• 商品货号：FH1156

• 规格：10μl质粒
• 质粒图谱1： png/1-1F930154949225.png

质粒序列： 点击查看序列

LOCUS       Exported                4474 bp ds-DNA     circular SYN 30-SEP-2017

DEFINITION  synthetic circular DNA

ACCESSION   .

VERSION     .

KEYWORDS    .

SOURCE      synthetic DNA construct

  ORGANISM  synthetic DNA construct

REFERENCE   1  (bases 1 to 4474)

  AUTHORS   .

  TITLE     Direct Submission

            http://www.fenghbio.cn

FEATURES             Location/Qualifiers

     source          1..4474

                     /organism="synthetic DNA construct"

                     /mol_type="other DNA"

     misc_feature    63..95

                     /label=myristolation signal

     misc_feature    96..1535

                     /label=akt-T308A/S473A

     CDS             1536..1562

                     /codon_start=1

                     /product="HA (human influenza hemagglutinin) epitope tag"

                     /label=HA

                     /translation="YPYDVPDYA"

     promoter        complement(1632..1650)

                     /label=T7 promoter

                     /note="promoter for bacteriophage T7 RNA polymerase"

     primer_bind     complement(1657..1673)

                     /label=M13 fwd

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     rep_origin      complement(1814..2269)

                     /direction=LEFT

                     /label=f1 ori

                     /note="f1 bacteriophage origin of replication; arrow 

                     indicates direction of (+) strand synthesis"

     promoter        2296..2400

                     /gene="bla"

                     /label=AmpR promoter

     CDS             2401..3261

                     /codon_start=1

                     /gene="bla"

                     /product="beta-lactamase"

                     /label=AmpR

                     /note="confers resistance to ampicillin, carbenicillin, and

                     related antibiotics"

                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI

                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS

                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW

                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA

                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS

                     LIKHW"

     rep_origin      3432..4020

                     /direction=RIGHT

                     /label=ori

                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

                     replication"

     primer_bind     4406..4422

                     /label=M13 rev

                     /note="common sequencing primer, one of multiple similar 

                     variants"

     promoter        4443..4461

                     /label=T3 promoter

                     /note="promoter for bacteriophage T3 RNA polymerase"

ORIGIN

        1 agctggccat tacggccgcg gccgcctcta gaggatctgg taccacgcgt atcgataagc

       61 ttatggggag cagcaagagc aagcccaagt ctagaatgaa cgacgtagcc attgtgaagg

      121 agggctggct gcacaaacga ggggaatata ttaaaacctg gcggccacgc tacttcctcc

      181 tcaagaacga tggcaccttt attggctaca aggaacggcc tcaggatgtg gatcagcgag

      241 agtccccact caacaacttc tcagtggcac aatgccagct gatgaagaca gagcggccaa

      301 ggcccaacac ctttatcatc cgctgcctgc agtggaccac agtcattgag cgcaccttcc

      361 atgtggaaac gcctgaggag cgggaagaat gggccaccgc cattcagact gtggccgatg

      421 gactcaagag gcaggaagaa gagacgatgg acttccgatc aggctcaccc agtgacaact

      481 caggggctga agagatggag gtgtccctgg ccaagcccaa gcaccgtgtg accatgaacg

      541 agtttgagta cctgaaacta ctgggcaagg gcacctttgg gaaagtgatt ctggtgaaag

      601 agaaggccac aggccgctac tatgccatga agatcctcaa gaaggaggtc atcgtcgcca

      661 aggatgaggt tgcccacacg cttactgaga accgtgtcct gcagaactct aggcatccct

      721 tccttacggc cctcaagtac tcattccaga cccacgaccg cctctgcttt gtcatggagt

      781 atgccaacgg gggcgagctc ttcttccacc tgtctcgaga gcgcgtgttc tccgaggacc

      841 gggcccgctt ctatggtgcg gagattgtgt ctgccctgga ctacttgcac tccgagaaga

      901 acgtggtgta ccgggacctg aagctggaga acctcatgct ggacaaggac gggcacatca

      961 agataacgga cttcgggctg tgcaaggagg ggatcaagga tggtgccact atgaaggcat

     1021 tctgcggaac gccggagtac ctggcccctg aggtgctgga ggacaacgac tacggccgtg

     1081 cagtggactg gtgggggctg ggcgtggtca tgtatgagat gatgtgtggc cgcctgccct

     1141 tctacaacca ggaccacgag aagctgttcg agctgatcct catggaggag atccgcttcc

     1201 cgcgcacact cggccctgag gccaagtccc tgctctccgg gctgctcaag aaggacccta

     1261 cacagaggct cggtgggggc tctgaggatg ccaaggagat catgcagcac cggttctttg

     1321 ccaacatcgt gtggcaggat gtgtatgaga agaagctgag cccacctttc aagccccagg

     1381 tcacctctga gactgacacc aggtatttcg atgaggagtt cacagctcag atgatcacca

     1441 tcacgccgcc tgatcaagat gacagcatgg agtgtgtgga cagtgagcgg aggccgcact

     1501 tcccccagtt cgcctactca gccagtggca cagcctaccc atacgatgtt ccagattacg

     1561 cttctagatg aattcacgcg taagcttatc gatgtcgacg ggcccggccg cctcggccgt

     1621 acccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg

     1681 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc

     1741 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct

     1801 gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt

     1861 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa

     1921 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag

     1981 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt

     2041 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac

     2101 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag

     2161 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg

     2221 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact

     2281 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg

     2341 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt

     2401 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct

     2461 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca

     2521 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc

     2581 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc

     2641 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg

     2701 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta

     2761 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc

     2821 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt

     2881 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg

     2941 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct

     3001 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc

     3061 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct

     3121 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac

     3181 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc

     3241 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat

     3301 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg

     3361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc

     3421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa

     3481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag

     3541 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta

     3601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta

     3661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag

     3721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg

     3781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg

     3841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag

     3901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc

     3961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa

     4021 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg

     4081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct

     4141 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa

     4201 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg

     4261 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag

     4321 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga

     4381 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc

     4441 gaaattaacc ctcactaaag ggaacaaaag ctgg

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