• 规格：20μl质粒
• 启动子：H1 promoter
• 复制子：pUC ori,F1 ori,SV40 ori
• 终止子：5LTR (truncated) ,SV40 poly(A) signal
• 质粒分类：RNA文库质粒；RNA其它质粒；shRNA其它质粒
• 质粒大小：7102bp
• 原核抗性：Amp（100μg/ml）
• 筛选标记：Puro
• 克隆菌株：Stbl3
• 表达宿主：293T等哺乳细胞
• 诱导方式：无需诱导
• 5测序引物：H1-F（TCGCTATGTGTTCTGGGAAA）
• 3测序引物：SV40-polyA-R（GAAATTTGTGATGCTATTGC）
• 备注：本质粒由淼灵质粒平台提供
• 质粒简介：         pSIH1-ControlshRNA质粒是pSIH1-H1-puro载体的阳性对照质粒，pSIH1-H1-puro是一个哺乳细胞慢病毒RNA干扰质粒。H1启动shRNA的转录，可特异性的干扰目的蛋白的表达。该质粒可作为普通过表达质粒直接转染哺乳细胞，也可包装成慢病毒侵染细胞。质粒本身带有Puro筛选标记，可用嘌呤霉素筛选转染后的细胞得到阳性克隆。
  
• 质粒图谱1： png/1-1FF91I221Y7.png

质粒序列： 点击查看序列

LOCUS       Exported                7102 bp ds-DNA    circular SYN 09-七月-2017

DEFINITION  .

ACCESSION   .

VERSION     .

KEYWORDS    pSIH1-ControlshRNA

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 7102)

  AUTHORS   MiaoLingBio

  TITLE     Direct Submission

  JOURNAL   Exported 2017年7月9日 from MiaoLingPlasmid

            http://www.snapgene.com

FEATURES             Location/Qualifiers

     source          1..7102

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     LTR             234..414

                     /note="5' LTR (truncated)"

                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"

     misc_feature    458..583

                     /note="HIV-1 Psi"

                     /note="packaging signal of human immunodeficiency virus 

                     type 1"

     misc_feature    1076..1309

                     /note="RRE"

                     /note="The Rev response element (RRE) of HIV-1 allows for 

                     Rev-dependent mRNA export from the nucleus to the 

                     cytoplasm."

     misc_feature    1798..1915

                     /note="cPPT/CTS"

                     /note="central polypurine tract and central termination 

                     sequence of HIV-1"

     promoter        2005..2208

                     /note="CMV promoter"

                     /note="human cytomegalovirus (CMV) immediate early 

                     promoter"

     CDS             2279..2878

                     /codon_start=1

                     /gene="pac from Streptomyces alboniger"

                     /product="puromycin N-acetyltransferase"

                     /note="Puro"

                     /note="confers resistance to puromycin"

                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER

                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA

                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS

                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"

     misc_feature    2885..3473

                     /note="WPRE"

                     /note="woodchuck hepatitis virus posttranscriptional 

                     regulatory element"

     misc_feature    3726..3816

                     /note="H1 promoter"

     misc_feature    3825..3881

                     /note="ControlshRNA"

     LTR             3912..4092

                     /note="5' LTR (truncated)"

                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"

     polyA_signal    4164..4285

                     /note="SV40 poly(A) signal"

                     /note="SV40 polyadenylation signal"

     rep_origin      4304..4439

                     /note="SV40 ori"

                     /note="SV40 origin of replication"

     rep_origin      complement(4879..5467)

                     /direction=LEFT

                     /note="ori"

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     CDS             complement(5638..6498)

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /note="Amp"

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     promoter        complement(6499..6603)

                     /gene="bla"

                     /note="Amp promoter"

ORIGIN

        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca

       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta

      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga

      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc

      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta

      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact

      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg

      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct

      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt

      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag

      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt

      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt

      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg

      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag

      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag

      901 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg

      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag

     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag

     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc

     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga

     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc

     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg

     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata

     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa

     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga

     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa

     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat

     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt

     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg

     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt

     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc

     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga

     1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac

     1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga

     2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg

     2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg

     2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac

     2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagaat

     2281 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg

     2341 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg

     2401 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat

     2461 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag

     2521 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc

     2581 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc

     2641 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag

     2701 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga

     2761 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga

     2821 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt

     2881 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt

     2941 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc

     3001 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga

     3061 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc

     3121 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct

     3181 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg

     3241 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct

     3301 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc

     3361 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgca

     3421 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat

     3481 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca

     3541 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgat gtcaagaatt

     3601 ggaacgctga cgtcatcaac ccgctccaag gaatcgcggg cccagtgtca ctaggcggga

     3661 acacccagcg cgcgtgcgcc ctggcaggaa gatggctgtg agggacaggg gagtggcgcc

     3721 ctgcaatatt tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg aaatgtcttt

     3781 ggatttggga atcttataag ttctgtatga gaccacttgg atccaatagc gactaaacac

     3841 atcaattcaa gagattgatg tgtttagtcg ctattttttt tgaattcttc gatctgcttt

     3901 ttgcttctac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac

     3961 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg

     4021 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga

     4081 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga

     4141 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa

     4201 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt

     4261 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc