine leukemia virus (MMLV) gag 

                     gene lacking the start codon"

     CDS             1379..1405

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /label=HA

                     /translation="YPYDVPDYA"

     misc_feature    1406..3904

                     /label=HIF1alpha

     promoter        3947..4276

                     /label=SV40 promoter

                     /note="SV40 enhancer and early promoter"

     rep_origin      4127..4262

                     /label=SV40 ori

                     /note="SV40 origin of replication"

     CDS             4286..4885

                     /codon_start=1

                     /gene="pac from Streptomyces"

                     /product="puromycin N-acetyltransferase"

                     /label=PuroR

                     /note="confers resistance to puromycin"

                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"

     LTR             4984..5577

                     /note="long terminal repeat from Moloney murine leukemia 

                     virus"

     rep_origin      complement(5949..6537)

                     /direction=LEFT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(6715..7575)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(7576..7680)

                     /gene="bla"

                     /label=AmpR promoter

ORIGIN

        1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca

       61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt

      121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag

      181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc

      241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga

      301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc

      361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc

      421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt

      481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg

      541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta

      601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa

      661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc

      721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg

      781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt

      841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt

      901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt

      961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc

     1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg

     1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca

     1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct

     1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt

     1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt

     1321 tatccagccc tcactccttc tctaggcgcc ggccatcgag actaagctta ccatggccta

     1381 cccctacgac gtgcccgact acgcctccct cggatccgcc accatggagg gcgccggcgg

     1441 cgcgaacgac aagaaaaaga taagttctga acgtcgaaaa gaaaagtctc gagatgcagc

     1501 cagatctcgg cgaagtaaag aatctgaagt tttttatgag cttgctcatc agttgccact

     1561 tccacataat gtgagttcgc atcttgataa ggcctctgtg atgaggctta ccatcagcta

     1621 tttgcgtgtg aggaaacttc tggatgctgg tgatttggat attgaagatg acatgaaagc

     1681 acagatgaat tgcttttatt tgaaagcctt ggatggtttt gttatggttc tcacagatga

     1741 tggtgacatg atttacattt ctgataatgt gaacaaatac atgggattaa ctcagtttga

     1801 actaactgga cacagtgtgt ttgattttac tcatccatgt gaccatgagg aaatgagaga

     1861 aatgcttaca cacagaaatg gccttgtgaa aaagggtaaa gaacaaaaca cacagcgaag

     1921 cttttttctc agaatgaagt gtaccctaac tagccgagga agaactatga acataaagtc

     1981 tgcaacatgg aaggtattgc actgcacagg ccacattcac gtatatgata ccaacagtaa

     2041 ccaacctcag tgtgggtata agaaaccacc tatgacctgc ttggtgctga tttgtgaacc

     2101 cattcctcac ccatcaaata ttgaaattcc tttagatagc aagactttcc tcagtcgaca

     2161 cagcctggat atgaaatttt cttattgtga tgaaagaatt accgaattga tgggatatga

     2221 gccagaagaa cttttaggcc gctcaattta tgaatattat catgctttgg actctgatca

     2281 tctgaccaaa actcatcatg atatgtttac taaaggacaa gtcaccacag gacagtacag

     2341 gatgcttgcc aaaagaggtg gatatgtctg ggttgaaact caagcaactg tcatatataa

     2401 caccaagaat tctcaaccac agtgcattgt atgtgtgaat tacgttgtga gtggtattat

     2461 tcagcacgac ttgattttct cccttcaaca aacagaatgt gtccttaaac cggttgaatc

     2521 ttcagatatg aaaatgactc agctattcac caaagttgaa tcagaagata caagtagcct

     2581 ctttgacaaa cttaagaagg aacctgatgc tttaactttg ctggccccag ccgctggaga

     2641 cacaatcata tctttagatt ttggcagcaa cgacacagaa actgatgacc agcaacttga

     2701 ggaagtacca ttatataatg atgtaatgct cccctcaccc aacgaaaaat tacagaatat

     2761 aaatttggca atgtctccat tacccaccgc tgaaacgcca aagccacttc gaagtagtgc

     2821 tgaccctgca ctcaatcaag aagttgcatt aaaattagaa ccaaatccag agtcactgga

     2881 actttctttt accatgcccc agattcagga tcagacacct agtccttccg atggaagcac

     2941 tagacaaagt tcacctgagc ctaatagtcc cagtgaatat tgtttttatg tggatagtga

     3001 tatggtcaat gaattcaagt tggaattggt agaaaaactt tttgctgaag acacagaagc

     3061 aaagaaccca ttttctactc aggacacaga tttagacttg gagatgttag ctccctatat

     3121 cccaatggat gatgacttcc agttacgttc cttcgatcag ttgtcaccat tagaaagcag

     3181 ttccgcaagc cctgaaagcg caagtcctca aagcacagtt acagtattcc agcagactca

     3241 aatacaagaa cctactgcta atgccaccac taccactgcc accactgatg aattaaaaac

     3301 agtgacaaaa gaccgtatgg aagacattaa aatattgatt gcatctccat ctcctaccca

     3361 catacataaa gaaactacta gtgccacatc atcaccatat agagatactc aaagtcggac

     3421 agcctcacca aacagagcag gaaaaggagt catagaacag acagaaaaat ctcatccaag

     3481 aagccctaac gtgttatctg tcgctttgag tcaaagaact acagttcctg aggaagaact

     3541 aaatccaaag atactagctt tgcagaatgc tcagagaaag cgaaaaatgg aacatgatgg

     3601 ttcacttttt caagcagtag gaattggaac attattacag cagccagacg atcatgcagc

     3661 tactacatca ctttcttgga aacgtgtaaa aggatgcaaa tctagtgaac agaatggaat

     3721 ggagcaaaag acaattattt taataccctc tgatttagca tgtagactgc tggggcaatc

     3781 aatggatgaa agtggattac cacagctgac cagttatgat tgtgaagtta atgctcctat

     3841 acaaggcagc agaaacctac tgcagggtga agaattactc agagctttgg atcaagttaa

     3901 ctgacaattc tgcagatatc catcacagtg gtcgaccctg tggaatgtgt gtcagttagg

     3961 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta

     4021 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat

     4081 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac

     4141 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga

     4201 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg

     4261 cctaggcttt tgcaaaaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac

     4321 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc

     4381 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact

     4441 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc

     4501 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg

     4561 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct

     4621 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc

     4681 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga

     4741 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga

     4801 gcggctcggc ttcaccgtca ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg

     4861 catgacccgc aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag

     4921 gagcgcacga ccccatgcat cgataaaata aaagatttta tttagtctcc agaaaaaggg